LOCUS BBa_K1627006 5636 bp DNA linear 22-Dec-2024 DEFINITION Device to demethylate caffeine ACCESSION BBa_K1627006 VERSION BBa_K1627006.1 FEATURES Location/Qualifiers promoter 1..35 /label=J23110 RBS 44..68 /label=K515105 CDS 69..1124 /label=ndmA RBS 1133..1147 /label=K082001 CDS 1154..2221 /label=ndmB RBS 2230..2240 /label=B0030 CDS 2251..3105 /label=ndmC RBS 3114..3126 /label=B0032 CDS 3133..4899 /label=ndmD RBS 4944..4955 /label=B0034 CDS 4962..5636 /label=gst9 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaaatcaa attaaggagg 61 taagataaat ggaacaggca atcattaatg atgaacggga gtatcttcgc catttctggc 121 atcccgtatg tactgtaact gagcttgaaa aggcgcatcc ttccagcctc ggccccctgg 181 ccgttaagct gctgaatgaa cagctcgttg tcgccaagct aggcgatgag tacgtcgcga 241 tgcgtgatag atgcgctcat cgatcagcta agctttcctt gggtacagta agtggaaacc 301 gcctacagtg cccctatcac ggatggcaat atgatacgca tggcgcttgc cagctcgtac 361 cagcgtgccc caacagccca atacccaaca aggctaaagt tgatcgcttc gattgcgaag 421 aacgctatgg attgatttgg attcgattgg actctagttt tgactgcact gaaattccct 481 actttagtgc agccaacgat cctaggttgc gtattgttat acaagaacct tactggtggg 541 atgcgactgc ggaacgtaga tgggaaaatt ttacagattt ttctcacttt gcattcattc 601 acccaggcac gcttttcgat ccaaataatg ctgaacctcc aattgttccg atggatcgat 661 tcaatggtca gtttcggttt gtctacgaca cgccagaaga tatggctgtc ccaaatcagg 721 ctccaattgg ttcattttcg tatacttgca gcatgccgtt tgctattaac cttgaagtat 781 ccaaatactc cagcagttcg ctgcatgtgt tattcaatgt gtcatgtccg gtagacagcc 841 acaccacgaa aaactttctg atcttcgcta gggagcaatc ggacgactcg gattatctgc 901 acattgcatt taatgatctc gtcttcgctg aagacaaacc agtaattgag tcccaatggc 961 ctaaagacgc gccagcagat gaggtctcag tagtcgcaga taaggtatcg atacaatata 1021 gaaaatggct gcgggaacta aaagaagctc ataaagaagg ttcacaagcc ttccgaagtg 1081 ctttgttaga cccagtcatt gaaagcgata ggagctacat ataatactag agtcacacag 1141 gaaagtatac tagatgaaag aacagctcaa gccgctgcta gaagacaaga cttaccttcg 1201 ccacttctgg catcccgtgt gtacccttaa tgagttcgaa cgcgccaacg ccagtgggca 1261 cggccccatg ggcgtcacct tgctaggcga gaaattggtg ttggccaggt taaattcaaa 1321 gatcattgcg gctgctgacc gatgtgctca tcgatcggca cagctctcca tcggccgcgt 1381 ttgcagcaac gccggaaagg actatctcga atgcccgtat cacggctggc gctacgatga 1441 ggctggggcc tgtcaactga tccctgcttg ccctgacaaa agcatctctc ctcgggccaa 1501 gatttcctca ttcgattgtg aggtgaaata cgacatcgtg tgggtacggc tggataacag 1561 cttcgattgc actcagattc catacctcag cgatttcgat aatcccgaca tgcaggtaat 1621 cgttgccgat tcgtatattt gggagactgt tgccgagcgg cggtgggaga actttacaga 1681 tttttcgcac tttgccttcg tacacccagg gacgctctat gatccgtttt tcgctagcca 1741 cccaactgtt tacgtgaatc gcgttgatgg tgagttgcaa ttcaaactcg ctccgccgcg 1801 tgaaatgaaa ggcatcccgc cagaagcacc gatgggtgac ttcacctacc gctgcacaat 1861 gccgtattca gtaaatcttg aaatcaaatt gtggaaagat gactcacgtt tcgttctttg 1921 gactaccgct agccctgtcg ataacaagtc ttgccggaat tttatgatta ttgtgcgtga 1981 gaaggataat caacctgatc atatgcacct ggctttccag aagcgggtgc ttgacgaaga 2041 ccagcctgtt atcgaatcgc aatggcctct cgaaatacag acctcggaag tctccgttgc 2101 aaccgataaa atttccgtcc agttccgcaa atggcataaa gagctatctc tgtcagccgt 2161 tgaaggacgg gaggcgttcc gcgattccgt cttgacgaat gttattgaag aagaacagta 2221 atactagaga ttaaagagga gaaatactag atgtctactg accaagtaat ttttaacgac 2281 tggcatccag tcgctgcttt ggaagatgta tctctcgata aacgataccg ctgtcgacta 2341 ctaggtcgta cagttagtta tgtgaaaaca tctgatgctg ttaatgctca ttgggaagaa 2401 agtgcagatg aaattaagac catccgcgct aaagaaatct atggtcttct gtggctctcc 2461 tttgccgaca aacccagtga gatgttcgat attgcagagt tcaaggaacc tgatcgccga 2521 atcgtcagcg ctggatctgt gcgcgtaaat gtctcaggac tgcgtgctat cgaaaacttt 2581 ctggacatgg ctcatttccc ttttgttcat acggatattt tgggtgcaga