LOCUS       BBa_K1631009            2247 bp    DNA     linear       22-Dec-2024
DEFINITION  rbs - Colicin Lysis prrotein - rbs -ColicinE3 - rbs - Colicin 
            Immunity protein - d.term
ACCESSION   BBa_K1631009
VERSION     BBa_K1631009.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=BBa_B0034
     CDS             13..156
                     /label=Colicin Lysis Protein 
     misc_feature    1..156
                     /label=BBa_K1631003
     RBS             165..176
                     /label=BBa_B0034
     CDS             177..1832
                     /label=Colicin-E3
     misc_feature    165..1832
                     /label=BBa_K1631002
     RBS             1841..1852
                     /label=BBa_B0034
     CDS             1853..2110
                     /label=Colicin Immunity protein
     misc_feature    1841..2110
                     /label=BBa_K1631004
     stem_loop       2130..2173
                     /label=stem_loop
     misc_feature    2119..2198
                     /label=BBa_B0010
     terminator      2119..2198
                     /label=BBa_B0010
     stem_loop       2214..2233
                     /label=T7 TE
     misc_feature    2240..2240
                     /label=stop
     polyA_site      2234..2247
                     /label=polya
     misc_feature    2207..2247
                     /label=BBa_B0012
     terminator      2207..2247
                     /label=BBa_B0012
     terminator      2119..2247
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aaatgaaaaa aatcaccggt atcatcctac tactgctggc agttatcatc
       61 ctgtctgcat gccaggcgaa ctacatccgt gacgttcagg gtggtacagt ttctccatct
      121 tctaccgcgg aagttaccgg tctagcaact cagtaatact agagaaagag gagaaaatgt
      181 ctggcggtga cggtcgtggt cacaatactg gtgctcattc tacatctggc aacatcaacg
      241 gcggtcctac tggtcttggt gttggtggcg gtgcatctga cggttctggc tggtcttccg
      301 agaacaatcc atggggtggc ggttctggtt ctggtattca ctggggcggt ggctctggtc
      361 atggtaacgg tggcggtaat ggtaactccg gtggcggttc tggtaccggt ggtaacctgt
      421 ctgcggttgc agctccagtt gcattcggtt tcccagcact atctactcct ggtgcaggtg
      481 gtctagcagt ttctatctca gcaggtgcac tgtcagcagc aatcgcggat atcatggcag
      541 cgctgaaagg tccattcaaa ttcggtctgt ggggtgttgc gctgtacggt gttctgccgt
      601 ctcagatcgc gaaagacgac ccgaacatga tgtctaaaat cgttacatct ctgccggcgg
      661 acgacatcac cgaatctccg gtttcttctc tgccgctgga caaggctacc gttaatgtca
      721 acgtacgtgt agttgatgac gttaaagacg aacgtcagaa catatctgta gtttctggtg
      781 ttccgatgtc tgttccagtc gttgatgcta agcctactga gcgtccaggc gtattcactg
      841 cgtctatacc tggcgctcca gtactgaaca tctccgtcaa taactctact ccagcggttc
      901 agactctatc tccaggcgtt actaataaca ccgacaaaga cgtacgacca gcaggcttca
      961 ctcaaggagg taatacacgt gacgcggtta tccgattccc gaaagactcc ggtcataatg
     1021 cggtctacgt atctgtatct gacgtactgt ctccggatca ggtcaagcag cgtcaggacg
     1081 aggaaaaccg ccgtcaacag gagtgggacg caactcatcc agttgaagca gctgagcgta
     1141 actacgagcg tgcacgtgct gagctgaatc aggcgaacga ggacgttgca cgtaatcaag
     1201 agcgtcaggc gaaagcggta caggtctaca attctcgtaa gtccgaactg gacgcagcta
     1261 acaagacact tgcagacgca atcgcggaaa tcaaacagtt caaccgtttc gcacatgacc
     1321 ctatggcagg tggtcaccgt atgtggcaga tggcaggtct gaaagcacag cgtgcacaga
     1381 ccgacgttaa caacaaacaa gcagcgttcg acgcggcggc gaaagaaaaa tctgacgcag
     1441 acgcagcact gtcttctgcg atggaatctc gtaaaaaaaa agaagacaaa aaacgttctg
     1501 cggaaaacaa cctgaacgac gaaaaaaaca aaccgcgtaa aggtttcaaa gactacggtc
     1561 acgactacca tccagctccg aagactgaga acatcaaagg tctaggcgac ctgaaaccag
     1621 gtatcccgaa aaccccgaaa cagaacggtg gtggtaaacg taaacgttgg accggtgaca
     1681 aaggtcgtaa gatctacgag tgggattctc agcacggtga gctagaaggc tatcgtgcat
     1741 ctgacggtca gcacctgggt tctttcgacc caaaaaccgg taaccagctg aaaggtccag
     1801 acccgaaacg taacatcaaa aaatacctgt aatactagag aaagaggaga aaatgggtct
     1861 gaaactggac ctgacctggt tcgacaaatc taccgaagac ttcaaaggtg aagaatactc
     1921 taaagacttc ggtgacgacg gttctgttat ggaatctctg ggtgttccgt tcaaagacaa
     1981 cgttaacaac ggttgcttcg acgttatcgc ggaatgggtt ccactactac agccgtactt
     2041 caaccaccag atcgacatct ctgacaacga atacttcgtt tctttcgact accgtgacgg
     2101 tgactggtaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     2161 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     2221 cttcgggtgg gcctttctgc gtttata
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