BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1631008 1 BBa_K1631008 rbs - ColicinE3 - rbs - Colicin Immunity protein - d.term 2015-09-13T11:00:00Z 2015-09-14T07:16:09Z Assembly When this is placed under a promoter, Colicin-E3(BBa_K1631002) is co-expressed with its immunity protein(BBa_K1631004). false false _2048_ 23567 23567 9 false none false Yuto Yamanaka component2455444 1 BBa_B0015 component2455437 1 BBa_K1631004 component2455434 1 BBa_K1631002 annotation2455437 1 BBa_K1631004 range2455437 1 1677 1946 annotation2455444 1 BBa_B0015 range2455444 1 1955 2083 annotation2455434 1 BBa_K1631002 range2455434 1 1 1668 BBa_K1631014 1 BBa_K1631014 Pconst(strong) -rbs- Colicin E3 -rbs- Colicin Immunity protein -d.tem 2015-09-13T11:00:00Z 2015-09-14T07:59:58Z Assembly Colicin-E3 and its immunity protein is co-expressed under BBa_J23101 false false _2048_ 23567 23567 9 false none false Yuto Yamanaka component2455555 1 BBa_K1631008 component2455541 1 BBa_J23101 annotation2455555 1 BBa_K1631008 range2455555 1 44 2126 annotation2455541 1 BBa_J23101 range2455541 1 1 35 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1631004 1 BBa_K1631004 Tlanslational unit of Immunity protein for Colicin-E3 2015-09-12T11:00:00Z 2015-09-13T12:52:31Z pColE3-CA38 Immunity protein for Colicin-E3 binds in between the Translocation domain and Ctalitic domain. Curiously, immunity protein does not block the active site.[1] It is considered that immunity protein blocks access of this enzyme to the ribosome. Colicins are a cytotoxins which are released to environment and kill other related strains. false false _2048_ 23567 23567 9 false none false Yuto Yamanaka annotation2453060 1 BBa_B0034 range2453060 1 1 12 annotation2453061 1 Colicin Immunity protein range2453061 1 13 270 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K1631002 1 BBa_K1631002 Translational unit of Colicin-E3 2015-09-11T11:00:00Z 2015-09-14T03:43:50Z pColE3-CA38 Colicin-E3 cleave 16SrRNA at a specific site. This activity is inhibited by immunity protein for Colicin-E3(BBa_). When lysis protein for Colicin-E3(BBa_) is co-expressed, Colicin-E3 is emited to environment and kill other strain. Colicin is a type of bacteriocin produced by Escherichia coli. false false _2048_ 23567 23567 9 false none false Yuto Yamanaka annotation2452986 1 BBa_B0034 range2452986 1 1 12 annotation2452987 1 Colicin-E3 range2452987 1 13 1668 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1631008_sequence 1 