BBa_K1632023
type: ComponentDefinition
persistent identity: BBa K1632023
display id: BBa_K1632023
version: 1
was generated by: iGEM to SBOL conversion
description: J23100_rbs_rhlR_TT_Plux_rbs_CmRssrA
created: 2015-08-29T11:00:00Z
modified: 2015-09-17T06:15:54Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 24760
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Jun kawamura
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I1466
component: BBa_J23100
component: BBa_K1632020
component: BBa_R0062
sequence annotation: BBa_J23100
sequence annotation: BBa_I1466
sequence annotation: BBa_R0062
sequence annotation: BBa_K1632020
sequence: https://synbiohub.org/public/igem/BBa_K1632023_sequence/1

BBa_I1466
type: Component
persistent identity: component2441382
display id: component2441382
version: 1
top level: BBa_K1632023
definition: BBa_I1466
access: public

BBa_J23100
type: Component
persistent identity: component2441372
display id: component2441372
version: 1
top level: BBa_K1632023
definition: BBa_J23100
access: public

BBa_K1632020
type: Component
persistent identity: component2441393
display id: component2441393
version: 1
top level: BBa_K1632023
definition: BBa_K1632020
access: public

BBa_R0062
type: Component
persistent identity: component2441384
display id: component2441384
version: 1
top level: BBa_K1632023
definition: lux pR
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2441372
display id: annotation2441372
version: 1
top level: BBa_K1632023
location: https://synbiohub.org/public/igem/BBa_K1632023/annotation2441372/range2441372/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1632023/annotation2441372/range2441372/1
type: Range
persistent identity: range2441372
display id: range2441372
version: 1
top level: BBa_K1632023
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_I1466
type: SequenceAnnotation
persistent identity: annotation2441382
display id: annotation2441382
version: 1
top level: BBa_K1632023
location: https://synbiohub.org/public/igem/BBa_K1632023/annotation2441382/range2441382/1
component: BBa_I1466

https://synbiohub.org/public/igem/BBa_K1632023/annotation2441382/range2441382/1
type: Range
persistent identity: range2441382
display id: range2441382
version: 1
top level: BBa_K1632023
direction: direction/reverse
start: 44
end: 927
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2441384
display id: annotation2441384
version: 1
top level: BBa_K1632023
location: https://synbiohub.org/public/igem/BBa_K1632023/annotation2441384/range2441384/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1632023/annotation2441384/range2441384/1
type: Range
persistent identity: range2441384
display id: range2441384
version: 1
top level: BBa_K1632023
direction: direction/forward
start: 936
end: 990
orientation: inline

BBa_K1632020
type: SequenceAnnotation
persistent identity: annotation2441393
display id: annotation2441393
version: 1
top level: BBa_K1632023
location: https://synbiohub.org/public/igem/BBa_K1632023/annotation2441393/range2441393/1
component: BBa_K1632020

https://synbiohub.org/public/igem/BBa_K1632023/annotation2441393/range2441393/1
type: Range
persistent identity: range2441393
display id: range2441393
version: 1
top level: BBa_K1632023
direction: direction/reverse
start: 999
end: 1710
orientation: inline

BBa_K1632020
type: ComponentDefinition
persistent identity: BBa K1632020
display id: BBa_K1632020
version: 1
was generated by: iGEM to SBOL conversion
description: rbs_CmR(ssrA degradation tag)
created: 2015-08-29T11:00:00Z
modified: 2015-09-18T09:48:27Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 24760
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: a
star: false
creator: Jun kawamura
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: BBa_K395160
sequence annotation: RBS
sequence annotation: CmR
sequence annotation: BBa_M0052
sequence annotation: AANDENYADAS SsrA degradation tag
sequence: https://synbiohub.org/public/igem/BBa_K1632020_sequence/1

BBa_K395160
type: SequenceAnnotation
persistent identity: annotation2440306
display id: annotation2440306
version: 1
top level: BBa_K1632020
location: https://synbiohub.org/public/igem/BBa_K1632020/annotation2440306/range2440306/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1632020/annotation2440306/range2440306/1
type: Range
persistent identity: range2440306
display id: range2440306
version: 1
top level: BBa_K1632020
direction: direction/reverse
start: 1
end: 679
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2440304
display id: annotation2440304
version: 1
top level: BBa_K1632020
location: https://synbiohub.org/public/igem/BBa_K1632020/annotation2440304/range2440304/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1632020/annotation2440304/range2440304/1
type: Range
persistent identity: range2440304
display id: range2440304
version: 1
top level: BBa_K1632020
direction: direction/forward
start: 6
end: 9
orientation: inline

