##gff-version 3
##sequence-region BBa_K1631024 1 2290
BBa_K1631024	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_K1631024	.	engineered_region	44	2290	.	+	0	ID=BBa_K1631009;Name=BBa_K1631009
BBa_K1631024	.	engineered_region	208	1875	.	+	0	ID=BBa_K1631002;Name=BBa_K1631002;Parent=BBa_K1631009
BBa_K1631024	.	ribosome_entry_site	208	219	.	+	0	ID=annotation2452986;Name=BBa_B0034;Parent=BBa_K1631002
BBa_K1631024	.	CDS	220	1875	.	+	0	ID=annotation2452987;Name=Colicin-E3;Parent=BBa_K1631002
BBa_K1631024	.	terminator	2162	2290	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1631009
BBa_K1631024	.	terminator	2162	2241	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1631024	.	engineered_region	2162	2241	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1631024	.	stem_loop	2173	2216	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1631024	.	terminator	2250	2290	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1631024	.	engineered_region	2250	2290	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1631024	.	stop_codon	2283	2283	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1631024	.	stem_loop	2257	2276	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1631024	.	polyA_site	2277	2290	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1631024	.	engineered_region	44	199	.	+	0	ID=BBa_K1631003;Name=BBa_K1631003;Parent=BBa_K1631009
BBa_K1631024	.	ribosome_entry_site	44	55	.	+	0	ID=annotation2453045;Name=BBa_B0034;Parent=BBa_K1631003
BBa_K1631024	.	CDS	56	199	.	+	0	ID=annotation2453046;Name=Colicin Lysis Protein ;Parent=BBa_K1631003
BBa_K1631024	.	engineered_region	1884	2153	.	+	0	ID=BBa_K1631004;Name=BBa_K1631004;Parent=BBa_K1631009
BBa_K1631024	.	ribosome_entry_site	1884	1895	.	+	0	ID=annotation2453060;Name=BBa_B0034;Parent=BBa_K1631004
BBa_K1631024	.	CDS	1896	2153	.	+	0	ID=annotation2453061;Name=Colicin Immunity protein;Parent=BBa_K1631004
>BBa_K1631024
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaaatgaaaaaaatcaccggtatcatc
ctactactgctggcagttatcatcctgtctgcatgccaggcgaactacatccgtgacgttcagggtggtacagtttctc
catcttctaccgcggaagttaccggtctagcaactcagtaatactagagaaagaggagaaaatgtctggcggtgacggt
cgtggtcacaatactggtgctcattctacatctggcaacatcaacggcggtcctactggtcttggtgttggtggcggtg
catctgacggttctggctggtcttccgagaacaatccatggggtggcggttctggttctggtattcactggggcggtgg
ctctggtcatggtaacggtggcggtaatggtaactccggtggcggttctggtaccggtggtaacctgtctgcggttgca
gctccagttgcattcggtttcccagcactatctactcctggtgcaggtggtctagcagtttctatctcagcaggtgcac
tgtcagcagcaatcgcggatatcatggcagcgctgaaaggtccattcaaattcggtctgtggggtgttgcgctgtacgg
tgttctgccgtctcagatcgcgaaagacgacccgaacatgatgtctaaaatcgttacatctctgccggcggacgacatc
accgaatctccggtttcttctctgccgctggacaaggctaccgttaatgtcaacgtacgtgtagttgatgacgttaaag
acgaacgtcagaacatatctgtagtttctggtgttccgatgtctgttccagtcgttgatgctaagcctactgagcgtcc
aggcgtattcactgcgtctatacctggcgctccagtactgaacatctccgtcaataactctactccagcggttcagact
ctatctccaggcgttactaataacaccgacaaagacgtacgaccagcaggcttcactcaaggaggtaatacacgtgacg
cggttatccgattcccgaaagactccggtcataatgcggtctacgtatctgtatctgacgtactgtctccggatcaggt
caagcagcgtcaggacgaggaaaaccgccgtcaacaggagtgggacgcaactcatccagttgaagcagctgagcgtaac
tacgagcgtgcacgtgctgagctgaatcaggcgaacgaggacgttgcacgtaatcaagagcgtcaggcgaaagcggtac
aggtctacaattctcgtaagtccgaactggacgcagctaacaagacacttgcagacgcaatcgcggaaatcaaacagtt
caaccgtttcgcacatgaccctatggcaggtggtcaccgtatgtggcagatggcaggtctgaaagcacagcgtgcacag
accgacgttaacaacaaacaagcagcgttcgacgcggcggcgaaagaaaaatctgacgcagacgcagcactgtcttctg
cgatggaatctcgtaaaaaaaaagaagacaaaaaacgttctgcggaaaacaacctgaacgacgaaaaaaacaaaccgcg
taaaggtttcaaagactacggtcacgactaccatccagctccgaagactgagaacatcaaaggtctaggcgacctgaaa
ccaggtatcccgaaaaccccgaaacagaacggtggtggtaaacgtaaacgttggaccggtgacaaaggtcgtaagatct
acgagtgggattctcagcacggtgagctagaaggctatcgtgcatctgacggtcagcacctgggttctttcgacccaaa
aaccggtaaccagctgaaaggtccagacccgaaacgtaacatcaaaaaatacctgtaatactagagaaagaggagaaaa
tgggtctgaaactggacctgacctggttcgacaaatctaccgaagacttcaaaggtgaagaatactctaaagacttcgg
tgacgacggttctgttatggaatctctgggtgttccgttcaaagacaacgttaacaacggttgcttcgacgttatcgcg
gaatgggttccactactacagccgtacttcaaccaccagatcgacatctctgacaacgaatacttcgtttctttcgact
accgtgacggtgactggtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata