LOCUS       BBa_K1631025            2290 bp    DNA     linear       25-Nov-2024
DEFINITION  Colicin-E3 cassette (Medium)
ACCESSION   BBa_K1631025
VERSION     BBa_K1631025.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     RBS             44..55
                     /label=BBa_B0034
     CDS             56..199
                     /label=Colicin Lysis Protein 
     misc_feature    44..199
                     /label=BBa_K1631003
     RBS             208..219
                     /label=BBa_B0034
     CDS             220..1875
                     /label=Colicin-E3
     misc_feature    208..1875
                     /label=BBa_K1631002
     RBS             1884..1895
                     /label=BBa_B0034
     CDS             1896..2153
                     /label=Colicin Immunity protein
     misc_feature    1884..2153
                     /label=BBa_K1631004
     stem_loop       2173..2216
                     /label=stem_loop
     misc_feature    2162..2241
                     /label=BBa_B0010
     terminator      2162..2241
                     /label=BBa_B0010
     stem_loop       2257..2276
                     /label=T7 TE
     misc_feature    2283..2283
                     /label=stop
     polyA_site      2277..2290
                     /label=polya
     misc_feature    2250..2290
                     /label=BBa_B0012
     terminator      2250..2290
                     /label=BBa_B0012
     terminator      2162..2290
                     /label=BBa_B0015
     misc_feature    44..2290
                     /label=BBa_K1631009
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaaatgaa
       61 aaaaatcacc ggtatcatcc tactactgct ggcagttatc atcctgtctg catgccaggc
      121 gaactacatc cgtgacgttc agggtggtac agtttctcca tcttctaccg cggaagttac
      181 cggtctagca actcagtaat actagagaaa gaggagaaaa tgtctggcgg tgacggtcgt
      241 ggtcacaata ctggtgctca ttctacatct ggcaacatca acggcggtcc tactggtctt
      301 ggtgttggtg gcggtgcatc tgacggttct ggctggtctt ccgagaacaa tccatggggt
      361 ggcggttctg gttctggtat tcactggggc ggtggctctg gtcatggtaa cggtggcggt
      421 aatggtaact ccggtggcgg ttctggtacc ggtggtaacc tgtctgcggt tgcagctcca
      481 gttgcattcg gtttcccagc actatctact cctggtgcag gtggtctagc agtttctatc
      541 tcagcaggtg cactgtcagc agcaatcgcg gatatcatgg cagcgctgaa aggtccattc
      601 aaattcggtc tgtggggtgt tgcgctgtac ggtgttctgc cgtctcagat cgcgaaagac
      661 gacccgaaca tgatgtctaa aatcgttaca tctctgccgg cggacgacat caccgaatct
      721 ccggtttctt ctctgccgct ggacaaggct accgttaatg tcaacgtacg tgtagttgat
      781 gacgttaaag acgaacgtca gaacatatct gtagtttctg gtgttccgat gtctgttcca
      841 gtcgttgatg ctaagcctac tgagcgtcca ggcgtattca ctgcgtctat acctggcgct
      901 ccagtactga acatctccgt caataactct actccagcgg ttcagactct atctccaggc
      961 gttactaata acaccgacaa agacgtacga ccagcaggct tcactcaagg aggtaataca
     1021 cgtgacgcgg ttatccgatt cccgaaagac tccggtcata atgcggtcta cgtatctgta
     1081 tctgacgtac tgtctccgga tcaggtcaag cagcgtcagg acgaggaaaa ccgccgtcaa
     1141 caggagtggg acgcaactca tccagttgaa gcagctgagc gtaactacga gcgtgcacgt
     1201 gctgagctga atcaggcgaa cgaggacgtt gcacgtaatc aagagcgtca ggcgaaagcg
     1261 gtacaggtct acaattctcg taagtccgaa ctggacgcag ctaacaagac acttgcagac
     1321 gcaatcgcgg aaatcaaaca gttcaaccgt ttcgcacatg accctatggc aggtggtcac
     1381 cgtatgtggc agatggcagg tctgaaagca cagcgtgcac agaccgacgt taacaacaaa
     1441 caagcagcgt tcgacgcggc ggcgaaagaa aaatctgacg cagacgcagc actgtcttct
     1501 gcgatggaat ctcgtaaaaa aaaagaagac aaaaaacgtt ctgcggaaaa caacctgaac
     1561 gacgaaaaaa acaaaccgcg taaaggtttc aaagactacg gtcacgacta ccatccagct
     1621 ccgaagactg agaacatcaa aggtctaggc gacctgaaac caggtatccc gaaaaccccg
     1681 aaacagaacg gtggtggtaa acgtaaacgt tggaccggtg acaaaggtcg taagatctac
     1741 gagtgggatt ctcagcacgg tgagctagaa ggctatcgtg catctgacgg tcagcacctg
     1801 ggttctttcg acccaaaaac cggtaaccag ctgaaaggtc cagacccgaa acgtaacatc
     1861 aaaaaatacc tgtaatacta gagaaagagg agaaaatggg tctgaaactg gacctgacct
     1921 ggttcgacaa atctaccgaa gacttcaaag gtgaagaata ctctaaagac ttcggtgacg
     1981 acggttctgt tatggaatct ctgggtgttc cgttcaaaga caacgttaac aacggttgct
     2041 tcgacgttat cgcggaatgg gttccactac tacagccgta cttcaaccac cagatcgaca
     2101 tctctgacaa cgaatacttc gtttctttcg actaccgtga cggtgactgg taatactaga
     2161 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     2221 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2281 tgcgtttata
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