LOCUS       BBa_K1632033            1871 bp    DNA     linear       06-May-2024
DEFINITION  fim switch[default OFF](Tokyo_Tech/J23119)_rbs_gfp_rbs_lasI
ACCESSION   BBa_K1632033
VERSION     BBa_K1632033.1  
FEATURES             Location/Qualifiers
     misc_feature    84..91
                     /label=Asc1
     misc_feature    92..97
                     /label=Bgl2
     misc_feature    78..83
                     /label=Mlu1
     promoter        98..132
                     /label=J23119 promoter
     misc_feature    133..138
                     /label=BamH1
     misc_feature    139..146
                     /label=Asc1
     misc_feature    147..152
                     /label=Sal1
     promoter        1..432
                     /label=BBa_K1632001
     misc_feature    445..448
                     /label=conserved
     RBS             441..452
                     /label=BBa_B0034
     CDS             459..1178
                     /label=GFP protein
     CDS             459..1178
                     /label=BBa_E0040
     misc_feature    1191..1194
                     /label=conserved
     RBS             1187..1198
                     /label=BBa_B0034
     CDS             1205..1807
                     /label=LasI
     misc_feature    1808..1840
                     /label=LVA
     misc_feature    1847..1871
                     /label=Help:Barcodes
     CDS             1205..1846
                     /label=BBa_C0078
     misc_feature    1187..1871
                     /label=BBa_S03154
ORIGIN
        1 tttgatttaa cttattgata ataaagttaa aaaaacaaat aaatacaaga caattggggc
       61 caaactgtcc atatcatacg cgtggcgcgc cagatctgct agcattatac ctaggactga
      121 gctagctgtc aaggatccgg cgcgccgtcg acgcataaaa atattaaaaa acgacaaaaa
      181 gcatctaact gtttgatatg taaattattt ctattgtaaa ttaatttcac atcacctccg
      241 ctatatgtaa agctaacgtt tctgtggctc gacgcatctt cctcattctt ctctccaaaa
      301 accacctcat gcaatataaa catctataaa taaagataac aatagaatat taagccaaca
      361 aataaactga aaaagtttgt ccgcgatgct ttcctctatg agtcaaaatg gccccaaatg
      421 tttcatcttt tgtactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt
      481 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      541 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
      601 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg
      661 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
      721 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
      781 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
      841 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
      901 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
      961 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     1021 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     1081 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     1141 ggattacaca tggcatggat gaactataca aataataata ctagagaaag aggagaaata
     1201 ctagatgatc gttcagatcg gtcgtcgtga agagttcgac aaaaaactgc tgggtgaaat
     1261 gcacaaactg cgtgctcagg ttttcaaaga acgtaaaggt tgggacgttt ccgttatcga
     1321 cgaaatggaa atcgacggtt acgacgctct gtccccgtac tacatgctga tccaggaaga
     1381 caccccggaa gctcaggttt tcggttgctg gcgtatcttc gacaccaccg gtccgtacat
     1441 gctgaaaaac accttcccgg aactgctgca cggtaaagaa gctccgtgct ccccgcacat
     1501 ctgggaactg tcccgtttcg ctatcaactc cggtcagaaa ggttccctgg gtttctccga
     1561 ctgcaccctg gaagctatgc gtgctctggc tcgttactcc ttgcagaacg acatccagac
     1621 cctggttacc gttaccaccg ttggtgttga aaaaatgatg atccgtgctg gtctggacgt
     1681 ttcccgtttc ggtccgcacc tgaaaatcgg tatcgaacgt gctgttgctc tgcgtatcga
     1741 actgaacgct aaaacccaga tcgctctgta cggtggtgtt ctggttgaac agcgtctggc
     1801 tgtttccgct gctaacgacg aaaactacgc tctggttgct taataactct gatagtgcta
     1861 gtgtagatct c
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