BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K1631009 1 BBa_K1631009 rbs - Colicin Lysis prrotein - rbs -ColicinE3 - rbs - Colicin Immunity protein - d.term 2015-09-13T11:00:00Z 2015-09-14T07:20:09Z Assembly Colicin cassete without promoter. false false _2048_ 23567 23567 9 false none false Yuto Yamanaka component2455460 1 BBa_B0015 component2455447 1 BBa_K1631003 component2455450 1 BBa_K1631002 component2455453 1 BBa_K1631004 annotation2455460 1 BBa_B0015 range2455460 1 2119 2247 annotation2455450 1 BBa_K1631002 range2455450 1 165 1832 annotation2455447 1 BBa_K1631003 range2455447 1 1 156 annotation2455453 1 BBa_K1631004 range2455453 1 1841 2110 BBa_K1631004 1 BBa_K1631004 Tlanslational unit of Immunity protein for Colicin-E3 2015-09-12T11:00:00Z 2015-09-13T12:52:31Z pColE3-CA38 Immunity protein for Colicin-E3 binds in between the Translocation domain and Ctalitic domain. Curiously, immunity protein does not block the active site.[1] It is considered that immunity protein blocks access of this enzyme to the ribosome. Colicins are a cytotoxins which are released to environment and kill other related strains. false false _2048_ 23567 23567 9 false none false Yuto Yamanaka annotation2453061 1 Colicin Immunity protein range2453061 1 13 270 annotation2453060 1 BBa_B0034 range2453060 1 1 12 BBa_K1631002 1 BBa_K1631002 Translational unit of Colicin-E3 2015-09-11T11:00:00Z 2015-09-14T03:43:50Z pColE3-CA38 Colicin-E3 cleave 16SrRNA at a specific site. This activity is inhibited by immunity protein for Colicin-E3(BBa_). When lysis protein for Colicin-E3(BBa_) is co-expressed, Colicin-E3 is emited to environment and kill other strain. Colicin is a type of bacteriocin produced by Escherichia coli. false false _2048_ 23567 23567 9 false none false Yuto Yamanaka annotation2452987 1 Colicin-E3 range2452987 1 13 1668 annotation2452986 1 BBa_B0034 range2452986 1 1 12 BBa_J23110 1 BBa_J23110 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1631003 1 BBa_K1631003 Tlanslational unit of Colicin Lysis Protein (for colicin-E3) 2015-09-12T11:00:00Z 2015-09-18T06:28:24Z pColE3-CA38 Colicin lysis protein allows colicins to be released. The mechanism how the colicin lysis protein allows colicin release has not been fully elucidated, but it is sure that this protein raise membrane permiability and cause quasilysis.[1] Colicins are a cytotoxins which are released to environment and kill other related strains. false false _2048_ 23567 23567 9 false none false Yuto Yamanaka annotation2453045 1 BBa_B0034 range2453045 1 1 12 annotation2453046 1 Colicin Lysis Protein range2453046 1 13 156 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K1631025 1 BBa_K1631025 Colicin-E3 cassette (Medium) 2015-09-13T11:00:00Z 2015-09-18T09:38:28Z Assembly Colicin-E3, its immunity protein, and colisin lysis protein is constitutively expressed.