BBa_K1627006 1 BBa_K1627006 Device to demethylate caffeine 2015-09-09T11:00:00Z 2015-09-10T04:37:34Z The ndmA, ndmB, ndmC, and ndmD genes were isolated from Pseudomonas putida CBB5. gst9 was isolated from Janthinobacterium sp. Marseille. This part contains genes that allow for the demethylation of caffeine. The part contains a synthetic operon consisting of ndmA, ndmB, ndmC, and ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110. This part can be used in a ∆guaB strain of E. coli to measure the amount of caffeine in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of caffeine, a growth curve can be made that correlates culture growth with concentration of caffeine. Then if the strain is grown in a minimal media with an unknown concentration of caffeine, the growth can be compared to the growth curve to calculate the initial concentration of caffeine in the media. false false _2044_ 22564 22564 9 false These genes were amplified from the pDCAF3 plasmid (BBa_K734000). A PstI restriction site was removed from the ndmA gene and an EcoRI restriction site was removed from ndmB during the cloning process. The genes were cloned into the pSB1C3 backbone using BioBrick Standard Assembly. false Alejandro Gutierrez annotation2465960 1 ndmA range2465960 1 69 1124 annotation2465961 1 K082001 range2465961 1 1133 1147 annotation2465964 1 ndmC range2465964 1 2251 3105 annotation2465963 1 B0030 range2465963 1 2230 2240 annotation2465967 1 B0034 range2465967 1 4944 4955 annotation2465965 1 B0032 range2465965 1 3114 3126 annotation2465966 1 ndmD range2465966 1 3133 4899 annotation2465968 1 gst9 range2465968 1 4962 5636 annotation2465958 1 J23110 range2465958 1 1 35 annotation2465959 1 K515105 range2465959 1 44 68 annotation2465962 1 ndmB range2465962 1 1154 2221 BBa_K1627006_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagaaatcaaattaaggaggtaagataaatggaacaggcaatcattaatgatgaacgggagtatcttcgccatttctggcatcccgtatgtactgtaactgagcttgaaaaggcgcatccttccagcctcggccccctggccgttaagctgctgaatgaacagctcgttgtcgccaagctaggcgatgagtacgtcgcgatgcgtgatagatgcgctcatcgatcagctaagctttccttgggtacagtaagtggaaaccgcctacagtgcccctatcacggatggcaatatgatacgcatggcgcttgccagctcgtaccagcgtgccccaacagcccaatacccaacaaggctaaagttgatcgcttcgattgcgaagaacgctatggattgatttggattcgattggactctagttttgactgcactgaaattccctactttagtgcagccaacgatcctaggttgcgtattgttatacaagaaccttactggtgggatgcgactgcggaacgtagatgggaaaattttacagatttttctcactttgcattcattcacccaggcacgcttttcgatccaaataatgctgaacctccaattgttccgatggatcgattcaatggtcagtttcggtttgtctacgacacgccagaagatatggctgtcccaaatcaggctccaattggttcattttcgtatacttgcagcatgccgtttgctattaaccttgaagtatccaaatactccagcagttcgctgcatgtgttattcaatgtgtcatgtccggtagacagccacaccacgaaaaactttctgatcttcgctagggagcaatcggacgactcggattatctgcacattgcatttaatgatctcgtcttcgctgaagacaaaccagtaattgagtcccaatggcctaaagacgcgccagcagatgaggtctcagtagtcgcagataaggtatcgatacaatatagaaaatggctgcgggaactaaaagaagctcataaagaaggttcacaagccttccgaagtgctttgttagacccagtcattgaaagcgataggagctacatataatactagagtcacacaggaaagtatactagatgaaagaacagctcaagccgctgctagaagacaagacttaccttcgccacttctggcatcccgtgtgtacccttaatgagttcgaacgcgccaacgccagtgggcacggccccatgggcgtcaccttgctaggcgagaaattggtgttggccaggttaaattcaaagatcattgcggctgctgaccgatgtgctcatcgatcggcacagctctccatcggccgcgtttgcagcaacgccggaaaggactatctcgaatgcccgtatcacggctggcgctacgatgaggctggggcctgtcaactgatccctgcttgccctgacaaaagcatctctcctcgggccaagatttcctcattcgattgtgaggtgaaatacgacatcgtgtgggtacggctggataacagcttcgattgcactcagattccatacctcagcgatttcgataatcccgacatgcaggtaatcgttgccgattcgtatatttgggagactgttgccgagcggcggtgggagaactttacagatttttcgcactttgccttcgtacacccagggacgctctatgatccgtttttcgctagccacccaactgtttacgtgaatcgcgttgatggtgagttgcaattcaaactcgctccgccgcgtgaaatgaaaggcatcccgccagaagcaccgatgggtgacttcacctaccgctgcacaatgccgtattcagtaaatcttgaaatcaaattgtggaaagatgactcacgtttcgttctttggactaccgctagccctgtcgataacaagtcttgccggaattttatgattattgtgcgtgagaaggataatcaacctgatcatatgcacctggctttccagaagcgggtgcttgacgaagaccagcctgttatcgaatcgcaatggcctctcgaaatacagacctcggaagtctccgttgcaaccgataaaatttccgtccagttccgcaaatggcataaagagctatctctgtcagccgttgaaggacgggaggcgttccgcgattccgtcttgacgaatgttattgaagaagaacagtaatactagagattaaagaggagaaatactagatgtctactgaccaagtaatttttaacgactggcatccagtcgctgctttggaagatgtatctctcgataaacgataccgctgtcgactactaggtcgtacagttagttatgtgaaaacatctgatgctgttaatgctcattgggaagaaagtgcagatgaaattaagaccatccgcgctaaagaaatctatggtcttctgtggctctcctttgccgacaaacccagtgagatgttcgatattgcagagttcaaggaacctgatcgccgaatcgtcagcgctggatctgtgcgcgtaaatgtctcaggactgcgtgctatcgaaaactttctggacatggctcatttcccttttgttcatacggatattttgggtgcagagccactgacggaagttgagccgtataacgtcaactatgatgaaactgttgatgagatcttcgctactgagtgtaagttcccgcaacctaaaggctcagctactgctgtcgagccaattgatatgcagtatatataccgcattacacggccatattccgccattttatataaaacttgcccgcccgaaccgcatagatgggatgccctcggtctgtttattcaacctgtagacgaagattggtgcatagcgcatacaattatgtgctatgtggatgacgttaactcagatcaacaacttcgccatttccagcagacgatttttggccaggacttgatgattcttatcaaccaagttccaaagcgtcttcccctggctgcaagtcgagagagcccagtccgggccgatgtgcttgcaacagcttatcgtcgctggctgcgtgagaaaggtgtgcaatatggtgctctgcgggactaatactagagtcacacaggaaagtactagatgaacaaacttgacgtcaaccagtggtttcctattgctaccactgaagatctcccgaagcgccatgtctttcatgccacgttgttggggcaagaaatggccatctggcgcgatgactctggttcagttaatgcttgggagaaccgctgcccgcatagaggattgcggttgacactgggtgctaataccggtaacgagttgcggtgtcagtatcatggatggacttatgaaagcgggactggtggctgcacttttgtcccagcccatcgcgatgcaccacccccaaatgccgcgcgggttaatacttttcctgtccgcgaaaagcacggctttatctggacgacattaggtcagccgccaggagagcccatttcaatcctcgatgacgctcagcttgtaaacgctgtaaaaacaaatctgcatagcgtagttatagatgctgatattgacggagttgtcagcgtcctacgtcagaatctttcagcgttcatcgatgtgtttggtgcggccagcgctgaagatctgcatttgaaatccatgctgcaagatcgagggattctggtaacaagatcaggctctattgctattcatttttatatgcagcgctcaaccattagtaaatgcgttgtacatgcgcaagtacttactccgggacgtccaggatacgaacttcaaaagaactactcgtatgccatgaacgttatccgcagggcagcagaagctgtagctaccgacttgattagcattacagatatcagcgatcagactatcgaaaagcttgaagtcgttagagaaaacatgactaaggctcctccaacccactatatctgcgaagtggttacgcgtactcaagagacaggtgatattaactcatactggctgaagcctatcggctacccactaccagcattcagtccagggatgcacatcagcatcacaacgccggagggtagcattcgacaatattccctcgtgaacgggcctgacgagcgtgaatccttcatcatcggtgtgaagaaagagattcagtcccgtggcggctccagatcaatgcacgaagatgtgaaggttggaacgcaactaaaagttacacttccgaggaacggttttccactcgtccaaaccagaaaacacccgattctcgtagcaggtggcatcggtatcaccccaattttgtgtatggcacaggctctggatcagcaaggttcatcgtatgaaatacattattttgctcgtgcatttgagcatgttccattccaggatcgactgactgcgttgggcgatcgtttgaatgtgcatcttggcctcggcccagacgagactagagcaaaacttgccgacatcatggagattcataacgcccaagacgtagatgtttacacttgcggcccgcaaccaatgatcgaaactgtatctgctgtcgctcttgctcatggcatcgctgaagagtccatccgatttgaatttttcagtaaaaagaacgatgttcccgtttctgatgaagaatatgaggttgagctcaaaaaaactggtcaaatattcactgtctcgcctggctctacgttgttgcaagcttgtttggacaacgatgttcgtatcgaagcttcttgtgagcagggtgtatgcgggacttgtataactccagtcgtatccggcgatctcgagcatcatgacacttacctttctaagaaagaaagggaaagcggtaagtggatcatgccgtgtgtttcgcgctgcaagtccaaaaaaatcgttctcgatctgtgaggccgggcatcacccacggtatggacgagctgtataaactagagaaagaggagaaatactagatgatcacgctttatgactatgaactctcggggaattgc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igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z