LOCUS BBa_K1627005 4547 bp DNA linear 25-Nov-2024 DEFINITION Device to demethylate theobromine ACCESSION BBa_K1627005 VERSION BBa_K1627005.1 FEATURES Location/Qualifiers promoter 1..35 /label=J23110 RBS 44..58 /label=K082001 CDS 65..1132 /label=ndmB RBS 1141..1155 /label=B0030 CDS 1162..2016 /label=ndmC RBS 2025..2037 /label=B0032 CDS 2044..3810 /label=ndmD RBS 3855..3866 /label=B0034 CDS 3873..4547 /label=gst9 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagtcacaca ggaaagtata 61 ctagatgaaa gaacagctca agccgctgct agaagacaag acttaccttc gccacttctg 121 gcatcccgtg tgtaccctta atgagttcga acgcgccaac gccagtgggc acggccccat 181 gggcgtcacc ttgctaggcg agaaattggt gttggccagg ttaaattcaa agatcattgc 241 ggctgctgac cgatgtgctc atcgatcggc acagctctcc atcggccgcg tttgcagcaa 301 cgccggaaag gactatctcg aatgcccgta tcacggctgg cgctacgatg aggctggggc 361 ctgtcaactg atccctgctt gccctgacaa aagcatctct cctcgggcca agatttcctc 421 attcgattgt gaggtgaaat acgacatcgt gtgggtacgg ctggataaca gcttcgattg 481 cactcagatt ccatacctca gcgatttcga taatcccgac atgcaggtaa tcgttgccga 541 ttcgtatatt tgggagactg ttgccgagcg gcggtgggag aactttacag atttttcgca 601 ctttgccttc gtacacccag ggacgctcta tgatccgttt ttcgctagcc acccaactgt 661 ttacgtgaat cgcgttgatg gtgagttgca attcaaactc gctccgccgc gtgaaatgaa 721 aggcatcccg ccagaagcac cgatgggtga cttcacctac cgctgcacaa tgccgtattc 781 agtaaatctt gaaatcaaat tgtggaaaga tgactcacgt ttcgttcttt ggactaccgc 841 tagccctgtc gataacaagt cttgccggaa ttttatgatt attgtgcgtg agaaggataa 901 tcaacctgat catatgcacc tggctttcca gaagcgggtg cttgacgaag accagcctgt 961 tatcgaatcg caatggcctc tcgaaataca gacctcggaa gtctccgttg caaccgataa 1021 aatttccgtc cagttccgca aatggcataa agagctatct ctgtcagccg ttgaaggacg 1081 ggaggcgttc cgcgattccg tcttgacgaa tgttattgaa gaagaacagt aatactagag 1141 attaaagagg agaaatacta gatgtctact gaccaagtaa tttttaacga ctggcatcca 1201 gtcgctgctt tggaagatgt atctctcgat aaacgatacc gctgtcgact actaggtcgt 1261 acagttagtt atgtgaaaac atctgatgct gttaatgctc attgggaaga aagtgcagat 1321 gaaattaaga ccatccgcgc taaagaaatc tatggtcttc tgtggctctc ctttgccgac 1381 aaacccagtg agatgttcga tattgcagag ttcaaggaac ctgatcgccg aatcgtcagc 1441 gctggatctg tgcgcgtaaa tgtctcagga ctgcgtgcta tcgaaaactt tctggacatg 1501 gctcatttcc cttttgttca tacggatatt ttgggtgcag agccactgac ggaagttgag 1561 ccgtataacg tcaactatga tgaaactgtt gatgagatct tcgctactga gtgtaagttc 1621 ccgcaaccta aaggctcagc tactgctgtc gagccaattg atatgcagta tatataccgc 1681 attacacggc catattccgc cattttatat aaaacttgcc cgcccgaacc gcatagatgg 1741 gatgccctcg gtctgtttat tcaacctgta gacgaagatt ggtgcatagc gcatacaatt 1801 atgtgctatg tggatgacgt taactcagat caacaacttc gccatttcca gcagacgatt 1861 tttggccagg acttgatgat tcttatcaac caagttccaa agcgtcttcc cctggctgca 1921 agtcgagaga gcccagtccg ggccgatgtg cttgcaacag cttatcgtcg ctggctgcgt 1981 gagaaaggtg tgcaatatgg tgctctgcgg gactaatact agagtcacac aggaaagtac 2041 tagatgaaca aacttgacgt caaccagtgg tttcctattg ctaccactga agatctcccg 2101 