BBa_K1621004
1
BBa_K1621004
gag/tat/pol/env - polyepitopic antigen derived from HIV-1
2015-08-27T11:00:00Z
2015-09-15T05:46:27Z
The sequence for this part was obtained from Rahimi et al. (2015) and synthesized by Integrated DNA Technologies.
There are many epitopes known from the Human Immunodeficiency Virus 1, comprising envelope proteins as well as glycoproteins amongst others. For our purposes we found a paper introducing a multi-epitopic recombinant protein that is constructed with six different epitopes. The single epitopes are two of the HIV-1-trans-activating (tat) encoding region, one epitope of the reverse transcriptase, one of the p24 protein, one of the envelope protein gp41 and one of gp120.
The HI Virus itself is highly immunogenic and underlies strict safety regulations. The great advantage of the multi-epitopic protein is that it does not have any immunogenic properties and is only built with small parts of functional proteins but still combines some of the most important epitopes present on the virus.
false
false
_2038_
24643
25600
9
false
The sequence was codon optimized for expression in E.coli with the codon optimization tool from IDT.
false
Lara Stuehn, Ramona Emig, Julika Neumann, Rabea Jesser
annotation2439314
1
HIV gag/tat/pol/env epitopes
range2439314
1
4
543
BBa_K1621004_sequence
1
atggggattagctatggccgcaagaaacgtcgccaacgtcgtcgtgcgcatcagaacatggaaccagtagacccgcgtctggagccgtggaaacatccgggctctcagccgaaaaccgcagcgtatccgcaaggatggaaaggaagcccggcgattttccagtctagcatgaccaaaatcctggagccattccggaaacaaaacccggacattgtcatttatcaatacatggatgatttgtacatggtgggtgcggcagcgaaggagccgttccgtgattacgtagatcgtttttataaaacccttcgggctgaacaagcaagccaggaagttaagaactggatgacggcggcctatcaggctcgcattttggcggtagaacgttacctgaaagaccaacagctgctgggcatttggggctgttcaggaaagctgatctgcacgactgcggtgccgtggtgtacgcgtccgaacaacaacacccgcaaacgtatccgtattcaacggggtcctggtcgcgcgttcgtaaccattggaaagatc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z