LOCUS BBa_K1621009 5012 bp DNA linear 29-Apr-2024 DEFINITION pOP - protein expression vector compatible with RFC[25] ACCESSION BBa_K1621009 VERSION BBa_K1621009.1 FEATURES Location/Qualifiers misc_feature 1..30 /label=BioBrick suffix RFC[25] misc_feature 4984..5012 /label=BioBrick prefix RFC[25] misc_feature 105..151 /label=T7 terminator primer_bind 159..178 /label=VR CDS 339..1196 /label=beta-lactamase misc_feature 1354..1973 /label=ColE1 ori CDS 3391..4470 /label=lacI promoter 4857..4873 /label=T7 promoter RBS 4876..4900 /label=RBS primer_bind 4906..4924 /label=VF2 ORIGIN 1 accggttaat actagtagcg gccgctgcag acacgagtga gatccggctg ctaacaaagc 61 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg 121 ggcctctaaa cgggtcttga ggggtttttt gctgaaaggc tcactcaaag gcggtaatga 181 ggaactatat ccggattggc gaatgggcag gtggcacttt tcggggaaat gtgcgcggaa 241 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 301 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 361 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 421 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 481 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 541 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 601 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 661 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 721 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 781 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 841 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcggcaatg gcaacaacgt 901 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 961 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 1021 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 1081 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 1141 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 1201 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 1261 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 1321 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 1381 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 1441 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 1501 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 1561 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 1621 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 1681 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 1741 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 1801 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 1861 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 1921 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 1981 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 2041 attctgtgga taaccgttaa tccgccaatg agtgtcgtga taccgctcgc cgcagccgaa 2101 cgaccgagcg cagcgagtca gtgagcgagg aagcggatga gcgcctgatg cggtattttc 2161 tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg 2221 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg 2281 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg 2341 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca 2401 ccgtcatcac cgaaacgcgc gaggctgctg cggtaaagct cattagcgtg gtcgtgaagc 2461 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt 2521 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact 2581 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag 2641 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag 2701 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc 2761 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg 2821 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca 2881 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg 2941 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag 3001 cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgtcg 3061 gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga 3121 gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag 3181 cgaaagcggt cctcgccgaa aatgacccag agcgctgtcg gcacctgtcc tacgagttgc 3241 atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag 3301 gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg 3361 agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 3421 tgcgagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 3481 cagggtggtt tttcttttca ccagtgagac gggcaaaagc tgattgccct tcaccgcctg 3541 gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg 3601 tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac 3661 taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag 3721 cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg 3781 catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg 3841 aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga 3901 acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac 3961 gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga 4021 gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg 4081 gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac 4141 cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc 4201 cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag 4261 actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg 4321 gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga 4381 aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc 4441 tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg 4501 gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac 4561 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga 4621 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca 4681 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc 4741 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg 4801 tgatgtcggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat 4861 acgactcact ataggggaat tgtgagcgga taacaattcc cctctgccac ctgacgtcta 4921 agaaagaaat aattttgttt aactttaaga aggagatata catatcgata tcggaattaa 4981 ttcgaattcg cggccgcttc tagatggccg gc //