BBa_K1622001
type: ComponentDefinition
persistent identity: BBa K1622001
display id: BBa_K1622001
version: 1
was generated by: iGEM to SBOL conversion
description: Coupled Esterase and Terephthalic acid transporter
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T12:31:18Z
mutable provenance: This part is composed of 2 composite parts of the iGEM Registry of standard parts.
owned by: james
owned by: myers
mutable description: This BioBrick is the assembly of : - BBa_K808007, coding for the NB-Esterase 13. This Enzyme catalyzes the cutting of PET into terephthalic acid (TPA) and ethylene glycol. - BBa_K808030, coding for the Terephthalic acid transporter. This enzyme enables the entry of TPA into our bacteria.
discontinued: false
dominant: false
experience: experience/None
group u list: _2039_
m user id: 24708
owner id: 24708
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This BioBrick was meant to be a cluster in our Degradation.
star: false
creator: Alma Chapet--Batlle, Lucas Krauss
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K808030
component: BBa_K808007
sequence annotation: BBa_K808007
sequence annotation: BBa_K808030
sequence: https://synbiohub.org/public/igem/BBa_K1622001_sequence/1

BBa_K808030
type: Component
persistent identity: component2473517
display id: component2473517
version: 1
top level: BBa_K1622001
definition: BBa_K808030
access: public

BBa_K808007
type: Component
persistent identity: component2473507
display id: component2473507
version: 1
top level: BBa_K1622001
definition: Operon2
access: public

BBa_K808007
type: SequenceAnnotation
persistent identity: annotation2473507
display id: annotation2473507
version: 1
top level: BBa_K1622001
location: https://synbiohub.org/public/igem/BBa_K1622001/annotation2473507/range2473507/1
component: BBa_K808007

https://synbiohub.org/public/igem/BBa_K1622001/annotation2473507/range2473507/1
type: Range
persistent identity: range2473507
display id: range2473507
version: 1
top level: BBa_K1622001
direction: direction/reverse
start: 1
end: 4368
orientation: inline

BBa_K808030
type: SequenceAnnotation
persistent identity: annotation2473517
display id: annotation2473517
version: 1
top level: BBa_K1622001
location: https://synbiohub.org/public/igem/BBa_K1622001/annotation2473517/range2473517/1
component: BBa_K808030

https://synbiohub.org/public/igem/BBa_K1622001/annotation2473517/range2473517/1
type: Range
persistent identity: range2473517
display id: range2473517
version: 1
top level: BBa_K1622001
direction: direction/reverse
start: 4377
end: 7419
orientation: inline

BBa_K808030
type: ComponentDefinition
persistent identity: BBa K808030
display id: BBa_K808030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: This is no natural or even native construct. We got it synthesized by GENEART. but added the RBS via PCR.
owned by: james
owned by: myers
mutable description: RBS-PhoA-His6tag-pNBEst13-Myctag-EstA is a long name for a long protein construct. It is a fusion protein which is secreted into the peri plasma in order to be integrated into the outer membran. When the phoA signal sequence is cut off, the N-terminus is formed by our pNB-Est13. This esterase shows actitivty towards PET and is anchored onto the cell by its inactive EstA membrane anchor.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We got it synthesized by GENEART but added the RBS via PCR.
star: false
creator: Marie Burghard, Henrik Cordes, Jascha Diemer, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B0029
sequence annotation: start
sequence annotation: BBa_K808028
sequence annotation: 6 histag
sequence annotation: BBa_K808026
sequence annotation: myctag
sequence annotation: BBa_K808027
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K808030_sequence/1

BBa_B0029
type: SequenceAnnotation
persistent identity: annotation2182926
display id: annotation2182926
version: 1
top level: BBa_K808030
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182926/range2182926/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K808030/annotation2182926/range2182926/1
type: Range
persistent identity: range2182926
display id: range2182926
version: 1
top level: BBa_K808030
direction: direction/forward
start: 2
end: 16
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2182940
display id: annotation2182940
version: 1
top level: BBa_K808030
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182940/range2182940/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K808030/annotation2182940/range2182940/1
type: Range
persistent identity: range2182940
display id: range2182940
version: 1
top level: BBa_K808030
direction: direction/forward
start: 23
end: 25
orientation: inline

BBa_K808028
type: SequenceAnnotation
persistent identity: annotation2182934
display id: annotation2182934
version: 1
top level: BBa_K808030
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182934/range2182934/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K808030/annotation2182934/range2182934/1
type: Range
persistent identity: range2182934
display id: range2182934
version: 1
top level: BBa_K808030
direction: direction/forward
start: 23
end: 85
orientation: inline

6 histag
type: SequenceAnnotation
persistent identity: annotation2182933
display id: annotation2182933
version: 1
top level: BBa_K808030
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182933/range2182933/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K808030/annotation2182933/range2182933/1
type: Range
persistent identity: range2182933
display id: range2182933
version: 1
top level: BBa_K808030
direction: direction/forward
start: 86
end: 103
orientation: inline

BBa_K808026
type: SequenceAnnotation
persistent identity: annotation2182935
display id: annotation2182935
version: 1
top level: BBa_K808030
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182935/range2182935/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K808030/annotation2182935/range2182935/1
type: Range
persistent identity: range2182935
display id: range2182935
version: 1
top level: BBa_K808030
direction: direction/forward
start: 104
end: 1573
orientation: inline

myctag
type: SequenceAnnotation
persistent identity: annotation2182936
display id: annotation2182936
version: 1
top level: BBa_K808030
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182936/range2182936/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K808030/annotation2182936/range2182936/1
type: Range
persistent identity: range2182936
display id: range2182936
version: 1
top level: BBa_K808030
direction: direction/forward
start: 1574
end: 1603
orientation: inline

BBa_K808027
type: SequenceAnnotation
persistent identity: annotation2182937
display id: annotation2182937
version: 1
top level: BBa_K808030
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182937/range2182937/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K808030/annotation2182937/range2182937/1
type: Range
persistent identity: range2182937
display id: range2182937
version: 1
top level: BBa_K808030
direction: direction/forward
start: 1604
end: 3037
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2182938
display id: annotation2182938
version: 1
top level: BBa_K808030
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182938/range2182938/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K808030/annotation2182938/range2182938/1
type: Range
persistent identity: range2182938
display id: range2182938
version: 1
top level: BBa_K808030
direction: direction/forward
start: 3038
end: 3040
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2182939
display id: annotation2182939
version: 1
top level: BBa_K808030
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182939/range2182939/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K808030/annotation2182939/range2182939/1
type: Range
persistent identity: range2182939
display id: range2182939
version: 1
top level: BBa_K808030
direction: direction/forward
start: 3041
end: 3043
orientation: inline

Operon2
type: ComponentDefinition
persistent identity: BBa K808007
display id: BBa_K808007
version: 1
was generated by: iGEM to SBOL conversion
description: putative terephtalate uptake operon2
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:25Z
mutable provenance: Comamonas testosteroni KF1
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: putative terephtalate uptake operon2 AraProm_RBS_tctA-505_RBS_tctB-197_RBS_tphC
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 12829
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J61101
component: BBa_K808000
component: BBa_K808004
component: BBa_J61100
component: BBa_K808005
component: BBa_K808001
sequence annotation: BBa_K808000
sequence annotation: BBa_J61100
sequence annotation: BBa_K808004
sequence annotation: BBa_K808005
sequence annotation: BBa_J61101
sequence annotation: BBa_K808001
sequence: https://synbiohub.org/public/igem/BBa_K808007_sequence/1

BBa_J61101
type: Component
persistent identity: component2201311
display id: component2201311
version: 1
top level: Operon2
definition: BBa_J61101
access: public

BBa_K808000
type: Component
persistent identity: component2201306
display id: component2201306
version: 1
top level: Operon2
definition: Ara
access: public