(under BBa_J23110) When this device works, Colicin-E3 is released to media, and kill E. coli strains which does not have immunity protein. false false _2048_ 23567 23567 9 false none false Yuto Yamanaka component2455808 1 BBa_J23110 component2455825 1 BBa_K1631009 annotation2455825 1 BBa_K1631009 range2455825 1 44 2290 annotation2455808 1 BBa_J23110 range2455808 1 1 35 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1631003_sequence 1 aaagaggagaaaatgaaaaaaatcaccggtatcatcctactactgctggcagttatcatcctgtctgcatgccaggcgaactacatccgtgacgttcagggtggtacagtttctccatcttctaccgcggaagttaccggtctagcaactcagtaa BBa_K1631002_sequence 1 aaagaggagaaaatgtctggcggtgacggtcgtggtcacaatactggtgctcattctacatctggcaacatcaacggcggtcctactggtcttggtgttggtggcggtgcatctgacggttctggctggtcttccgagaacaatccatggggtggcggttctggttctggtattcactggggcggtggctctggtcatggtaacggtggcggtaatggtaactccggtggcggttctggtaccggtggtaacctgtctgcggttgcagctccagttgcattcggtttcccagcactatctactcctggtgcaggtggtctagcagtttctatctcagcaggtgcactgtcagcagcaatcgcggatatcatggcagcgctgaaaggtccattcaaattcggtctgtggggtgttgcgctgtacggtgttctgccgtctcagatcgcgaaagacgacccgaacatgatgtctaaaatcgttacatctctgccggcggacgacatcaccgaatctccggtttcttctctgccgctggacaaggctaccgttaatgtcaacgtacgtgtagttgatgacgttaaagacgaacgtcagaacatatctgtagtttctggtgttccgatgtctgttccagtcgttgatgctaagcctactgagcgtccaggcgtattcactgcgtctatacctggcgctccagtactgaacatctccgtcaataactctactccagcggttcagactctatctccaggcgttactaataacaccgacaaagacgtacgaccagcaggcttcactcaaggaggtaatacacgtgacgcggttatccgattcccgaaagactccggtcataatgcggtctacgtatctgtatctgacgtactgtctccggatcaggtcaagcagcgtcaggacgaggaaaaccgccgtcaacaggagtgggacgcaactcatccagttgaagcagctgagcgtaactacgagcgtgcacgtgctgagctgaatcaggcgaacgaggacgttgcacgtaatcaagagcgtcaggcgaaagcggtacaggtctacaattctcgtaagtccgaactggacgcagctaacaagacacttgcagacgcaatcgcggaaatcaaacagttcaaccgtttcgcacatgaccctatggcaggtggtcaccgtatgtggcagatggcaggtctgaaagcacagcgtgcacagaccgacgttaacaacaaacaagcagcgttcgacgcggcggcgaaagaaaaatctgacgcagacgcagcactgtcttctgcgatggaatctcgtaaaaaaaaagaagacaaaaaacgttctgcggaaaacaacctgaacgacgaaaaaaacaaaccgcgtaaaggtttcaaagactacggtcacgactaccatccagctccgaagactgagaacatcaaaggtctaggcgacctgaaaccaggtatcccgaaaaccccgaaacagaacggtggtggtaaacgtaaacgttggaccggtgacaaaggtcgtaagatctacgagtgggattctcagcacggtgagctagaaggctatcgtgcatctgacggtcagcacctgggttctttcgacccaaaaaccggtaaccagctgaaaggtccagacccgaaacgtaacatcaaaaaatacctgtaa BBa_K1631004_sequence 1 aaagaggagaaaatgggtctgaaactggacctgacctggttcgacaaatctaccgaagacttcaaaggtgaagaatactctaaagacttcggtgacgacggttctgttatggaatctctgggtgttccgttcaaagacaacgttaacaacggttgcttcgacgttatcgcggaatgggttccactactacagccgtacttcaaccaccagatcgacatctctgacaacgaatacttcgtttctttcgactaccgtgacggtgactggtaa BBa_K1631009_sequence 1 aaagaggagaaaatgaaaaaaatcaccggtatcatcctactactgctggcagttatcatcctgtctgcatgccaggcgaactacatccgtgacgttcagggtggtacagtttctccatcttctaccgcggaagttaccggtctagcaactcagtaatactagagaaagaggagaaaatgtctggcggtgacggtcgtggtcacaatactggtgctcattctacatctggcaacatcaacggcggtcctactggtcttggtgttggtggcggtgcatctgacggttctggctggtcttccgagaacaatccatggggtggcggttctggttctggtattcactggggcggtggctctggtcatggtaacggtggcggtaatggtaactccggtggcggttctggtaccggtggtaacctgtctgcggttgcagctccagttgcattcggtttcccagcactatctactcctggtgcaggtggtctagcagtttctatctcagcaggtgcactgtcagcagcaatcgcggatatcatggcagcgctgaaaggtccattcaaattcggtctgtggggtgttgcgctgtacggtgttctgccgtctcagatcgcgaaagacgacccgaacatgatgtctaaaatcgttacatctctgccggcggacgacatcaccgaatctccggtttcttctctgccgctggacaaggctaccgttaatgtcaacgtacgtgtagttgatgacgttaaagacgaacgtcagaacatatctgtagtttctggtgttccgatgtctgttccagtcgttgatgctaagcctactgagcgtccaggcgtattcactgcgtctatacctggcgctccagtactgaacatctccgtcaataactctactccagcggttcagactctatctccaggcgttactaataacaccgacaaagacgtacgaccagcaggcttcactcaaggaggtaatacacgtgacgcggttatccgattcccgaaagactccggtcataatgcggtctacgtatctgtatctgacgtactgtctccggatcaggtcaagcagcgtcaggacgaggaaaaccgccgtcaacaggagtgggacgcaactcatccagttgaagcagctgagcgtaactacgagcgtgcacgtgctgagctgaatcaggcgaacgaggacgttgcacgtaatcaagagcgtcaggcgaaagcggtacaggtctacaattctcgtaagtccgaactggacgcagctaacaagacacttgcagacgcaatcgcggaaatcaaacagttcaaccgtttcgcacatgaccctatggcaggtggtcaccgtatgtggcagatggcaggtctgaaagcacagcgtgcacagaccgacgttaacaacaaacaagcagcgttcgacgcggcggcgaaagaaaaatctgacgcagacgcagcactgtcttctgcgatggaatctcgtaaaaaaaaagaagacaaaaaacgttctgcggaaaacaacctgaacgacgaaaaaaacaaaccgcgtaaaggtttcaaagactacggtcacgactaccatccagctccgaagactgagaacatcaaaggtctaggcgacctgaaaccaggtatcccgaaaaccccgaaacagaacggtggtggtaaacgtaaacgttggaccggtgacaaaggtcgtaagatctacgagtgggattctcagcacggtgagctagaaggctatcgtgcatctgacggtcagcacctgggttctttcgacccaaaaaccggtaaccagctgaaaggtccagacccgaaacgtaacatcaaaaaatacctgtaatactagagaaagaggagaaaatgggtctgaaactggacctgacctggttcgacaaatctaccgaagacttcaaaggtgaagaatactctaaagacttcggtgacgacggttctgttatggaatctctgggtgttccgttcaaagacaacgttaacaacggttgcttcgacgttatcgcggaatgggttccactactacagccgtacttcaaccaccagatcgacatctctgacaacgaatacttcgtttctttcgactaccgtgacggtgactggtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23110_sequence 1 tttacggctagctcagtcctaggtacaatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1631025_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaaatgaaaaaaatcaccggtatcatcctactactgctggcagttatcatcctgtctgcatgccaggcgaactacatccgtgacgttcagggtggtacagtttctccatcttctaccgcggaagttaccggtctagcaactcagtaatactagagaaagaggagaaaatgtctggcggtgacggtcgtggtcacaatactggtgctcattctacatctggcaacatcaacggcggtcctactggtcttggtgttggtggcggtgcatctgacggttctggctggtcttccgagaacaatccatggggtggcggttctggttctggtattcactggggcggtggctctggtcatggtaacggtggcggtaatggtaactccggtggcggttctggtaccggtggtaacctgtctgcggttgcagctccagttgcattcggtttcccagcactatctactcctggtgcaggtggtctagcagtttctatctcagcaggtgcactgtcagcagcaatcgcggatatcatggcagcgctgaaaggtccattcaaattcggtctgtggggtgttgcgctgtacggtgttctgccgtctcagatcgcgaaagacgacccgaacatgatgtctaaaatcgttacatctctgccggcggacgacatcaccgaatctccggtttcttctctgccgctggacaaggctaccgttaatgtcaacgtacgtgtagttgatgacgttaaagacgaacgtcagaacatatctgtagtttctggtgttccgatgtctgttccagtcgttgatgctaagcctactgagcgtccaggcgtattcactgcgtctatacctggcgctccagtactgaacatctccgtcaataactctactccagcggttcagactctatctccaggcgttactaataacaccgacaaagacgtacgaccagcaggcttcactcaaggaggtaatacacgtgacgcggttatccgattcccgaaagactccggtcataatgcggtctacgtatctgtatctgacgtactgtctccggatcaggtcaagcagcgtcaggacgaggaaaaccgccgtcaacaggagtgggacgcaactcatccagttgaagcagctgagcgtaactacgagcgtgcacgtgctgagctgaatcaggcgaacgaggacgttgcacgtaatcaagagcgtcaggcgaaagcggtacaggtctacaattctcgtaagtccgaactggacgcagctaacaagacacttgcagacgcaatcgcggaaatcaaacagttcaaccgtttcgcacatgaccctatggcaggtggtcaccgtatgtggcagatggcaggtctgaaagcacagcgtgcacagaccgacgttaacaacaaacaagcagcgttcgacgcggcggcgaaagaaaaatctgacgcagacgcagcactgtcttctgcgatggaatctcgtaaaaaaaaagaagacaaaaaacgttctgcggaaaacaacctgaacgacgaaaaaaacaaaccgcgtaaaggtttcaaagactacggtcacgactaccatccagctccgaagactgagaacatcaaaggtctaggcgacctgaaaccaggtatcccgaaaaccccgaaacagaacggtggtggtaaacgtaaacgttggaccggtgacaaaggtcgtaagatctacgagtgggattctcagcacggtgagctagaaggctatcgtgcatctgacggtcagcacctgggttctttcgacccaaaaaccggtaaccagctgaaaggtccagacccgaaacgtaacatcaaaaaatacctgtaatactagagaaagaggagaaaatgggtctgaaactggacctgacctggttcgacaaatctaccgaagacttcaaaggtgaagaatactctaaagacttcggtgacgacggttctgttatggaatctctgggtgttccgttcaaagacaacgttaacaacggttgcttcgacgttatcgcggaatgggttccactactacagccgtacttcaaccaccagatcgacatctctgacaacgaatacttcgtttctttcgactaccgtgacggtgactggtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z