BBa_K1627002 1 BBa_K1627002 Device to demethylate 7-methylxanthine 2015-09-09T11:00:00Z 2015-09-11T02:56:04Z The ndmC and ndmD genes were isolated from Pseudomonas putida CBB5. gst9 was isolated from Janthinobacterium sp. Marseille. This part contains genes that allow for the demethylation of 7-methylxanthine. The part contains a synthetic operon consisting of ndmC, ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110. This part can be used in a ∆guaB strain of E. coli to measure the amount of 7-methylxanthine in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of 7-methylxanthine, a growth curve can be made that correlates culture growth with concentration of 7-methylxanthine. Then if the strain is grown in a minimal media with an unknown concentration of 7-methylxanthine, the growth can be compared to the growth curve to calculate the initial concentration of 7-methylxanthine in the media. false false _2044_ 22564 22564 9 false These genes were amplified from the pDCAF3 plasmid (BBa_K734000). The genes were cloned into the pSB1C3 backbone using BioBrick Standard Assembly. false Alejandro Gutierrez annotation2449452 1 J23110 range2449452 1 1 35 annotation2449492 1 B0034 range2449492 1 2758 2769 annotation2449493 1 gst9 range2449493 1 2776 3450 annotation2449490 1 B0032 range2449490 1 928 940 annotation2449491 1 ndmD range2449491 1 947 2713 annotation2449489 1 ndmC range2449489 1 65 919 annotation2449488 1 B0030 range2449488 1 44 58 BBa_K1627002_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagattaaagaggagaaatactagatgtctactgaccaagtaatttttaacgactggcatccagtcgctgctttggaagatgtatctctcgataaacgataccgctgtcgactactaggtcgtacagttagttatgtgaaaacatctgatgctgttaatgctcattgggaagaaagtgcagatgaaattaagaccatccgcgctaaagaaatctatggtcttctgtggctctcctttgccgacaaacccagtgagatgttcgatattgcagagttcaaggaacctgatcgccgaatcgtcagcgctggatctgtgcgcgtaaatgtctcaggactgcgtgctatcgaaaactttctggacatggctcatttcccttttgttcatacggatattttgggtgcagagccactgacggaagttgagccgtataacgtcaactatgatgaaactgttgatgagatcttcgctactgagtgtaagttcccgcaacctaaaggctcagctactgctgtcgagccaattgatatgcagtatatataccgcattacacggccatattccgccattttatataaaacttgcccgcccgaaccgcatagatgggatgccctcggtctgtttattcaacctgtagacgaagattggtgcatagcgcatacaattatgtgctatgtggatgacgttaactcagatcaacaacttcgccatttccagcagacgatttttggccaggacttgatgattcttatcaaccaagttccaaagcgtcttcccctggctgcaagtcgagagagcccagtccgggccgatgtgcttgcaacagcttatcgtcgctggctgcgtgagaaaggtgtgcaatatggtgctctgcgggactaatactagagtcacacaggaaagtactagatgaacaaacttgacgtcaaccagtggtttcctattgctaccactgaagatctcccgaagcgccatgtctttcatgccacgttgttggggcaagaaatggccatctggcgcgatgactctggttcagttaatgcttgggagaaccgctgcccgcatagaggattgcggttgacactgggtgctaataccggtaacgagttgcggtgtcagtatcatggatggacttatgaaagcgggactggtggctgcacttttgtcccagcccatcgcgatgcaccacccccaaatgccgcgcgggttaatacttttcctgtccgcgaaaagcacggctttatctggacgacattaggtcagccgccaggagagcccatttcaatcctcgatgacgctcagcttgtaaacgctgtaaaaacaaatctgcatagcgtagttatagatgctgatattgacggagttgtcagcgtcctacgtcagaatctttcagcgttcatcgatgtgtttggtgcggccagcgctgaagatctgcatttgaaatccatgctgcaagatcgagggattctggtaacaagatcaggctctattgctattcatttttatatgcagcgctcaaccattagtaaatgcgttgtacatgcgcaagtacttactccgggacgtccaggatacgaacttcaaaagaactactcgtatgccatgaacgttatccgcagggcagcagaagctgtagctaccgacttgattagcattacagatatcagcgatcagactatcgaaaagcttgaagtcgttagagaaaacatgactaaggctcctccaacccactatatctgcgaagtggttacgcgtactcaagagacaggtgatattaactcatactggctgaagcctatcggctacccactaccagcattcagtccagggatgcacatcagcatcacaacgccggagggtagcattcgacaatattccctcgtgaacgggcctgacgagcgtgaatccttcatcatcggtgtgaagaaagagattcagtcccgtggcggctccagatcaatgcacgaagatgtgaaggttggaacgcaactaaaagttacacttccgaggaacggttttccactcgtccaaaccagaaaacacccgattctcgtagcaggtggcatcggtatcaccccaattttgtgtatggcacaggctctggatcagcaaggttcatcgtatgaaatacattattttgctcgtgcatttgagcatgttccattccaggatcgactgactgcgttgggcgatcgtttgaatgtgcatcttggcctcggcccagacgagactagagcaaaacttgccgacatcatggagattcataacgcccaagacgtagatgtttacacttgcggcccgcaaccaatgatcgaaactgtatctgctgtcgctcttgctcatggcatcgctgaagagtccatccgatttgaatttttcagtaaaaagaacgatgttcccgtttctgatgaagaatatgaggttgagctcaaaaaaactggtcaaatattcactgtctcgcctggctctacgttgttgcaagcttgtttggacaacgatgttcgtatcgaagcttcttgtgagcagggtgtatgcgggacttgtataactccagtcgtatccggcgatctcgagcatcatgacacttacctttctaagaaagaaagggaaagcggtaagtggatcatgccgtgtgtttcgcgctgcaagtccaaaaaaatcgttctcgatctgtgaggccgggcatcacccacggtatggacgagctgtataaactagagaaagaggagaaatactagatgatcacgctttatgactatgaactctcggggaattgctacaaggtgcggatgctcctttccatcctgaagctaccgtacacgatagagacagtggaatttttcccgtccagggaacataaatcaccggccttcctgcgcatcaatccactcggccagttgccggtattgcgcgatgatgatttcgtgttgcgcgatgcccaggccatcctggtttacctggcgaacaaatacgacaccagtttgacctggtatccgacacatgcgcccgccatcctcggccaggcaaatatgtggctcgcgtttgccgatggtttgaccggctcgatttccgctgcacgtttgcatgatttgtttttttacgatttcaatgcagagcaatgccgtgcccgcgcgcacgaactactgcgcatactggacgaacacctgtgggcggcggaacaaaatggacatcaatggctatgtccggccgccgcacccaccatcgcggatcttgcctgcttcccgtatatcgcattgtcggatgaagccggcgtttcactactcgactacccggcggtacgacgctggctggaccgtgtcaaacgcatccccggattcaccgtcatgtcggggatttttccaacctcagcttccttcgatccggcctcacttgcggaagaaacctgtcaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z