CmR
type: SequenceAnnotation
persistent identity: annotation2440305
display id: annotation2440305
version: 1
top level: BBa_K1632020
location: https://synbiohub.org/public/igem/BBa_K1632020/annotation2440305/range2440305/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1632020/annotation2440305/range2440305/1
type: Range
persistent identity: range2440305
display id: range2440305
version: 1
top level: BBa_K1632020
direction: direction/forward
start: 17
end: 677
orientation: inline

BBa_M0052
type: SequenceAnnotation
persistent identity: annotation2440308
display id: annotation2440308
version: 1
top level: BBa_K1632020
location: https://synbiohub.org/public/igem/BBa_K1632020/annotation2440308/range2440308/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1632020/annotation2440308/range2440308/1
type: Range
persistent identity: range2440308
display id: range2440308
version: 1
top level: BBa_K1632020
direction: direction/reverse
start: 688
end: 720
orientation: inline

AANDENYADAS SsrA degradation tag
type: SequenceAnnotation
persistent identity: annotation2440307
display id: annotation2440307
version: 1
top level: BBa_K1632020
location: https://synbiohub.org/public/igem/BBa_K1632020/annotation2440307/range2440307/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1632020/annotation2440307/range2440307/1
type: Range
persistent identity: range2440307
display id: range2440307
version: 1
top level: BBa_K1632020
direction: direction/forward
start: 688
end: 720
orientation: inline

BBa_I1466
type: ComponentDefinition
persistent identity: BBa I1466
display id: BBa_I1466
version: 1
was generated by: iGEM to SBOL conversion
description: RhlR protein generator (LVA-)
created: 2004-08-09T11:00:00Z
modified: 2015-08-31T04:07:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Elowitz RBS. RhlR CDS (no LVA tag) and terminator. Same as I0466 but no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Barry Canton
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_C0171
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0171
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I1466_sequence/1

BBa_C0171
type: Component
persistent identity: component1040125
display id: component1040125
version: 1
top level: BBa_I1466
definition: rhIR
access: public

BBa_B0034
type: Component
persistent identity: component1040123
display id: component1040123
version: 1
top level: BBa_I1466
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1040140
display id: component1040140
version: 1
top level: BBa_I1466
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1040130
display id: component1040130
version: 1
top level: BBa_I1466
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1040123
display id: annotation1040123
version: 1
top level: BBa_I1466
location: https://synbiohub.org/public/igem/BBa_I1466/annotation1040123/range1040123/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I1466/annotation1040123/range1040123/1
type: Range
persistent identity: range1040123
display id: range1040123
version: 1
top level: BBa_I1466
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0171
type: SequenceAnnotation
persistent identity: annotation1040125
display id: annotation1040125
version: 1
top level: BBa_I1466
location: https://synbiohub.org/public/igem/BBa_I1466/annotation1040125/range1040125/1
component: BBa_C0171

https://synbiohub.org/public/igem/BBa_I1466/annotation1040125/range1040125/1
type: Range
persistent identity: range1040125
display id: range1040125
version: 1
top level: BBa_I1466
direction: direction/forward
start: 19
end: 747
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1040130
display id: annotation1040130
version: 1
top level: BBa_I1466
location: https://synbiohub.org/public/igem/BBa_I1466/annotation1040130/range1040130/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I1466/annotation1040130/range1040130/1
type: Range
persistent identity: range1040130
display id: range1040130
version: 1
top level: BBa_I1466
direction: direction/forward
start: 756
end: 835
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1040140
display id: annotation1040140
version: 1
top level: BBa_I1466
location: https://synbiohub.org/public/igem/BBa_I1466/annotation1040140/range1040140/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I1466/annotation1040140/range1040140/1
type: Range
persistent identity: range1040140
display id: range1040140
version: 1
top level: BBa_I1466
direction: direction/forward
start: 844
end: 884
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

rhIR
type: ComponentDefinition
persistent identity: BBa C0171
display id: BBa_C0171
version: 1
was generated by: iGEM to SBOL conversion
description: rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same as C0071 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_C0171_sequence/1

https://synbiohub.org/public/igem/BBa_K1632023_sequence/1
type: Sequence
persistent identity: BBa K1632023 sequence
display id: BBa_K1632023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagagctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcggctgctaacgacgaaaactacgctctggctgcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1632020_sequence/1
type: Sequence
persistent identity: BBa K1632020 sequence
display id: BBa_K1632020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcggctgctaacgacgaaaactacgctctggctgcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I1466_sequence/1
type: Sequence
persistent identity: BBa I1466 sequence
display id: BBa_I1466_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0171_sequence/1
type: Sequence
persistent identity: BBa C0171 sequence
display id: BBa_C0171_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z