LOCUS       BBa_K1631013            2126 bp    DNA     linear       29-Apr-2024
DEFINITION  Pconst(Medium) - rbs - Colcin E3 - rbs - Colicin Immunity protein 
            - dterm
ACCESSION   BBa_K1631013
VERSION     BBa_K1631013.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     RBS             44..55
                     /label=BBa_B0034
     CDS             56..1711
                     /label=Colicin-E3
     misc_feature    44..1711
                     /label=BBa_K1631002
     RBS             1720..1731
                     /label=BBa_B0034
     CDS             1732..1989
                     /label=Colicin Immunity protein
     misc_feature    1720..1989
                     /label=BBa_K1631004
     stem_loop       2009..2052
                     /label=stem_loop
     misc_feature    1998..2077
                     /label=BBa_B0010
     terminator      1998..2077
                     /label=BBa_B0010
     stem_loop       2093..2112
                     /label=T7 TE
     misc_feature    2119..2119
                     /label=stop
     polyA_site      2113..2126
                     /label=polya
     misc_feature    2086..2126
                     /label=BBa_B0012
     terminator      2086..2126
                     /label=BBa_B0012
     terminator      1998..2126
                     /label=BBa_B0015
     misc_feature    44..2126
                     /label=BBa_K1631008
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaaatgtc
       61 tggcggtgac ggtcgtggtc acaatactgg tgctcattct acatctggca acatcaacgg
      121 cggtcctact ggtcttggtg ttggtggcgg tgcatctgac ggttctggct ggtcttccga
      181 gaacaatcca tggggtggcg gttctggttc tggtattcac tggggcggtg gctctggtca
      241 tggtaacggt ggcggtaatg gtaactccgg tggcggttct ggtaccggtg gtaacctgtc
      301 tgcggttgca gctccagttg cattcggttt cccagcacta tctactcctg gtgcaggtgg
      361 tctagcagtt tctatctcag caggtgcact gtcagcagca atcgcggata tcatggcagc
      421 gctgaaaggt ccattcaaat tcggtctgtg gggtgttgcg ctgtacggtg ttctgccgtc
      481 tcagatcgcg aaagacgacc cgaacatgat gtctaaaatc gttacatctc tgccggcgga
      541 cgacatcacc gaatctccgg tttcttctct gccgctggac aaggctaccg ttaatgtcaa
      601 cgtacgtgta gttgatgacg ttaaagacga acgtcagaac atatctgtag tttctggtgt
      661 tccgatgtct gttccagtcg ttgatgctaa gcctactgag cgtccaggcg tattcactgc
      721 gtctatacct ggcgctccag tactgaacat ctccgtcaat aactctactc cagcggttca
      781 gactctatct ccaggcgtta ctaataacac cgacaaagac gtacgaccag caggcttcac
      841 tcaaggaggt aatacacgtg acgcggttat ccgattcccg aaagactccg gtcataatgc
      901 ggtctacgta tctgtatctg acgtactgtc tccggatcag gtcaagcagc gtcaggacga
      961 ggaaaaccgc cgtcaacagg agtgggacgc aactcatcca gttgaagcag ctgagcgtaa
     1021 ctacgagcgt gcacgtgctg agctgaatca ggcgaacgag gacgttgcac gtaatcaaga
     1081 gcgtcaggcg aaagcggtac aggtctacaa ttctcgtaag tccgaactgg acgcagctaa
     1141 caagacactt gcagacgcaa tcgcggaaat caaacagttc aaccgtttcg cacatgaccc
     1201 tatggcaggt ggtcaccgta tgtggcagat ggcaggtctg aaagcacagc gtgcacagac
     1261 cgacgttaac aacaaacaag cagcgttcga cgcggcggcg aaagaaaaat ctgacgcaga
     1321 cgcagcactg tcttctgcga tggaatctcg taaaaaaaaa gaagacaaaa aacgttctgc
     1381 ggaaaacaac ctgaacgacg aaaaaaacaa accgcgtaaa ggtttcaaag actacggtca
     1441 cgactaccat ccagctccga agactgagaa catcaaaggt ctaggcgacc tgaaaccagg
     1501 tatcccgaaa accccgaaac agaacggtgg tggtaaacgt aaacgttgga ccggtgacaa
     1561 aggtcgtaag atctacgagt gggattctca gcacggtgag ctagaaggct atcgtgcatc
     1621 tgacggtcag cacctgggtt ctttcgaccc aaaaaccggt aaccagctga aaggtccaga
     1681 cccgaaacgt aacatcaaaa aatacctgta atactagaga aagaggagaa aatgggtctg
     1741 aaactggacc tgacctggtt cgacaaatct accgaagact tcaaaggtga agaatactct
     1801 aaagacttcg gtgacgacgg ttctgttatg gaatctctgg gtgttccgtt caaagacaac
     1861 gttaacaacg gttgcttcga cgttatcgcg gaatgggttc cactactaca gccgtacttc
     1921 aaccaccaga tcgacatctc tgacaacgaa tacttcgttt ctttcgacta ccgtgacggt
     1981 gactggtaat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2041 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2101 ttcgggtggg cctttctgcg tttata
//