LOCUS       BBa_K1631001            1267 bp    DNA     linear       14-May-2024
DEFINITION  rbs - Barnase - rbs - GFP(+LVA) - d.term
ACCESSION   BBa_K1631001
VERSION     BBa_K1631001.1  
FEATURES             Location/Qualifiers
     CDS             16..348
                     /label=Barnase catalitic domain ( I716211 )
     misc_feature    13..15
                     /label=ATG Start Codon
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1..348
                     /label=BBa_K1631000
     misc_feature    361..364
                     /label=conserved
     RBS             357..368
                     /label=BBa_B0034
     misc_feature    375..1094
                     /label=GFP
     misc_feature    1095..1127
                     /label=LVA
     CDS             375..1130
                     /label=BBa_K082003
     stem_loop       1150..1193
                     /label=stem_loop
     misc_feature    1139..1218
                     /label=BBa_B0010
     terminator      1139..1218
                     /label=BBa_B0010
     stem_loop       1234..1253
                     /label=T7 TE
     misc_feature    1260..1260
                     /label=stop
     polyA_site      1254..1267
                     /label=polya
     misc_feature    1227..1267
                     /label=BBa_B0012
     terminator      1227..1267
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aaatggcaca ggttatcaac acgtttgacg gggttgcgga ttatcttcag
       61 acatatcata agctacctga taattacatt acaaaatcag aagcacaagc cctcggctgg
      121 gtggcatcaa aagggaacct tgcagacgtc gctccgggga aaagcatcgg cggagacatc
      181 ttctcaaaca gggaaggcaa actcccgggc aaaagcggac gaacatggcg tgaagcggat
      241 attaactata catcaggctt cagaaattca gaccggattc tttactcaag cgactggctg
      301 atttacaaaa caacggacca ttatcagacc tttacaaaaa tcagataata ctagagaaag
      361 aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      421 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      481 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
      541 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag
      601 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
      661 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
      721 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
      781 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
      841 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
      901 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      961 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     1021 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     1081 tatacaaaag gcctgctgca aacgacgaaa actacgcttt agtagcttaa tactagagcc
     1141 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1201 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1261 gtttata
//