LOCUS       BBa_K1632023            1710 bp    DNA     linear       23-Dec-2024
DEFINITION  J23100_rbs_rhlR_TT_Plux_rbs_CmRssrA
ACCESSION   BBa_K1632023
VERSION     BBa_K1632023.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..790
                     /label=BBa_C0171
     stem_loop       810..853
                     /label=stem_loop
     misc_feature    799..878
                     /label=BBa_B0010
     terminator      799..878
                     /label=BBa_B0010
     stem_loop       894..913
                     /label=T7 TE
     misc_feature    920..920
                     /label=stop
     polyA_site      914..927
                     /label=polya
     misc_feature    887..927
                     /label=BBa_B0012
     terminator      887..927
                     /label=BBa_B0012
     misc_feature    44..927
                     /label=BBa_I1466
     misc_feature    936..955
                     /label=LuxR/HSL
     promoter        955..960
                     /label=-35
     promoter        977..982
                     /label=-10
     misc_feature    988..988
                     /label=start
     misc_feature    936..990
                     /label=BBa_R0062
     promoter        936..990
                     /label=BBa_R0062
     RBS             1004..1007
                     /label=RBS
     misc_feature    1015..1675
                     /label=CmR
     misc_feature    999..1677
                     /label=BBa_K395160
     misc_feature    1686..1718
                     /label=AANDENYADAS  SsrA degradation tag
     misc_feature    1686..1718
                     /label=BBa_M0052
     misc_feature    999..1710
                     /label=BBa_K1632020
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc
      121 gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt
      181 cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga agaccgaggt
      241 ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact acggggccgt
      301 ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct
      361 gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct gtgtcggcgc
      421 gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca
      481 gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt gcatgatcga
      541 gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca acccggtctg
      601 cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga gttccgggga
      661 aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca agaacatcca
      721 gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg cgctgggtct
      781 catctaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      841 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      901 cttcgggtgg gcctttctgc gtttatatac tagagacctg taggatcgta caggtttacg
      961 caagaaaatg gtttgttata gtcgaataaa tactagagag ctaaggaagc taaaatggag
     1021 aaaaaaatca cgggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     1081 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     1141 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     1201 cttgcccgcc tgatgaacgc tcacccggag tttcgtatgg ccatgaaaga cggtgagctg
     1261 gtgatctggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     1321 tcgtccctct ggagtgaata ccacgacgat ttccggcagt ttctccacat atattcgcaa
     1381 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     1441 ttttttgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     1501 atggacaact tcttcgcccc cgttttcacg atgggcaaat attatacgca aggcgacaag
     1561 gtgctgatgc cgctggcgat ccaggttcat catgccgttt gtgatggctt ccatgtcggc
     1621 cgcatgctta atgaattaca acagtactgt gatgagtggc agggcggggc ggctgctaac
     1681 gacgaaaact acgctctggc tgcttaataa
//