BBa_K1633007 1 BBa_K1633007 GFP siRNA 2015-09-12T11:00:00Z 2015-09-18T08:52:08Z We designed the GFP 122 siRNA and ordered the part from a DNA synthesis company. This part is a short hairpin RNA (shRNA) sequence. When this shRNA sequence is cut by restriction enzyme and then integrated into pcDNA 6.2 vector, this shRNA can play a RNAi function in mammalian cell lines such as HEK293 cell. When the shRNA vector of MOR is transfected into HEK293 cells, the shRNA hairpin structure is cleaved by Dicer into siRNA of MOR and loaded into the RISC. The siRNA-RISC complex targets at GFP mRNA under the guide of siRNA sequence and cleave the GFP mRNA. false false _2050_ 16730 24400 9 false We designed specific GFP siRNAs based on a free software accessible online. This tool can find the best siRNA sequences on target gene GFP to insure the maximum gene-specificity and silencing efficacy. This tool also designs the pair of oligonucleotides needed to generate short hairpin RNAs (shRNAs) in the plasmid. false Chen Xi, Zhou Yu, Jiang Waner, Zhang Peng, Tian Chenfei BBa_K1633007_sequence 1 ttcgttcgactgggacttcaagttttggccactgacgcaagctgaccctgaagttca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z