BBa_K808004
type: Component
persistent identity: component2201308
display id: component2201308
version: 1
top level: Operon2
definition: tctA_505aa
access: public

BBa_J61100
type: Component
persistent identity: component2201307
display id: component2201307
version: 1
top level: Operon2
definition: BBa_J61100
access: public

BBa_K808005
type: Component
persistent identity: component2201310
display id: component2201310
version: 1
top level: Operon2
definition: tctB_197aa
access: public

BBa_J61100
type: Component
persistent identity: component2201309
display id: component2201309
version: 1
top level: Operon2
definition: BBa_J61100
access: public

BBa_K808001
type: Component
persistent identity: component2201312
display id: component2201312
version: 1
top level: Operon2
definition: tphC_322aa
access: public

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2201306
display id: annotation2201306
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201306/range2201306/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K808007/annotation2201306/range2201306/1
type: Range
persistent identity: range2201306
display id: range2201306
version: 1
top level: Operon2
direction: direction/reverse
start: 1
end: 1209
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2201307
display id: annotation2201307
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201307/range2201307/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K808007/annotation2201307/range2201307/1
type: Range
persistent identity: range2201307
display id: range2201307
version: 1
top level: Operon2
direction: direction/reverse
start: 1218
end: 1229
orientation: inline

BBa_K808004
type: SequenceAnnotation
persistent identity: annotation2201308
display id: annotation2201308
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201308/range2201308/1
component: BBa_K808004

https://synbiohub.org/public/igem/BBa_K808007/annotation2201308/range2201308/1
type: Range
persistent identity: range2201308
display id: range2201308
version: 1
top level: Operon2
direction: direction/reverse
start: 1236
end: 2753
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2201309
display id: annotation2201309
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201309/range2201309/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K808007/annotation2201309/range2201309/1
type: Range
persistent identity: range2201309
display id: range2201309
version: 1
top level: Operon2
direction: direction/reverse
start: 2762
end: 2773
orientation: inline

BBa_K808005
type: SequenceAnnotation
persistent identity: annotation2201310
display id: annotation2201310
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201310/range2201310/1
component: BBa_K808005

https://synbiohub.org/public/igem/BBa_K808007/annotation2201310/range2201310/1
type: Range
persistent identity: range2201310
display id: range2201310
version: 1
top level: Operon2
direction: direction/reverse
start: 2780
end: 3373
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2201311
display id: annotation2201311
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201311/range2201311/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_K808007/annotation2201311/range2201311/1
type: Range
persistent identity: range2201311
display id: range2201311
version: 1
top level: Operon2
direction: direction/reverse
start: 3382
end: 3393
orientation: inline

BBa_K808001
type: SequenceAnnotation
persistent identity: annotation2201312
display id: annotation2201312
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201312/range2201312/1
component: BBa_K808001

https://synbiohub.org/public/igem/BBa_K808007/annotation2201312/range2201312/1
type: Range
persistent identity: range2201312
display id: range2201312
version: 1
top level: Operon2
direction: direction/reverse
start: 3400
end: 4368
orientation: inline

BBa_J61100
type: ComponentDefinition
persistent identity: BBa J61100
display id: BBa_J61100
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61100_sequence/1

Ara
type: ComponentDefinition
persistent identity: BBa K808000
display id: BBa_K808000
version: 1
was generated by: iGEM to SBOL conversion
description: araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
created: 2012-08-10T11:00:00Z
modified: 2015-05-08T01:13:25Z
mutable provenance: Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30 Khlebnikov A, Skaug T, Keasling JD, modulation of gene expression from the arabinose-inducible araBAD promoter,J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This promoter can be induced by arabinose. In presens of arabinose the promotor is active.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: non
star: false
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: AraC
sequence annotation: AraO
sequence annotation: AraO1
sequence annotation: AraPromoter
sequence: https://synbiohub.org/public/igem/BBa_K808000_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation2200757
display id: annotation2200757
version: 1
top level: Ara
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1
type: Range
persistent identity: range2200757
display id: range2200757
version: 1
top level: Ara
direction: direction/reverse
start: 1
end: 879
orientation: inline

