LOCUS       BBa_K1631012            1330 bp    DNA     linear       22-Nov-2024
DEFINITION  Plac -rbs- Barnase -rbs- GFP -d.term
ACCESSION   BBa_K1631012
VERSION     BBa_K1631012.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     CDS             79..411
                     /label=Barnase catalitic domain ( I716211 )
     misc_feature    76..78
                     /label=ATG Start Codon
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    64..411
                     /label=BBa_K1631000
     misc_feature    424..427
                     /label=conserved
     RBS             420..431
                     /label=BBa_B0034
     misc_feature    438..1157
                     /label=GFP
     misc_feature    1158..1190
                     /label=LVA
     CDS             438..1193
                     /label=BBa_K082003
     stem_loop       1213..1256
                     /label=stem_loop
     misc_feature    1202..1281
                     /label=BBa_B0010
     terminator      1202..1281
                     /label=BBa_B0010
     stem_loop       1297..1316
                     /label=T7 TE
     misc_feature    1323..1323
                     /label=stop
     polyA_site      1317..1330
                     /label=polya
     misc_feature    1290..1330
                     /label=BBa_B0012
     terminator      1290..1330
                     /label=BBa_B0012
     terminator      1202..1330
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaaatggc acaggttatc aacacgtttg acggggttgc ggattatctt
      121 cagacatatc ataagctacc tgataattac attacaaaat cagaagcaca agccctcggc
      181 tgggtggcat caaaagggaa ccttgcagac gtcgctccgg ggaaaagcat cggcggagac
      241 atcttctcaa acagggaagg caaactcccg ggcaaaagcg gacgaacatg gcgtgaagcg
      301 gatattaact atacatcagg cttcagaaat tcagaccgga ttctttactc aagcgactgg
      361 ctgatttaca aaacaacgga ccattatcag acctttacaa aaatcagata atactagaga
      421 aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
      481 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      541 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      601 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
      661 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      721 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      781 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      841 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      901 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
      961 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1021 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     1081 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     1141 aactatacaa aaggcctgct gcaaacgacg aaaactacgc tttagtagct taatactaga
     1201 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1261 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1321 tgcgtttata
//