##gff-version 3
##sequence-region BBa_K1624001 1 3585
BBa_K1624001	.	start_codon	2512	2514	.	+	0	ID=annotation2448885;Name=start codon
BBa_K1624001	.	start_codon	107	109	.	+	0	ID=annotation2448875;Name=Start Codon
BBa_K1624001	.	engineered_region	776	2511	.	+	0	ID=annotation2448884;Name=BBa_J45017
BBa_K1624001	.	CDS	2512	3585	.	+	0	ID=annotation2448886;Name=BMST1
BBa_K1624001	.	start_codon	92	93	.	+	0	ID=annotation2448871;Name=Transcription Start
BBa_K1624001	.	sequence_feature	79	84	.	+	0	ID=annotation2448870;Name=-10 Region
BBa_K1624001	.	stop_codon	3583	3585	.	+	0	ID=annotation2448887;Name=stop codon
BBa_K1624001	.	conserved_region	23	91	.	+	0	ID=annotation2448866;Name=Protected by CooA+RNAP
BBa_K1624001	.	point_mutation	1215	1215	.	+	0	ID=annotation2448882;Name=g -> a to eliminate PstI site
BBa_K1624001	.	engineered_region	2512	3585	.	+	0	ID=annotation2448888;Name=BBa_J45004
BBa_K1624001	.	CDS	776	1081	.	+	0	ID=annotation2448878;Name=PchB
BBa_K1624001	.	conserved_region	36	61	.	+	0	ID=annotation2448867;Name=Protected by CooA
BBa_K1624001	.	non_covalent_binding_site	41	44	.	+	0	ID=annotation2448868;Name=CooA binding site
BBa_K1624001	.	start_codon	776	778	.	+	0	ID=annotation2448877;Name=pchB start codon
BBa_K1624001	.	conserved_region	99	102	.	+	0	ID=annotation2448873;Name=conserved
BBa_K1624001	.	engineered_region	95	106	.	+	0	ID=annotation2448874;Name=BBa_B0034
BBa_K1624001	.	stop_codon	2506	2511	.	+	0	ID=annotation2448883;Name=pchA double TAA stop codon
BBa_K1624001	.	CDS	1078	2511	.	+	0	ID=annotation2448880;Name=PchA
BBa_K1624001	.	engineered_region	1	94	.	+	0	ID=annotation2448872;Name=BBa_K352002
BBa_K1624001	.	non_covalent_binding_site	52	56	.	+	0	ID=annotation2448869;Name=CooA binding site
BBa_K1624001	.	sequence_feature	8	33	.	+	0	ID=annotation2448865;Name=A+T rich sequence
BBa_K1624001	.	engineered_region	107	775	.	+	0	ID=annotation2448876;Name=BBa_K352001
BBa_K1624001	.	stop_codon	1079	1081	.	+	0	ID=annotation2448881;Name=pchB stop codon
BBa_K1624001	.	start_codon	1078	1080	.	+	0	ID=annotation2448879;Name=pchA start codon
>BBa_K1624001
taatacgactcactatagggagaaaagaggagaaatactagaatgccaccacgcttcaatattgcaaacgtactgctgt
ctcctgatggtgagacgttcttccgcggttttcgctctaagattcatgcgaaaggctctctggtatgtactggtgaagg
tgatgaaaacggtgtttttgttgtggttgatggtcgcctgcgtgtttacctggttggtgaggagcgtgagattagcctg
ttctacctgacttccggcgatatgttctgcatgcattccggctgcctggttgaagccaccgagcgcaccgaagtgcgtt
tcgccgatatccgcacgttcgagcagaaactgcaaacctgtccgtctatggcatggggcctgatcgccattctgggccg
tgctctgacctcctgtatgcgtaccatcgaagacctgatgttccacgatattaaacaacgtatcgcgggctttttcatc
gaccacgctaacactaccggtcgccagactcagggtggcgtaattgtttctgttgacttcactgtagaggaaatcgcta
atctgatcggtagctcccgccagactactagcacggcgctgaactctctgattaaagagggttacatctcccgccaggg
ccgtggtcactatactatcccgaacctggttcgcctgaaggcggctgcggatggtgaccgcgatgacgatgacgattga
tcattaatttattttggtcaaggagatgatgacatttataaactcagtattctctttggacatgcaatcagaaacaatc