aagcgccatg tctttcatgc cacgttgttg gggcaagaaa tggccatctg gcgcgatgac 2161 tctggttcag ttaatgcttg ggagaaccgc tgcccgcata gaggattgcg gttgacactg 2221 ggtgctaata ccggtaacga gttgcggtgt cagtatcatg gatggactta tgaaagcggg 2281 actggtggct gcacttttgt cccagcccat cgcgatgcac cacccccaaa tgccgcgcgg 2341 gttaatactt ttcctgtccg cgaaaagcac ggctttatct ggacgacatt aggtcagccg 2401 ccaggagagc ccatttcaat cctcgatgac gctcagcttg taaacgctgt aaaaacaaat 2461 ctgcatagcg tagttataga tgctgatatt gacggagttg tcagcgtcct acgtcagaat 2521 ctttcagcgt tcatcgatgt gtttggtgcg gccagcgctg aagatctgca tttgaaatcc 2581 atgctgcaag atcgagggat tctggtaaca agatcaggct ctattgctat tcatttttat 2641 atgcagcgct caaccattag taaatgcgtt gtacatgcgc aagtacttac tccgggacgt 2701 ccaggatacg aacttcaaaa gaactactcg tatgccatga acgttatccg cagggcagca 2761 gaagctgtag ctaccgactt gattagcatt acagatatca gcgatcagac tatcgaaaag 2821 cttgaagtcg ttagagaaaa catgactaag gctcctccaa cccactatat ctgcgaagtg 2881 gttacgcgta ctcaagagac aggtgatatt aactcatact ggctgaagcc tatcggctac 2941 ccactaccag cattcagtcc agggatgcac atcagcatca caacgccgga gggtagcatt 3001 cgacaatatt ccctcgtgaa cgggcctgac gagcgtgaat ccttcatcat cggtgtgaag 3061 aaagagattc agtcccgtgg cggctccaga tcaatgcacg aagatgtgaa ggttggaacg 3121 caactaaaag ttacacttcc gaggaacggt tttccactcg tccaaaccag aaaacacccg 3181 attctcgtag caggtggcat cggtatcacc ccaattttgt gtatggcaca ggctctggat 3241 cagcaaggtt catcgtatga aatacattat tttgctcgtg catttgagca tgttccattc 3301 caggatcgac tgactgcgtt gggcgatcgt ttgaatgtgc atcttggcct cggcccagac 3361 gagactagag caaaacttgc cgacatcatg gagattcata acgcccaaga cgtagatgtt 3421 tacacttgcg gcccgcaacc aatgatcgaa actgtatctg ctgtcgctct tgctcatggc 3481 atcgctgaag agtccatccg atttgaattt ttcagtaaaa agaacgatgt tcccgtttct 3541 gatgaagaat atgaggttga gctcaaaaaa actggtcaaa tattcactgt ctcgcctggc 3601 tctacgttgt tgcaagcttg tttggacaac gatgttcgta tcgaagcttc ttgtgagcag 3661 ggtgtatgcg ggacttgtat aactccagtc gtatccggcg atctcgagca tcatgacact 3721 tacctttcta agaaagaaag ggaaagcggt aagtggatca tgccgtgtgt ttcgcgctgc 3781 aagtccaaaa aaatcgttct cgatctgtga ggccgggcat cacccacggt atggacgagc 3841 tgtataaact agagaaagag gagaaatact agatgatcac gctttatgac tatgaactct 3901 cggggaattg ctacaaggtg cggatgctcc tttccatcct gaagctaccg tacacgatag 3961 agacagtgga atttttcccg tccagggaac ataaatcacc ggccttcctg cgcatcaatc 4021 cactcggcca gttgccggta ttgcgcgatg atgatttcgt gttgcgcgat gcccaggcca 4081 tcctggttta cctggcgaac aaatacgaca ccagtttgac ctggtatccg acacatgcgc 4141 ccgccatcct cggccaggca aatatgtggc tcgcgtttgc cgatggtttg accggctcga 4201 tttccgctgc acgtttgcat gatttgtttt tttacgattt caatgcagag caatgccgtg 4261 cccgcgcgca cgaactactg cgcatactgg acgaacacct gtgggcggcg gaacaaaatg 4321 gacatcaatg gctatgtccg gccgccgcac ccaccatcgc ggatcttgcc tgcttcccgt 4381 atatcgcatt gtcggatgaa gccggcgttt cactactcga ctacccggcg gtacgacgct 4441 ggctggaccg tgtcaaacgc atccccggat tcaccgtcat gtcggggatt tttccaacct 4501 cagcttcctt cgatccggcc tcacttgcgg aagaaacctg tcaataa //