AraO
type: SequenceAnnotation
persistent identity: annotation2200758
display id: annotation2200758
version: 1
top level: Ara
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1
type: Range
persistent identity: range2200758
display id: range2200758
version: 1
top level: Ara
direction: direction/forward
start: 909
end: 924
orientation: inline

AraO1
type: SequenceAnnotation
persistent identity: annotation2200759
display id: annotation2200759
version: 1
top level: Ara
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1
type: Range
persistent identity: range2200759
display id: range2200759
version: 1
top level: Ara
direction: direction/forward
start: 1066
end: 1077
orientation: inline

AraPromoter
type: SequenceAnnotation
persistent identity: annotation2200760
display id: annotation2200760
version: 1
top level: Ara
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1
type: Range
persistent identity: range2200760
display id: range2200760
version: 1
top level: Ara
direction: direction/forward
start: 1153
end: 1181
orientation: inline

tctA_505aa
type: ComponentDefinition
persistent identity: BBa K808004
display id: BBa_K808004
version: 1
was generated by: iGEM to SBOL conversion
description: tctA_505: large subunit A2 of the tripartite tricarboxylate transporter family
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:25Z
mutable provenance: Comamonas testosteroni KF1
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: tctA_505: large subunit A2 of the tripartite tricarboxylate transporter family
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 12829
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: deletion of PstI restriction site
star: false
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K808004_sequence/1

BBa_J61101
type: ComponentDefinition
persistent identity: BBa J61101
display id: BBa_J61101
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: fix
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61101_sequence/1

tphC_322aa
type: ComponentDefinition
persistent identity: BBa K808001
display id: BBa_K808001
version: 1
was generated by: iGEM to SBOL conversion
description: tphC: terephtalate periplasmatic binding proteine of the tripartite transporter family
created: 2012-08-10T11:00:00Z
modified: 2015-05-08T01:13:25Z
mutable provenance: Wang,Y.Z., Zhou,Y. and Zylstra,G.J., Molecular analysis of isophthalate and terephthalate degradation by Comamonas testosteroni YZW-D, Environ. Health Perspect. 103 SUPPL 5, 9-12 (1995) Sasoh,M., Masai,E., Ishibashi,S., Hara,H., Kamimura,N., Miyauchi,K. and Fukuda,M., Characterization of the terephthalate degradation genes of Comamonas sp. strain E6, Appl. Environ. Microbiol. 72 (3), 1825-1832 (2006)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: tphC subunit of ABC tricarboxylate transporter together with the tctA and tctB subunits it forms the complet tricarboxylate transporter.
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: non
star: false
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K808001
sequence: https://synbiohub.org/public/igem/BBa_K808001_sequence/1

BBa_K808001
type: SequenceAnnotation
persistent identity: annotation2179506
display id: annotation2179506
version: 1
top level: tphC_322aa
location: https://synbiohub.org/public/igem/BBa_K808001/annotation2179506/range2179506/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K808001/annotation2179506/range2179506/1
type: Range
persistent identity: range2179506
display id: range2179506
version: 1
top level: tphC_322aa
direction: direction/reverse
start: 1
end: 969
orientation: inline

tctB_197aa
type: ComponentDefinition
persistent identity: BBa K808005
display id: BBa_K808005
version: 1
was generated by: iGEM to SBOL conversion
description: tctB_197: small subunit B2 of the tripartite tricarboxylate transporter family
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:25Z
mutable provenance: Comamonas testosteroni KF1
owned by: james
owned by: myers
mutable description: tctB_197: small subunit B2 of the tripartite tricarboxylate transporter family
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 12829
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: deletion of PstI restriction site
star: false
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808005_sequence/1

https://synbiohub.org/public/igem/BBa_K1622001_sequence/1
type: Sequence
persistent identity: BBa K1622001 sequence
display id: BBa_K1622001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttttgggctaactactagagaaagaggggacatactagatggatattttcaatgctttgatggctggctttgccacggccatcacgcccgccaatctgctgtgggctctggtgggctgtgccctgggtacggccgtgggcgtgctgcccggtatcggcccggccgtggccgtggccatgctgctgcccatcaccgccaaggtggatgtgacggcatccatgatctttttcgccggcatctactacggtgccatgtatggcggctcgaccacctcgattctgctgaacacacctggcgagacggcatccatggtcactgccatggaaggcaacaagatggccaagagcggccgagccggggcggcgctggcaactgccgcgatcggctcgtttttcgcgggtacctttgccaccgtggtggtgaccctgttcgcaccctatgtggccgacttcgccgtgaaattgggcccgcccgagtacttcatgctcatggtgctggccttcaccaccgtgagtgccgtgctgggtcagagcacgctgcgcggcatgacggcgctgtttgtcggtctggctgcgggttgcatcggcatggaccagatctccggtgcggcgcgctataccggcggcaagatggagttgctggatggcatcgatatcgtgctggtggccgtgggtctgtttgccgtggccgaagtgctgtatgccgccatgtatgaaggcaaggtcgaggagtcgcagaacaagctgacgcgtgtgcacatgaccaagcgcgactggaagcgttcctggcccgcctggatgcgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K808030_sequence/1
type: Sequence
persistent identity: BBa K808030 sequence
display id: BBa_K808030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttcacacaggaaacctactagatgaaacaaagcactattgcactggcactcttaccgttactgtttacccctgtgacaaaagcgcatcaccaccatcatcacatgtatgatacaactgtcgaaacacgcttcggaaagctgaaaggcagagcggaaaacggagtccgtatctttaaaggcgttccatacgcaaaacctcccgtcggcgacctaaggtttcgggaaccgcagcgaatggaggcctgggaaggtgagctggatgcttttcaatttggcccggtttgtccgcagcctgatggggtattgcctgagtcagcgggggttcaaaagtctgaggattgcctttatttaaatgtgtacgcacccgaagaggcggacggggatctgcctgttatggtgtggattcatgggggcgctttttatcgcggcgccggaagtgaaccgctctatgacgggactcagcttgcaaagcagggaaaggtgatcgtggtcaccatcaattatcgcctcggtccgttcggttttttgcatctatcctcaattgatgattcctacagcagcaatcttggcctgctggatcaaatcgcggctttagagtgggtgaaagacaatatcgctttctttggcggtgaccgtcatcacattacggtttttggagagtcggcgggatcgatgagctgcgcttcgcttttggcaatgccgaaagcaaaggggctttttcaacaggccattatggaaagcggggcttccgcaactatgtccgataagttggcgaaagccgcagcagaaagattcttaaggattctcgatattgatcatcatcatctggagcgccttcatgatgtatctgatcaagaacttcttgaagccgccgatcagctgcgcactttaatgggagaaaatatttttgaattgatttttctgcctgcgcttgacgaaaaaaccttgccgctgaagccggaggtcgccgtcgcaaaaggcgcggcaaaagagatcaatctattaatcggaacaaaccgtgatgaaggcgtcttgttttttccctctgattcggatcttttgcctgagagcaagatcaacgagattttagaagaatacatgggtaaagaggccgccgaagccgcctcctctctgtatccgaggtcattggaaggccatgttgatatgatgacagatctgatcttttggcatccgtctgttgtgttcgcttcggctcaatcacgatatgcatctgtctttatgtaccggtttgactggcatgcggattcagagcagccgccgttcaacaaagctgcgcacggcttagagattccgtttgtatttggaaatatggacattttggaacagctgacaggtacgaaggccggtgaagaagcgcagctgcttgctgaacagatccaggctgcctgggtgtcttttgcccgatccggaaatccgagcaccgatgatgtcagctggcctgattatgatgaagattcacggaaaacgctgatttttgatcaagaggtcgcagttgaaagcgatccttattcagataagagaaagatgttgacagccccgaacccgcagattgaacaaaaactaataagcgaagaagacctagctccttcgccctattcgacgctggtggtgttcggcgacgtccaggcccaacaagccgccggtcgcctggtggatagcgtgcaggccctacaacaggccggcgcgcgctacatcgtggtctggctgttgcccgacctgggcctgaccccggctaccttcggtggtcccttgcagcctttcgccagccaactcagcggcacgttcaacgccgagctgaccgcccagttgagccaggccggcgccaacgtcattccgttgaacatcccgctgctgctcaaggaaggcatggccaacccggcttccttcggcctggccgccgaccagaacctgatcggcacctgtttcagcggcaacggctgcaccatgaacccgacctacgggatcaacggcagcacgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K808007_sequence/1
type: Sequence
persistent identity: BBa K808007 sequence
display id: BBa_K808007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttttgggctaactactagagaaagaggggacatactagatggatattttcaatgctttgatggctggctttgccacggccatcacgcccgccaatctgctgtgggctctggtgggctgtgccctgggtacggccgtgggcgtgctgcccggtatcggcccggccgtggccgtggccatgctgctgcccatcaccgccaaggtggatgtgacggcatccatgatctttttcgccggcatctactacggtgccatgtatggcggctcgaccacctcgattctgctgaacacacctggcgagacggcatccatggtcactgccatggaaggcaacaagatggccaagagcggccgagccggggcggcgctggcaactgccgcgatcggctcgtttttcgcgggtacctttgccaccgtggtggtgaccctgttcgcaccctatgtggccgacttcgccgtgaaattgggcccgcccgagtacttcatgctcatggtgctggccttcaccaccgtgagtgccgtgctgggtcagagcacgctgcgcggcatgacggcgctgtttgtcggtctggctgcgggttgcatcggcatggaccagatctccggtgcggcgcgctataccggcggcaagatggagttgctggatggcatcgatatcgtgctggtggccgtgggtctgtttgccgtggccgaagtgctgtatgccgccatgtatgaaggcaaggtcgaggagtcgcagaacaagctgacgcgtgtgcacatgaccaagcgcgactggaagcgttcctggcccgcctggatgcgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61100_sequence/1
type: Sequence
persistent identity: BBa J61100 sequence
display id: BBa_J61100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K808000_sequence/1
type: Sequence
persistent identity: BBa K808000 sequence
display id: BBa_K808000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttttgggctaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K808004_sequence/1
type: Sequence
persistent identity: BBa K808004 sequence
display id: BBa_K808004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggatattttcaatgctttgatggctggctttgccacggccatcacgcccgccaatctgctgtgggctctggtgggctgtgccctgggtacggccgtgggcgtgctgcccggtatcggcccggccgtggccgtggccatgctgctgcccatcaccgccaaggtggatgtgacggcatccatgatctttttcgccggcatctactacggtgccatgtatggcggctcgaccacctcgattctgctgaacacacctggcgagacggcatccatggtcactgccatggaaggcaacaagatggccaagagcggccgagccggggcggcgctggcaactgccgcgatcggctcgtttttcgcgggtacctttgccaccgtggtggtgaccctgttcgcaccctatgtggccgacttcgccgtgaaattgggcccgcccgagtacttcatgctcatggtgctggccttcaccaccgtgagtgccgtgctgggtcagagcacgctgcgcggcatgacggcgctgtttgtcggtctggctgcgggttgcatcggcatggaccagatctccggtgcggcgcgctataccggcggcaagatggagttgctggatggcatcgatatcgtgctggtggccgtgggtctgtttgccgtggccgaagtgctgtatgccgccatgtatgaaggcaaggtcgaggagtcgcagaacaagctgacgcgtgtgcacatgaccaagcgcgactggaagcgttcctggcccgcctggatgcgcggcacgctgctggggacaccgttcggttgcattcctgccggcggtaccgagatcccgacctttctgagctacgcgaccgaaaagaagctggccaagggcgaagacaaggccgagttcggcggcaagggcgccatcgaaggcgtggccggtccggaagccgccaacaatgcgaccgtgaccgcggctctgattccgctgctgactctgggcattcccaccagcaacaccacggccgtgctgctgggcgctttccagaactacggcatcaaccccggcccccagctgtttaccagctctgcggccctggtctgggcgctgatcgcctcgctgtacatcggcaacgtgatgctgctggtgctcaacctgcccatggtgggcctgtgggtcaagctgctgaagattccgcgtccccagctgtatgccggcattctgatctttgcaaccgtcggtgcctacggcatgcgccagagcgcattcgatctgttcctgctctacggcatcggcgtgctgggcgtgatcatgcgccgcttcgacttccccaccgctcccgtggtggtgggcatgatcctcggcccactggccgaagcccagatgcgcaacgccgtgtctatcggcgagggcagctggtggatcttccttcagcgccccatgtcgctgacgctgatcatcatcgtgctggctgtgctgatcattccccgcgtgctcaagcgcctgtcgcaggccaagcatgcccgggaggcaagcaccgcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61101_sequence/1
type: Sequence
persistent identity: BBa J61101 sequence
display id: BBa_J61101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagacaggacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K808001_sequence/1
type: Sequence
persistent identity: BBa K808001 sequence
display id: BBa_K808001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgcaacgcatccattcgcagacgcgaagcattgatcggcattgccgctgcggtcgctgcaacaggtagcctggctcagtcaaatcaacctctcaagattgtcgtgcctttctcagccggaggtaccgccgatgtactgccgcgcctagtagccgaaaaaatccgcgcggactacgcaggtggcgtgatcattgaaaacaagcccggggccggcggaaacatcggtgccgatctagtgttccgggcacaaccggacggaatgacagtgctggcctcccctccggggcccatcgccatcaaccacaacctctatcaaaagcttagcttcgaccctactcgctgggtgccagtaacgattttggccacagtgcccaacgtcttggccatcaaccccaagttaccgactaagtcacttggcgagttcattgcatacgccaaagccaaccccaaaaaagtgactgtggcgacgcaaggcgacggctctacatcccacctgacggcagccatgttcatgcagctgactggtgttgagttaaccgtcattccctacaaggggacagccccggcactgatcgacctgatcggcggcaatgtggatgtgtttttcgacaacatcagctcatcggcaacttaccaccaagccggcaaggtgcgcattctggccattgccgacgagcagcgctctcaaatattgccccaggtccccaccttcgcggagcagcaatggccagccatgcaagcagtgacgttcttttctgtcgtcgcgcccccaggcacgagctcagaaatcgctcagaagcttcagaagcagatggccctggctctgtcatcgaacgacatccgcaagcactttcaggaacagggcgccgtgccctgcggctgggatcccaccaagactgctcagttcatccgtcaggaaaccgagaagtggaagaaggtgctgaaggccgccaacgtcaagctctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K808005_sequence/1
type: Sequence
persistent identity: BBa K808005 sequence
display id: BBa_K808005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctgattccttccaagatctggcccgggcagcggaggccgcacaggtttcggcctatgcagaccccgacgagccgcagagccccaacgtaccgtccaagagagcgcaaaccattgtcggtgcgggcgtgctgctggtcgctgcgggactggcccttggcgcactgcaaattccttcggactccggctacggcggcgtcggccccaatttcctgccctgggtctgcgcagtggtgctgggtatctgcggtgccctgctgatctgggaagcacgcagcggtggttttcgccatgcggccgacccgggcggccagcccaaggtcgacatcctgccctttgcctgggtctcggccggtctgctggtgaatgcggccttgatcggtaccctgggctttattttcagctgcaccatctgctatgtgctggccgtgcagggcctgcgccgagccgcgcatcagtcgcaggcgtccgcaccgggcacctgggtcaaggatgtgatcacggggctgctgattgcggcaccggtgttctggatgtttaccaagttcctggccatcaatctgcctggtctgactgaaacggggtggctgtaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z