BBa_K1631022
type: ComponentDefinition
persistent identity: BBa K1631022
display id: BBa_K1631022
version: 1
was generated by: iGEM to SBOL conversion
description: Plux -rbs- Barnase -rbs- GFP -d.term
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:26:39Z
mutable provenance: Assembly
owned by: james
owned by: myers
mutable description: Barnase and GFP is co-expressed by luxR/AHL complex.
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Yuto Yamanaka
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K1631001
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_K1631001
sequence: https://synbiohub.org/public/igem/BBa_K1631022_sequence/1

BBa_K1631001
type: Component
persistent identity: component2455743
display id: component2455743
version: 1
top level: BBa_K1631022
definition: BBa_K1631001
access: public

BBa_R0062
type: Component
persistent identity: component2455723
display id: component2455723
version: 1
top level: BBa_K1631022
definition: lux pR
access: public

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2455723
display id: annotation2455723
version: 1
top level: BBa_K1631022
location: https://synbiohub.org/public/igem/BBa_K1631022/annotation2455723/range2455723/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1631022/annotation2455723/range2455723/1
type: Range
persistent identity: range2455723
display id: range2455723
version: 1
top level: BBa_K1631022
direction: direction/forward
start: 1
end: 55
orientation: inline

BBa_K1631001
type: SequenceAnnotation
persistent identity: annotation2455743
display id: annotation2455743
version: 1
top level: BBa_K1631022
location: https://synbiohub.org/public/igem/BBa_K1631022/annotation2455743/range2455743/1
component: BBa_K1631001

https://synbiohub.org/public/igem/BBa_K1631022/annotation2455743/range2455743/1
type: Range
persistent identity: range2455743
display id: range2455743
version: 1
top level: BBa_K1631022
direction: direction/reverse
start: 64
end: 1330
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_K1631001
type: ComponentDefinition
persistent identity: BBa K1631001
display id: BBa_K1631001
version: 1
was generated by: iGEM to SBOL conversion
description: rbs - Barnase - rbs - GFP(+LVA) - d.term
created: 2015-09-11T11:00:00Z
modified: 2015-09-14T07:30:24Z
mutable provenance: Assembly
owned by: james
owned by: myers
mutable description: This part is a composite part of RBS(BBa_B0034), Barnase(BBa_K1631000), RBS(BBa_B0034), GFP(+LVA)(BBa_K082003), double termnator (BBa_B0015).
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Yuto Yamanaka
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1631000
component: BBa_K082003
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_K1631000
sequence annotation: BBa_B0034
sequence annotation: BBa_K082003
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1631001_sequence/1

BBa_K1631000
type: Component
persistent identity: component2455480
display id: component2455480
version: 1
top level: BBa_K1631001
definition: BBa_K1631000
access: public

BBa_K082003
type: Component
persistent identity: component2455485
display id: component2455485
version: 1
top level: BBa_K1631001
definition: BBa_K082003
access: public

BBa_B0034
type: Component
persistent identity: component2455482
display id: component2455482
version: 1
top level: BBa_K1631001
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component2455488
display id: component2455488
version: 1
top level: BBa_K1631001
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2455486
display id: component2455486
version: 1
top level: BBa_K1631001
definition: BBa_B0010
access: public

BBa_K1631000
type: SequenceAnnotation
persistent identity: annotation2455480
display id: annotation2455480
version: 1
top level: BBa_K1631001
location: https://synbiohub.org/public/igem/BBa_K1631001/annotation2455480/range2455480/1
component: BBa_K1631000

https://synbiohub.org/public/igem/BBa_K1631001/annotation2455480/range2455480/1
type: Range
persistent identity: range2455480
display id: range2455480
version: 1
top level: BBa_K1631001
direction: direction/reverse
start: 1
end: 348
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2455482
display id: annotation2455482
version: 1
top level: BBa_K1631001
location: https://synbiohub.org/public/igem/BBa_K1631001/annotation2455482/range2455482/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1631001/annotation2455482/range2455482/1
type: Range
persistent identity: range2455482
display id: range2455482
version: 1
top level: BBa_K1631001
direction: direction/reverse
start: 357
end: 368
orientation: inline

BBa_K082003
type: SequenceAnnotation
persistent identity: annotation2455485
display id: annotation2455485
version: 1
top level: BBa_K1631001
location: https://synbiohub.org/public/igem/BBa_K1631001/annotation2455485/range2455485/1
component: BBa_K082003

