LOCUS BBa_K1632031 1871 bp DNA linear 08-May-2024 DEFINITION fim switch[default OFF](Tokyo_Tech/J23119)_rbs_gfp_rbs_rhlI ACCESSION BBa_K1632031 VERSION BBa_K1632031.1 FEATURES Location/Qualifiers misc_feature 84..91 /label=Asc1 misc_feature 92..97 /label=Bgl2 misc_feature 78..83 /label=Mlu1 promoter 98..132 /label=J23119 promoter misc_feature 133..138 /label=BamH1 misc_feature 139..146 /label=Asc1 misc_feature 147..152 /label=Sal1 promoter 1..432 /label=BBa_K1632001 misc_feature 445..448 /label=conserved RBS 441..452 /label=BBa_B0034 CDS 459..1178 /label=GFP protein CDS 459..1178 /label=BBa_E0040 misc_feature 1191..1194 /label=conserved RBS 1187..1198 /label=BBa_B0034 CDS 1205..1807 /label=rhlL misc_feature 1808..1840 /label=LVA misc_feature 1847..1871 /label=Help:Barcodes CDS 1205..1846 /label=BBa_C0070 misc_feature 1187..1871 /label=BBa_I9026 ORIGIN 1 tttgatttaa cttattgata ataaagttaa aaaaacaaat aaatacaaga caattggggc 61 caaactgtcc atatcatacg cgtggcgcgc cagatctgct agcattatac ctaggactga 121 gctagctgtc aaggatccgg cgcgccgtcg acgcataaaa atattaaaaa acgacaaaaa 181 gcatctaact gtttgatatg taaattattt ctattgtaaa ttaatttcac atcacctccg 241 ctatatgtaa agctaacgtt tctgtggctc gacgcatctt cctcattctt ctctccaaaa 301 accacctcat gcaatataaa catctataaa taaagataac aatagaatat taagccaaca 361 aataaactga aaaagtttgt ccgcgatgct ttcctctatg agtcaaaatg gccccaaatg 421 tttcatcttt tgtactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt 481 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt 541 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt 601 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg 661 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca 721 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga 781 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta 841 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac 901 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta 961 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa 1021 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt 1081 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg 1141 ggattacaca tggcatggat gaactataca aataataata ctagagaaag aggagaaata 1201 ctagatgatc gaactgctgt ccgaatccct ggaaggtctg tccgctgcta tgatcgctga 1261 actgggtcgt taccgtcacc aggttttcat cgaaaaactg ggttgggacg ttgtttccac 1321 ctcccgtgtt cgtgaccagg agttcgacca gttcgaccac ccgcagaccc gttacatcgt 1381 tgctatgtcc cgtcagggta tctgcggttg cgctcgtctg ctgccgacca ccgacgctta 1441 cctgctgaaa gacgttttcg cttacctgtg ctccgaaacc ccgccgtccg acccgtccgt 1501 ttgggaactg tcccgttacg ctgcttccgc tgctgacgac ccgcagctgg ctatgaaaat 1561 cttctggtcc tccctccagt gcgcttggta cctgggtgct tcctccgttg ttgctgttac 1621 caccaccgct atggaacgtt acttcgttcg taacggtgtt atcctccagc gtctgggtcc 1681 gccgcagaaa gttaaaggtg aaaccctggt tgctatctcc ttcccggctt accaggaacg 1741 tggtctggaa atgctgctgc gttaccaccc ggaatggctc cagggtgttc cgctgtccat 1801 ggctgttgct gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta 1861 gtgtagatct c //