LOCUS       BBa_K1631018             823 bp    DNA     linear       06-May-2024
DEFINITION  Pconst(Strong) -rbs- CheZ -d.term
ACCESSION   BBa_K1631018
VERSION     BBa_K1631018.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     primer_bind     42..61
                     /label=fwd primer
     misc_feature    42..44
                     /label=start
     primer_bind     665..686
                     /label=rev primer
     misc_feature    684..686
                     /label=terminator
     CDS             42..686
                     /label=CheZ
     misc_feature    684..686
                     /label=TGA to TAA
     CDS             42..686
                     /label=BBa_K629003
     stem_loop       706..749
                     /label=stem_loop
     misc_feature    695..774
                     /label=BBa_B0010
     terminator      695..774
                     /label=BBa_B0010
     stem_loop       790..809
                     /label=T7 TE
     misc_feature    816..816
                     /label=stop
     polyA_site      810..823
                     /label=polya
     misc_feature    783..823
                     /label=BBa_B0012
     terminator      783..823
                     /label=BBa_B0012
     terminator      695..823
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gatgatgcaa ccatcaatca
       61 aacctgctga cgagcattca gctggcgata tcattgcgcg catcggcagc ctgacgcgta
      121 tgctgcgcga cagtttgcgg gaactggggc tggatcaggc cattgccgaa gcggcggaag
      181 ccatccccga tgcgcgcgat cgtttgtact atgttgtgca gatgaccgcc caggctgcgg
      241 agcgggcgct gaacagtgtt gaggcgtcac aaccgcatca ggatcaaatg gagaaatcag
      301 caaaagcgtt aacccaacgt tgggatgact ggtttgccga tccgattgac cttgccgacg
      361 cccgtgaact ggtaacagat acacgacaat ttctggcaga tgtacccgcg cataccagct
      421 ttactaacgc gcaactgctg gaaatcatga tggcgcagga ttttcaggat ctcaccgggc
      481 aggtcattaa gcggatgatg gatgtcattc aggagatcga acgccagttg ctgatggtgc
      541 tgttggaaaa catcccggaa caggagtcgc gtccaaaacg tgaaaaccag agtttgctta
      601 atggacctca ggtcgatacc agcaaagccg gtgtggtagc cagtcaggat caggtggacg
      661 atttgttgga tagtcttgga ttttaatact agagccaggc atcaaataaa acgaaaggct
      721 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      781 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//