BBa_K163000 1 BBa_K163000 T4 phage gene T for self-lysis 2008-08-21T11:00:00Z 2015-05-08T01:10:55Z Sequence from paper "Programmed Escherichia coli Cell Lysis by Expression of Cloned T4 Phage Lysis Genes " by Morita, et al. Biotechnol. Prog., 17 (3), 573 -576, 2001. Gene bank accession number Y00408. Gene t encodes a holin, which is considered to degrade or alter the cytoplasmic membrane, thus allowing lysozymes from T4 gene e to reach the periplasm and gain access to the peptidoglycan layer. Essential component of the cell-lysis system triggered by T4 bacteriophage in E. coli. Expression induces cell-lysis. false false _261_ 0 2866 9 Not in stock false A protein that could effectively trigger cell-lysis in E. coli upon selective induction. When looking for a cell-lysis pathway, system had to be shown to work in E. coli, be non-lethal until induction, and act quickly once triggered. This particular gene was designed by Morita, et al to turn on following inducible expression of a target protein, a feature that could be used to easily purify a protein of interest. Furthermore, Morita, et al report that cell-lysis proceeded efficiently at both mid or late logarithmic growth phase. false Nora Yucel BBa_K163000_sequence 1 gcacctagaatatcattttcgccctctgatattctatttggtgttctggatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataatta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z