tcttcgtacttgtggaacactaaatccatcaattccttgtcaaagtggctgacaagcaaaggctcagccacagctctca
tgcaccttgacacattgtaaccaccattcttctcattattagaagcattccaatgaactcttgatgtttccaagcgatt
aatggtgaatgatccttccttctcaactatgtactttacttctgctggtgatggtgtgtattgaggaatattgaatgca
tctactttctcttcttttatcaatccctcttcaaccaacttattgagggccatggctagaagctcccaaatgtaacagc
attctttgctagtaggatcctcactttctctacctaggagtgttaacaccatctttccacctttcatcaattcctctga
acggtacttgagaaaatttgaaaaatctatttcatattgcttgtagtatgctttaataacacttagagggcttgttctt
gccatgtaaatgtttcccttgttattttcaatcccattaggcacctgagatagccacatgagactgtaggaggagtaaa
caaaatgtaaacttttggaagggaaaagtctagtataaaatgaaccaggcactccactgaaaaaacatggaccaaagct
ttcccctatatgctttctcaaatcttcttgaaatgcccccagtgactgaaaaagtgtattaaaatcattgccaggaaga
tcattgaagtgaaaataaaactctggagacttaaaaccatgcttttttcgttctttttctactattttaacaagctgtg
agaccaccaagaaagtgttagctcccaaagaacaacccaaatctgcaatgcataaggtttctggaaagaggctgctgta
gagatcaatcatggcttgctcagttattggctttgtcatgagaatcaccttttgctgaaccaaagaattgtttgcatag
ctactgtctccatttcctccattcatgtgaagaacttcaacaacttccattctagtatttctcctctttttattaggcg
acgccgcgctgcaagggcacttcgtccaggccgcctatggcggatagagcttcccgcatggcactgagcttaaggcagg
tttcgcgatactcgtgggccggttccgaatcgcctaccagaccgcagccggcgaacaggtagccccggcccggcgtgag
cagggccgagcgcagcgccaccaggaaatcgccgttgccttcgccgtcgagccagcccagcggcgcggcgtaccagccg
cggtccatcccttcgtgctggcggatgtagtccagcgccgcgctgcgtgggtagccgcccaccgcgggggtcggatgca
gcgcttgtagcagccgcaggatgccgcctgcgtcagcgaggcgggcgcggatcggcgtgttcaggtgctggactcgcgc
cagtcgtttcaggccgggcgcatcggggatttccagcacctcgctgaagggttccagggccgtacggatcgcctccacc
accaactggtgttcgtgcctgtccttggcgctgtccagcagggcctgtccgagccgcgcatcttcctgggcatcgccgc
cgcgggcgatggtcccggccagggcatgggtgagtgcctcgccggcgcgaatgcggaccaggcgttccggggaggcgcc
gaggaagcaggcgttgccgcggcgacaggcgaacagctgggcgtcggcatgttgcagacgcaggtgttcgatgacctgc
cacggctcgatgtcgccgagaggccgggcctgggtgcgggccagcacgaccttgccgaagcgtccctggcggacactgc
ttaccgcgtcgctcaccttggcttcccattgcctgcgctcctgcgccgaagcgtcgccctgcgcgccagcggtcggccc
cgagggccggcgtctggccggctgcctcaggcgcagtagcgccgagcagtggtaggcggccagggccagggcatcttcg
ccgggcttggccaggtgttggcagagtacccggtagcgttcgccctcgcgcagcacggtgatgccggcgagcatcaggc
tggcatcggcgaaggcttgccagtgttcctcgcgcgggccgcgcggatcgaagcggaatccgccgcacaggcgcggcgc
cagcgggccctcgaccacggcgtcggcgcagagcaattgccagttttcctcgatccgggcgaagcgctgttcgccgtgg
ccttgcagctccagggcgcagccccaggcgaagaacgccagctcgggctgttcccagtacaggcagggcgtttgccggt
cgtcccaggcaccgaatacctgtagcgggtccaatgggtcgatctcgaacgatgccgccaccagcaccggacgatcgag
cgcctgcgcctggccgatggcgcgctcgaaggtgccgcgcaaggcgtgcaggcactggctcaggggcgccagccggctc
atgcggcaccccgtgtctggcgccagtacttgatctgctcggcgatgtaccagtggatgatctgcgcgaacagtccctc
gacgaagggcgcgtcgagtccgttttcctcggcccagcgggcgcgctcggggagcatcgcggcgacccgctcgggcgcc
ggaatcgccgcctcgctggccttgaagcgcgacgccgccttgacgtagtccatgcggcggccgagggcctggacgatat
ccaggtcgatccggtcgatggcctcgcggatgtccgccaggccggtgcagtcttcgggagttttcattctagtatttct
cctctttcctggcgccgagtaagcgacaaaaagcccggccccgtctgtcggccagatgacagttatccagagtttttat
gggggtatgaaaagacgccgaa