Coupled Esterase and Terephthalic acid transporter
This part is composed of 2 composite parts of the iGEM Registry of standard parts.
This BioBrick is the assembly of : - BBa_K808007, coding for the NB-Esterase 13. This Enzyme catalyzes the cutting of PET into terephthalic acid (TPA) and ethylene glycol. - BBa_K808030, coding for the Terephthalic acid transporter. This enzyme enables the entry of TPA into our bacteria.
This BioBrick was meant to be a cluster in our Degradation.
This is no natural or even native construct. We got it synthesized by GENEART. but added the RBS via PCR.
RBS-PhoA-His6tag-pNBEst13-Myctag-EstA is a long name for a long protein construct. It is a fusion protein which is secreted into the peri plasma in order to be integrated into the outer membran. When the phoA signal sequence is cut off, the N-terminus is formed by our pNB-Est13. This esterase shows actitivty towards PET and is anchored onto the cell by its inactive EstA membrane anchor.
We got it synthesized by GENEART but added the RBS via PCR.
Marie Burghard, Henrik Cordes, Jascha Diemer, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling
putative terephtalate uptake operon2 AraProm_RBS_tctA-505_RBS_tctB-197_RBS_tphC
Valentina Herbring, Sebastian Palluk, Andreas Schmidt
araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30 Khlebnikov A, Skaug T, Keasling JD, modulation of gene expression from the arabinose-inducible araBAD promoter,J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
This promoter can be induced by arabinose. In presens of arabinose the promotor is active.
tctA_505: large subunit A2 of the tripartite tricarboxylate transporter family
tphC: terephtalate periplasmatic binding proteine of the tripartite transporter family
Wang,Y.Z., Zhou,Y. and Zylstra,G.J., Molecular analysis of isophthalate and terephthalate degradation by Comamonas testosteroni YZW-D, Environ. Health Perspect. 103 SUPPL 5, 9-12 (1995) Sasoh,M., Masai,E., Ishibashi,S., Hara,H., Kamimura,N., Miyauchi,K. and Fukuda,M., Characterization of the terephthalate degradation genes of Comamonas sp. strain E6, Appl. Environ. Microbiol. 72 (3), 1825-1832 (2006)
tphC subunit of ABC tricarboxylate transporter together with the tctA and tctB subunits it forms the complet tricarboxylate transporter.
tctB_197: small subunit B2 of the tripartite tricarboxylate transporter family
Conversion of the iGEM parts registry to SBOL2.1