BBa_K163101
1
BBa_K163101
pUC8 Plasmid DNA
2008-10-10T11:00:00Z
2015-05-08T01:10:55Z
Vieira, J., and J. Messing. 1982. The pUC plasmids, an M13mp7-derived
system for insertion mutagenesis and sequencing with synthetic universal
primers. Gene 19:259???268.
pUC8 is a plasmid that has the last 336 AA of RsaA (Caulobacter surface protein) and it allows for a cloning of desired segment into a EcoR1, Sma1 and or BamH1 site upstream of the 336.
false
false
_261_
0
2746
9
Not in stock
false
This plasmid is used for the Caulobacter secretion system. We use this as the main template in which we splice in our BioBricks for Caulobacter.
false
Daniel M. Choi
annotation1980426
1
EcoRI
range1980426
1
2016
2021
BBa_K163101_sequence
1
ggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaacctcaacttttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaattacgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagactctgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctggggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgaattcccggggatccgtcgctgacccggccttcggcggcttcgaaaccctccgcgtcgctggcgcggcggctcaaggctcgcacaacgccaacggcttcacggctctgcaactgggcgcgacggcgggtgcgacgaccttcaccaacgttgcggtgaatgtcggcctgaccgttctggcggctccgaccggtacgacgaccgtgaccctggccaacgccacgggcacctcggacgtgttcaacctgaccctgtcgtcctcggccgctctggccgctggtacggttgcgctggctggcgtcgagacggtgaacatcgccgccaccgacaccaacacgaccgctcacgtcgacacgctgacgctgcaagccacctcggccaagtcgatcgtggtgacgggcaacgccggtctgaacctgaccaacaccggcaacacggctgtcaccagcttcgacgccagcgccgtcaccggcacgggctcggctgtgaccttcgtgtcggccaacaccacggtgggtgaagtcgtcacgatccgcggcggcgctggcgccgactcgctgaccggttcggccaccgccaatgacaccatcatcggtggcgctggcgctgacaccctggtctacaccggcggtacggacaccttcacgggtggcacgggcgcggatatcttcgatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaagcgaacgtctgatcctcgcctaggcgaggatcgctagactaagagaccccgtcttccgaaagggaggcggggtctttcttatgggcgctacgcgctggccggccttgcctagttccggtggctatgatttagcgggactggggggcttgctcactttccgccacaatttcgtggtcgagacggcgccttagttgttactgtacatggccgcgtcggttcgcgcggcgtcctgaaggctcacaatgttcaagcgcagcggcgcgaagccgacgatcctcgaccaggccgtgctggtcgcccgcccggcggtgatcaccgccatggtcttcagcttcttcatcaacattctggccctggtcagcccgctgtacatgcggcatgcaagcttggtaccctcagccgaaatgcctgccgttgctagacattgccagccagtgcccgtcactcccgtactaactgtcacgaacccctgcaataactgtcacgcccccctgcaataactgtcacgaacccctgcaataactgtcacgcccccaaacctgcaaacccagcaggggcgggggctggcggggtgttggaaaaatccatccatgattatctaagaataatccactaggcgcggttatcagcgcccttgtggggcgctgctgcccttgcccaatatgcccggccagaggccggatagctggtctattcgctgcgctaggctacacaccgccccaccgctgcgcggcagggggaaaggcgggcaaagcccgctaaaccccacaccaaaccccgcagaaatacgctggagcgcttttagccgctttagcggcctttccccctacccgaagggtgggggcgcgtgtgcagccccgcagggcctgtctcggtcgatcattcagcccggctcataagccgaatt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z