BBa_K1627001 1 BBa_K1627001 Device to demethylate 3-methylxanthine 2015-09-09T11:00:00Z 2015-09-11T02:43:05Z The ndmB and ndmD genes were isolated from Pseudomonas putida CBB5. gst9 was isolated from Janthinobacterium sp. Marseille. This part contains genes that allow for the demethylation of 3-methylxanthine. The part contains a synthetic operon consisting of ndmB, ndmD from Pseudomonas putida CBB5, and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110. This part can be used in a ∆guaB strain of E. coli to measure the amount of 3-methylxanthine in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of 3-methylxanthine, a growth curve can be made that correlates culture growth (OD600) with concentration of 3-methylxanthine. Then if the strain is grown in a minimal media with an unknown concentration of 3-methylxanthine, the growth can be compared to the growth curve to calculate the initial concentration of 3-methylxanthine in the media. false false _2044_ 22564 22564 9 false These genes were amplified from the pDCAF3 plasmid (BBa_K734000). An EcoRI restriction site was removed from the ndmB gene during the cloning process. The genes were cloned into the pSB1C3 backbone using BioBrick Standard Assembly. false Alejandro Gutierrez annotation2449450 1 B0034 range2449450 1 2971 2982 annotation2449437 1 J23110 range2449437 1 1 35 annotation2449449 1 ndmD range2449449 1 1160 2926 annotation2449451 1 gst9 range2449451 1 2989 3663 annotation2449448 1 B0032 range2449448 1 1141 1153 annotation2449438 1 K082001 range2449438 1 44 59 annotation2449447 1 ndmB range2449447 1 65 1132 BBa_K1627001_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtatactagatgaaagaacagctcaagccgctgctagaagacaagacttaccttcgccacttctggcatcccgtgtgtacccttaatgagttcgaacgcgccaacgccagtgggcacggccccatgggcgtcaccttgctaggcgagaaattggtgttggccaggttaaattcaaagatcattgcggctgctgaccgatgtgctcatcgatcggcacagctctccatcggccgcgtttgcagcaacgccggaaaggactatctcgaatgcccgtatcacggctggcgctacgatgaggctggggcctgtcaactgatccctgcttgccctgacaaaagcatctctcctcgggccaagatttcctcattcgattgtgaggtgaaatacgacatcgtgtgggtacggctggataacagcttcgattgcactcagattccatacctcagcgatttcgataatcccgacatgcaggtaatcgttgccgattcgtatatttgggagactgttgccgagcggcggtgggagaactttacagatttttcgcactttgccttcgtacacccagggacgctctatgatccgtttttcgctagccacccaactgtttacgtgaatcgcgttgatggtgagttgcaattcaaactcgctccgccgcgtgaaatgaaaggcatcccgccagaagcaccgatgggtgacttcacctaccgctgcacaatgccgtattcagtaaatcttgaaatcaaattgtggaaagatgactcacgtttcgttctttggactaccgctagccctgtcgataacaagtcttgccggaattttatgattattgtgcgtgagaaggataatcaacctgatcatatgcacctggctttccagaagcgggtgcttgacgaagaccagcctgttatcgaatcgcaatggcctctcgaaatacagacctcggaagtctccgttgcaaccgataaaatttccgtccagttccgcaaatggcataaagagctatctctgtcagccgttgaaggacgggaggcgttccgcgattccgtcttgacgaatgttattgaagaagaacagtaatactagagtcacacaggaaagtactagatgaacaaacttgacgtcaaccagtggtttcctattgctaccactgaagatctcccgaagcgccatgtctttcatgccacgttgttggggcaagaaatggccatctggcgcgatgactctggttcagttaatgcttgggagaaccgctgcccgcatagaggattgcggttgacactgggtgctaataccggtaacgagttgcggtgtcagtatcatggatggacttatgaaagcgggactggtggctgcacttttgtcccagcccatcgcgatgcaccacccccaaatgccgcgcgggttaatacttttcctgtccgcgaaaagcacggctttatctggacgacattaggtcagccgccaggagagcccatttcaatcctcgatgacgctcagcttgtaaacgctgtaaaaacaaatctgcatagcgtagttatagatgctgatattgacggagttgtcagcgtcctacgtcagaatctttcagcgttcatcgatgtgtttggtgcggccagcgctgaagatctgcatttgaaatccatgctgcaagatcgagggattctggtaacaagatcaggctctattgctattcatttttatatgcagcgctcaaccattagtaaatgcgttgtacatgcgcaagtacttactccgggacgtccaggatacgaacttcaaaagaactactcgtatgccatgaacgttatccgcagggcagcagaagctgtagctaccgacttgattagcattacagatatcagcgatcagactatcgaaaagcttgaagtcgttagagaaaacatgactaaggctcctccaacccactatatctgcgaagtggttacgcgtactcaagagacaggtgatattaactcatactggctgaagcctatcggctacccactaccagcattcagtccagggatgcacatcagcatcacaacgccggagggtagcattcgacaatattccctcgtgaacgggcctgacgagcgtgaatccttcatcatcggtgtgaagaaagagattcagtcccgtggcggctccagatcaatgcacgaagatgtgaaggttggaacgcaactaaaagttacacttccgaggaacggttttccactcgtccaaaccagaaaacacccgattctcgtagcaggtggcatcggtatcaccccaattttgtgtatggcacaggctctggatcagcaaggttcatcgtatgaaatacattattttgctcgtgcatttgagcatgttccattccaggatcgactgactgcgttgggcgatcgtttgaatgtgcatcttggcctcggcccagacgagactagagcaaaacttgccgacatcatggagattcataacgcccaagacgtagatgtttacacttgcggcccgcaaccaatgatcgaaactgtatctgctgtcgctcttgctcatggcatcgctgaagagtccatccgatttgaatttttcagtaaaaagaacgatgttcccgtttctgatgaagaatatgaggttgagctcaaaaaaactggtcaaatattcactgtctcgcctggctctacgttgttgcaagcttgtttggacaacgatgttcgtatcgaagcttcttgtgagcagggtgtatgcgggacttgtataactccagtcgtatccggcgatctcgagcatcatgacacttacctttctaagaaagaaagggaaagcggtaagtggatcatgccgtgtgtttcgcgctgcaagtccaaaaaaatcgttctcgatctgtgaggccgggcatcacccacggtatggacgagctgtataaactagagaaagaggagaaatactagatgatcacgctttatgactatgaactctcggggaattgctacaaggtgcggatgctcctttccatcctgaagctaccgtacacgatagagacagtggaatttttcccgtccagggaacataaatcaccggccttcctgcgcatcaatccactcggccagttgccggtattgcgcgatgatgatttcgtgttgcgcgatgcccaggccatcctggtttacctggcgaacaaatacgacaccagtttgacctggtatccgacacatgcgcccgccatcctcggccaggcaaatatgtggctcgcgtttgccgatggtttgaccggctcgatttccgctgcacgtttgcatgatttgtttttttacgatttcaatgcagagcaatgccgtgcccgcgcgcacgaactactgcgcatactggacgaacacctgtgggcggcggaacaaaatggacatcaatggctatgtccggccgccgcacccaccatcgcggatcttgcctgcttcccgtatatcgcattgtcggatgaagccggcgtttcactactcgactacccggcggtacgacgctggctggaccgtgtcaaacgcatccccggattcaccgtcatgtcggggatttttccaacctcagcttccttcgatccggcctcacttgcggaagaaacctgtcaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z