https://synbiohub.org/public/igem/BBa_K1631001/annotation2455485/range2455485/1
type: Range
persistent identity: range2455485
display id: range2455485
version: 1
top level: BBa_K1631001
direction: direction/reverse
start: 375
end: 1130
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2455486
display id: annotation2455486
version: 1
top level: BBa_K1631001
location: https://synbiohub.org/public/igem/BBa_K1631001/annotation2455486/range2455486/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1631001/annotation2455486/range2455486/1
type: Range
persistent identity: range2455486
display id: range2455486
version: 1
top level: BBa_K1631001
direction: direction/forward
start: 1139
end: 1218
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2455488
display id: annotation2455488
version: 1
top level: BBa_K1631001
location: https://synbiohub.org/public/igem/BBa_K1631001/annotation2455488/range2455488/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1631001/annotation2455488/range2455488/1
type: Range
persistent identity: range2455488
display id: range2455488
version: 1
top level: BBa_K1631001
direction: direction/forward
start: 1227
end: 1267
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K1631000
type: ComponentDefinition
persistent identity: BBa K1631000
display id: BBa_K1631000
version: 1
was generated by: iGEM to SBOL conversion
description: Translational unit of Barnase
created: 2015-09-11T11:00:00Z
modified: 2015-09-14T07:28:13Z
mutable provenance: BBa_I7161211
owned by: james
owned by: myers
mutable description: This part is improvement of BBa_I716211. We added a start codon and charactarized it. If you want to know more detail, visit our wiki. Barnase is a RNase from Bacillus amyloliquefaciens. Its cytotoxity can be neutralized by immunity protein called Barstar (BBa_). This coding sequence only contains cataritic domain (not include extracellular translocation signal peptide of Bacillus amyloliquefaciens).
discontinued: false
dominant: false
experience: experience/None
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: adding start codon
star: false
creator: Yuto Yamanaka
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: BBa_B0034
sequence annotation: ATG Start Codon
sequence annotation: Barnase catalitic domain ( I716211 )
sequence: https://synbiohub.org/public/igem/BBa_K1631000_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2455461
display id: annotation2455461
version: 1
top level: BBa_K1631000
location: https://synbiohub.org/public/igem/BBa_K1631000/annotation2455461/range2455461/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1631000/annotation2455461/range2455461/1
type: Range
persistent identity: range2455461
display id: range2455461
version: 1
top level: BBa_K1631000
direction: direction/forward
start: 1
end: 12
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2452453
display id: annotation2452453
version: 1
top level: BBa_K1631000
location: https://synbiohub.org/public/igem/BBa_K1631000/annotation2452453/range2452453/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1631000/annotation2452453/range2452453/1
type: Range
persistent identity: range2452453
display id: range2452453
version: 1
top level: BBa_K1631000
direction: direction/forward
start: 13
end: 15
orientation: inline

Barnase catalitic domain ( I716211 )
type: SequenceAnnotation
persistent identity: annotation2452447
display id: annotation2452447
version: 1
top level: BBa_K1631000
location: https://synbiohub.org/public/igem/BBa_K1631000/annotation2452447/range2452447/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1631000/annotation2452447/range2452447/1
type: Range
persistent identity: range2452447
display id: range2452447
version: 1
top level: BBa_K1631000
direction: direction/forward
start: 16
end: 348
orientation: inline

BBa_K082003
type: ComponentDefinition
persistent identity: BBa K082003
display id: BBa_K082003
version: 1
was generated by: iGEM to SBOL conversion
description: GFP(+LVA)
created: 2008-10-27T12:00:00Z
modified: 2016-01-25T04:43:13Z
mutable provenance: none
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: noen
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 4206
owner id: 1560
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: none
star: false
creator: Yanting Xue , He Shen
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_K082003_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation1992959
display id: annotation1992959
version: 1
top level: BBa_K082003
location: https://synbiohub.org/public/igem/BBa_K082003/annotation1992959/range1992959/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K082003/annotation1992959/range1992959/1
type: Range
persistent identity: range1992959
display id: range1992959
version: 1
top level: BBa_K082003
direction: direction/forward
start: 1
end: 720
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1993327
display id: annotation1993327
version: 1
top level: BBa_K082003
location: https://synbiohub.org/public/igem/BBa_K082003/annotation1993327/range1993327/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K082003/annotation1993327/range1993327/1
type: Range
persistent identity: range1993327
display id: range1993327
version: 1
top level: BBa_K082003
direction: direction/forward
start: 721
end: 753
orientation: inline

https://synbiohub.org/public/igem/BBa_K1631022_sequence/1
type: Sequence
persistent identity: BBa K1631022 sequence
display id: BBa_K1631022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaaatggcacaggttatcaacacgtttgacggggttgcggattatcttcagacatatcataagctacctgataattacattacaaaatcagaagcacaagccctcggctgggtggcatcaaaagggaaccttgcagacgtcgctccggggaaaagcatcggcggagacatcttctcaaacagggaaggcaaactcccgggcaaaagcggacgaacatggcgtgaagcggatattaactatacatcaggcttcagaaattcagaccggattctttactcaagcgactggctgatttacaaaacaacggaccattatcagacctttacaaaaatcagataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1631001_sequence/1
type: Sequence
persistent identity: BBa K1631001 sequence
display id: BBa_K1631001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaaatggcacaggttatcaacacgtttgacggggttgcggattatcttcagacatatcataagctacctgataattacattacaaaatcagaagcacaagccctcggctgggtggcatcaaaagggaaccttgcagacgtcgctccggggaaaagcatcggcggagacatcttctcaaacagggaaggcaaactcccgggcaaaagcggacgaacatggcgtgaagcggatattaactatacatcaggcttcagaaattcagaccggattctttactcaagcgactggctgatttacaaaacaacggaccattatcagacctttacaaaaatcagataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1631000_sequence/1
type: Sequence
persistent identity: BBa K1631000 sequence
display id: BBa_K1631000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaaatggcacaggttatcaacacgtttgacggggttgcggattatcttcagacatatcataagctacctgataattacattacaaaatcagaagcacaagccctcggctgggtggcatcaaaagggaaccttgcagacgtcgctccggggaaaagcatcggcggagacatcttctcaaacagggaaggcaaactcccgggcaaaagcggacgaacatggcgtgaagcggatattaactatacatcaggcttcagaaattcagaccggattctttactcaagcgactggctgatttacaaaacaacggaccattatcagacctttacaaaaatcagataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K082003_sequence/1
type: Sequence
persistent identity: BBa K082003 sequence
display id: BBa_K082003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z