BBa_K1619001 1 BBa_K1619001 medh2 (NAD+ dependent methanol dehydrogenase) 2015-08-31T11:00:00Z 2015-09-05T11:55:31Z The gene is directly cloned from genomic DNA. Used sequence source for designing primers: http://www.genome.jp/dbget-bin/www_bget?bmet:BMMGA3_03335 Methanol dehydrogenase (medh) is an NAD-dependent dehydrogenase catalyzing the following reaction: CH3OH + NAD(+) &#8594; CH2O + NADH Or in other words, catalyzes methanol to formaldehyde with the use of NAD(+). For this enzyme to work in E. coli only this one gene is needed. The enzyme can use the E. coli's native NAD(+) resources to catalyze the reaction. The medh gene is cloned from <i>Bacillus methanolicus</i>. Specifically, the mdh2 gene. Mdh2 is chosen because of its efficiency in vivo: M??ller, J. E. N. et al. Engineering Escherichia coli for methanol conversion. Metab. Eng. 28, 190???201 (2015). The gene is successfully expressed in E. coli. false false _2036_ 27224 24669 9 false none false Marc-Sven Roell, Caroline Skar Mj??nes, and Hanna Noordzij annotation2442686 1 Start codon range2442686 1 1 3 annotation2442685 1 Medh2 range2442685 1 1 1156 annotation2442684 1 Stop codon range2442684 1 1156 1158 BBa_K1619001_sequence 1 atgacaaacactcaaagtgcattttttatgccttcagtcaatctatttggtgcaggatcagttaatgaggttggaactcgattagctgatcttggtgtgaaaaaagctttattagttacagatgctggtcttcacggtttaggtctttctgaaaaaatttccagtattattcgtgcagctggtgtggaagtatccatttttccaaaagccgaaccaaatccaaccgataaaaacgtcgcagaaggtttagaagcgtataacgctgaaaactgtgacagcattgtcactctgggcggcggaagttcacatgatgccggaaaagccattgcattagtagctgctaatggtggaaaaattcacgattatgaaggtgtcgatgtatcaaaagaaccaatggtcccgctaattgcgattaatacaacagctggtacaggcagtgaattaactaaattcacaatcatcacagatactgaacgcaaagtgaaaatggccattgtggataaacatgtaacacctacactttcaatcaacgacccagagctaatggttggaatgcctccgtccttaactgctgctactggattagatgcattaactcatgcaattgaagcatatgtttcaactggtgctactccaattacagatgcacttgcaattcaggcgatcaaaatcatttctaaatacttgccgcgtgcagttgcaaatggaaaagacattgaagcacgtgaacaaatggccttcgctcaatcattagctggcatggcattcaataacgcgggtttaggctatgttcatgcgattgcacaccaattaggaggattctacaacttccctcatggcgtttgcaatgcggtccttctgccatatgtatgtcgatttaacttaatttctaaagtggaacgttatgcagaaatcgctgcttttcttggtgaaaatgtcgacggtctaagtacgtacgatgcagctgaaaaagctattaaagcgatcgaaagaatggctaaagaccttaacattccaaaaggctttaaagaactaggtgctaaagaagaagacattgagactttagctaagaatgcgatgaaagatgcatgtgcattaacaaatcctcgtaaacctaagttagaagaagtcatccaaattattaaaaatgcgatgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z