aaagaggagaaaatgtctggcggtgacggtcgtggtcacaatactggtgctcattctacatctggcaacatcaacggcggtcctactggtcttggtgttggtggcggtgcatctgacggttctggctggtcttccgagaacaatccatggggtggcggttctggttctggtattcactggggcggtggctctggtcatggtaacggtggcggtaatggtaactccggtggcggttctggtaccggtggtaacctgtctgcggttgcagctccagttgcattcggtttcccagcactatctactcctggtgcaggtggtctagcagtttctatctcagcaggtgcactgtcagcagcaatcgcggatatcatggcagcgctgaaaggtccattcaaattcggtctgtggggtgttgcgctgtacggtgttctgccgtctcagatcgcgaaagacgacccgaacatgatgtctaaaatcgttacatctctgccggcggacgacatcaccgaatctccggtttcttctctgccgctggacaaggctaccgttaatgtcaacgtacgtgtagttgatgacgttaaagacgaacgtcagaacatatctgtagtttctggtgttccgatgtctgttccagtcgttgatgctaagcctactgagcgtccaggcgtattcactgcgtctatacctggcgctccagtactgaacatctccgtcaataactctactccagcggttcagactctatctccaggcgttactaataacaccgacaaagacgtacgaccagcaggcttcactcaaggaggtaatacacgtgacgcggttatccgattcccgaaagactccggtcataatgcggtctacgtatctgtatctgacgtactgtctccggatcaggtcaagcagcgtcaggacgaggaaaaccgccgtcaacaggagtgggacgcaactcatccagttgaagcagctgagcgtaactacgagcgtgcacgtgctgagctgaatcaggcgaacgaggacgttgcacgtaatcaagagcgtcaggcgaaagcggtacaggtctacaattctcgtaagtccgaactggacgcagctaacaagacacttgcagacgcaatcgcggaaatcaaacagttcaaccgtttcgcacatgaccctatggcaggtggtcaccgtatgtggcagatggcaggtctgaaagcacagcgtgcacagaccgacgttaacaacaaacaagcagcgttcgacgcggcggcgaaagaaaaatctgacgcagacgcagcactgtcttctgcgatggaatctcgtaaaaaaaaagaagacaaaaaacgttctgcggaaaacaacctgaacgacgaaaaaaacaaaccgcgtaaaggtttcaaagactacggtcacgactaccatccagctccgaagactgagaacatcaaaggtctaggcgacctgaaaccaggtatcccgaaaaccccgaaacagaacggtggtggtaaacgtaaacgttggaccggtgacaaaggtcgtaagatctacgagtgggattctcagcacggtgagctagaaggctatcgtgcatctgacggtcagcacctgggttctttcgacccaaaaaccggtaaccagctgaaaggtccagacccgaaacgtaacatcaaaaaatacctgtaatactagagaaagaggagaaaatgggtctgaaactggacctgacctggttcgacaaatctaccgaagacttcaaaggtgaagaatactctaaagacttcggtgacgacggttctgttatggaatctctgggtgttccgttcaaagacaacgttaacaacggttgcttcgacgttatcgcggaatgggttccactactacagccgtacttcaaccaccagatcgacatctctgacaacgaatacttcgtttctttcgactaccgtgacggtgactggtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1631002_sequence 1 aaagaggagaaaatgtctggcggtgacggtcgtggtcacaatactggtgctcattctacatctggcaacatcaacggcggtcctactggtcttggtgttggtggcggtgcatctgacggttctggctggtcttccgagaacaatccatggggtggcggttctggttctggtattcactggggcggtggctctggtcatggtaacggtggcggtaatggtaactccggtggcggttctggtaccggtggtaacctgtctgcggttgcagctccagttgcattcggtttcccagcactatctactcctggtgcaggtggtctagcagtttctatctcagcaggtgcactgtcagcagcaatcgcggatatcatggcagcgctgaaaggtccattcaaattcggtctgtggggtgttgcgctgtacggtgttctgccgtctcagatcgcgaaagacgacccgaacatgatgtctaaaatcgttacatctctgccggcggacgacatcaccgaatctccggtttcttctctgccgctggacaaggctaccgttaatgtcaacgtacgtgtagttgatgacgttaaagacgaacgtcagaacatatctgtagtttctggtgttccgatgtctgttccagtcgttgatgctaagcctactgagcgtccaggcgtattcactgcgtctatacctggcgctccagtactgaacatctccgtcaataactctactccagcggttcagactctatctccaggcgttactaataacaccgacaaagacgtacgaccagcaggcttcactcaaggaggtaatacacgtgacgcggttatccgattcccgaaagactccggtcataatgcggtctacgtatctgtatctgacgtactgtctccggatcaggtcaagcagcgtcaggacgaggaaaaccgccgtcaacaggagtgggacgcaactcatccagttgaagcagctgagcgtaactacgagcgtgcacgtgctgagctgaatcaggcgaacgaggacgttgcacgtaatcaagagcgtcaggcgaaagcggtacaggtctacaattctcgtaagtccgaactggacgcagctaacaagacacttgcagacgcaatcgcggaaatcaaacagttcaaccgtttcgcacatgaccctatggcaggtggtcaccgtatgtggcagatggcaggtctgaaagcacagcgtgcacagaccgacgttaacaacaaacaagcagcgttcgacgcggcggcgaaagaaaaatctgacgcagacgcagcactgtcttctgcgatggaatctcgtaaaaaaaaagaagacaaaaaacgttctgcggaaaacaacctgaacgacgaaaaaaacaaaccgcgtaaaggtttcaaagactacggtcacgactaccatccagctccgaagactgagaacatcaaaggtctaggcgacctgaaaccaggtatcccgaaaaccccgaaacagaacggtggtggtaaacgtaaacgttggaccggtgacaaaggtcgtaagatctacgagtgggattctcagcacggtgagctagaaggctatcgtgcatctgacggtcagcacctgggttctttcgacccaaaaaccggtaaccagctgaaaggtccagacccgaaacgtaacatcaaaaaatacctgtaa BBa_K1631004_sequence 1 aaagaggagaaaatgggtctgaaactggacctgacctggttcgacaaatctaccgaagacttcaaaggtgaagaatactctaaagacttcggtgacgacggttctgttatggaatctctgggtgttccgttcaaagacaacgttaacaacggttgcttcgacgttatcgcggaatgggttccactactacagccgtacttcaaccaccagatcgacatctctgacaacgaatacttcgtttctttcgactaccgtgacggtgactggtaa BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_K1631014_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaaatgtctggcggtgacggtcgtggtcacaatactggtgctcattctacatctggcaacatcaacggcggtcctactggtcttggtgttggtggcggtgcatctgacggttctggctggtcttccgagaacaatccatggggtggcggttctggttctggtattcactggggcggtggctctggtcatggtaacggtggcggtaatggtaactccggtggcggttctggtaccggtggtaacctgtctgcggttgcagctccagttgcattcggtttcccagcactatctactcctggtgcaggtggtctagcagtttctatctcagcaggtgcactgtcagcagcaatcgcggatatcatggcagcgctgaaaggtccattcaaattcggtctgtggggtgttgcgctgtacggtgttctgccgtctcagatcgcgaaagacgacccgaacatgatgtctaaaatcgttacatctctgccggcggacgacatcaccgaatctccggtttcttctctgccgctggacaaggctaccgttaatgtcaacgtacgtgtagttgatgacgttaaagacgaacgtcagaacatatctgtagtttctggtgttccgatgtctgttccagtcgttgatgctaagcctactgagcgtccaggcgtattcactgcgtctatacctggcgctccagtactgaacatctccgtcaataactctactccagcggttcagactctatctccaggcgttactaataacaccgacaaagacgtacgaccagcaggcttcactcaaggaggtaatacacgtgacgcggttatccgattcccgaaagactccggtcataatgcggtctacgtatctgtatctgacgtactgtctccggatcaggtcaagcagcgtcaggacgaggaaaaccgccgtcaacaggagtgggacgcaactcatccagttgaagcagctgagcgtaactacgagcgtgcacgtgctgagctgaatcaggcgaacgaggacgttgcacgtaatcaagagcgtcaggcgaaagcggtacaggtctacaattctcgtaagtccgaactggacgcagctaacaagacacttgcagacgcaatcgcggaaatcaaacagttcaaccgtttcgcacatgaccctatggcaggtggtcaccgtatgtggcagatggcaggtctgaaagcacagcgtgcacagaccgacgttaacaacaaacaagcagcgttcgacgcggcggcgaaagaaaaatctgacgcagacgcagcactgtcttctgcgatggaatctcgtaaaaaaaaagaagacaaaaaacgttctgcggaaaacaacctgaacgacgaaaaaaacaaaccgcgtaaaggtttcaaagactacggtcacgactaccatccagctccgaagactgagaacatcaaaggtctaggcgacctgaaaccaggtatcccgaaaaccccgaaacagaacggtggtggtaaacgtaaacgttggaccggtgacaaaggtcgtaagatctacgagtgggattctcagcacggtgagctagaaggctatcgtgcatctgacggtcagcacctgggttctttcgacccaaaaaccggtaaccagctgaaaggtccagacccgaaacgtaacatcaaaaaatacctgtaatactagagaaagaggagaaaatgggtctgaaactggacctgacctggttcgacaaatctaccgaagacttcaaaggtgaagaatactctaaagacttcggtgacgacggttctgttatggaatctctgggtgttccgttcaaagacaacgttaacaacggttgcttcgacgttatcgcggaatgggttccactactacagccgtacttcaaccaccagatcgacatctctgacaacgaatacttcgtttctttcgactaccgtgacggtgactggtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z