gccactgacg 2641 gaagttgagc cgtataacgt caactatgat gaaactgttg atgagatctt cgctactgag 2701 tgtaagttcc cgcaacctaa aggctcagct actgctgtcg agccaattga tatgcagtat 2761 atataccgca ttacacggcc atattccgcc attttatata aaacttgccc gcccgaaccg 2821 catagatggg atgccctcgg tctgtttatt caacctgtag acgaagattg gtgcatagcg 2881 catacaatta tgtgctatgt ggatgacgtt aactcagatc aacaacttcg ccatttccag 2941 cagacgattt ttggccagga cttgatgatt cttatcaacc aagttccaaa gcgtcttccc 3001 ctggctgcaa gtcgagagag cccagtccgg gccgatgtgc ttgcaacagc ttatcgtcgc 3061 tggctgcgtg agaaaggtgt gcaatatggt gctctgcggg actaatacta gagtcacaca 3121 ggaaagtact agatgaacaa acttgacgtc aaccagtggt ttcctattgc taccactgaa 3181 gatctcccga agcgccatgt ctttcatgcc acgttgttgg ggcaagaaat ggccatctgg 3241 cgcgatgact ctggttcagt taatgcttgg gagaaccgct gcccgcatag aggattgcgg 3301 ttgacactgg gtgctaatac cggtaacgag ttgcggtgtc agtatcatgg atggacttat 3361 gaaagcggga ctggtggctg cacttttgtc ccagcccatc gcgatgcacc acccccaaat 3421 gccgcgcggg ttaatacttt tcctgtccgc gaaaagcacg gctttatctg gacgacatta 3481 ggtcagccgc caggagagcc catttcaatc ctcgatgacg ctcagcttgt aaacgctgta 3541 aaaacaaatc tgcatagcgt agttatagat gctgatattg acggagttgt cagcgtccta 3601 cgtcagaatc tttcagcgtt catcgatgtg tttggtgcgg ccagcgctga agatctgcat 3661 ttgaaatcca tgctgcaaga tcgagggatt ctggtaacaa gatcaggctc tattgctatt 3721 catttttata tgcagcgctc aaccattagt aaatgcgttg tacatgcgca agtacttact 3781 ccgggacgtc caggatacga acttcaaaag aactactcgt atgccatgaa cgttatccgc 3841 agggcagcag aagctgtagc taccgacttg attagcatta cagatatcag cgatcagact 3901 atcgaaaagc ttgaagtcgt tagagaaaac atgactaagg ctcctccaac ccactatatc 3961 tgcgaagtgg ttacgcgtac tcaagagaca ggtgatatta actcatactg gctgaagcct 4021 atcggctacc cactaccagc attcagtcca gggatgcaca tcagcatcac aacgccggag 4081 ggtagcattc gacaatattc cctcgtgaac gggcctgacg agcgtgaatc cttcatcatc 4141 ggtgtgaaga aagagattca gtcccgtggc ggctccagat caatgcacga agatgtgaag 4201 gttggaacgc aactaaaagt tacacttccg aggaacggtt ttccactcgt ccaaaccaga 4261 aaacacccga ttctcgtagc aggtggcatc ggtatcaccc caattttgtg tatggcacag 4321 gctctggatc agcaaggttc atcgtatgaa atacattatt ttgctcgtgc atttgagcat 4381 gttccattcc aggatcgact gactgcgttg ggcgatcgtt tgaatgtgca tcttggcctc 4441 ggcccagacg agactagagc aaaacttgcc gacatcatgg agattcataa cgcccaagac 4501 gtagatgttt acacttgcgg cccgcaacca atgatcgaaa ctgtatctgc tgtcgctctt 4561 gctcatggca tcgctgaaga gtccatccga tttgaatttt tcagtaaaaa gaacgatgtt 4621 cccgtttctg atgaagaata tgaggttgag ctcaaaaaaa ctggtcaaat attcactgtc 4681 tcgcctggct ctacgttgtt gcaagcttgt ttggacaacg atgttcgtat cgaagcttct 4741 tgtgagcagg gtgtatgcgg gacttgtata actccagtcg tatccggcga tctcgagcat 4801 catgacactt acctttctaa gaaagaaagg gaaagcggta agtggatcat gccgtgtgtt 4861 tcgcgctgca agtccaaaaa aatcgttctc gatctgtgag gccgggcatc acccacggta 4921 tggacgagct gtataaacta gagaaagagg agaaatacta gatgatcacg ctttatgact 4981 atgaactctc ggggaattgc tacaaggtgc ggatgctcct ttccatcctg aagctaccgt 5041 acacgataga gacagtggaa tttttcccgt ccagggaaca taaatcaccg gccttcctgc 5101 gcatcaatcc actcggccag ttgccggtat tgcgcgatga tgatttcgtg ttgcgcgatg 5161 cccaggccat cctggtttac ctggcgaaca aatacgacac cagtttgacc tggtatccga 5221 cacatgcgcc cgccatcctc ggccaggcaa atatgtggct cgcgtttgcc gatggtttga 5281 ccggctcgat ttccgctgca cgtttgcatg atttgttttt ttacgatttc aatgcagagc 5341 aatgccgtgc ccgcgcgcac gaactactgc gcatactgga cgaacacctg tgggcggcgg 5401 aacaaaatgg acatcaatgg ctatgtccgg ccgccgcacc caccatcgcg gatcttgcct 5461 gcttcccgta tatcgcattg tcggatgaag ccggcgtttc actactcgac tacccggcgg 5521 tacgacgctg gctggaccgt gtcaaacgca tccccggatt caccgtcatg tcggggattt 5581 ttccaacctc agcttccttc gatccggcct cacttgcgga agaaacctgt caataa //