BBa_K1633008 1 BBa_K1633008 coding sequence to express nSMase 2 protein. 2015-09-12T11:00:00Z 2015-09-16T05:46:23Z Homo Sapiens. The original sequence is from NCBI. NCBI Gene ID is 55512. The sequence codon of this part was optimized. We ordered the sequence from a DNA synthesis company This part is a codon-optimized nSMase 2 gene CDS. When this part is inserted into pcDNA 3.1 vector and transfected into mammalian cells,it can express nSMase2 gene in mammalian cells and stimulate the secretion of exosomal siRNAs from cells. false false _2050_ 16730 24400 9 false There are several Pst I and EcoR I restriction enzyme sites in original nSMase 2 gene sequence. We optimized the gene sequence codon to fulfill the requirements. false Chen Xi, Zhou Yu, Jiang Waner, Zhang Peng, Tian Chenfei BBa_K1633008_sequence 1 agatggtgctgtataccacaccattccccaactcatgcctgagcgccctgcactgcgtgagctgggcactgattttcccctgctattggctggtcgacaggctggccgcttcttttatccctactacctacgagaagcggcagagagctgacgatccatgctgtctccagctgctgtgcaccgcactgtttacacccatctacctggccctgctggtggcatccctgcctttcgcatttctggggttcctgttttggtcccctctccagtctgcccggagaccatacatctattctcgcctggaagataaaggactggcaggaggagcagccctgctgtcagagtggaagggcaccgggccaggaaaaagcttctgctttgcaacagccaacgtgtgcctgctgccagacagcctggcacgagtcaacaatctgttcaacacccaggctagagcaaaggagattggccagaggatccgcaatggggctgcacggccacagatcaaaatctacatcgattcccccacaaatactagcatttccgccgctagttttagctccctggtgtcaccacagggaggcgacggagtggcacgagctgtcccaggaagcatcaagcgaaccgcctccgtggaatacaaaggggatggaggaagacacccaggagacgaggcagcaaacggaccagcttccggagaccctgtggattctagttcacctgaagatgcctgcattgtccggatcggaggagaggaaggaggcagaccccctgaggcagacgatcctgtgccaggaggacaggccaggaatggagctggaggaggaccacgaggacagacccccaaccataatcagcaggacggggattctggaagtctgggctcacctagcgcctccagagaatctctggtgaaggggagggctggacccgacacatctgcaagtggcgagcctggggccaactctaagctgctgtacaaagctagtgtggtcaagaaagctgccgccaggcgccgacggcacccagacgaagccttcgatcatgaggtgagcgcattctttcctgccaatctggacttcctgtgcctccaggaggtctttgacaagcgggctgcaaccaagctgaaagaacagctgcacggctactttgagtatattctgtacgatgtgggagtctatggctgccaggggtgctgttctttcaagtgtctgaacagtggcctgctgtttgcctcacggtaccctatcatggacgtggcttatcattgctacccaaacaaatgtaatgacgatgcactggcctccaagggggccctgttcctgaaagtgcaggtcggatctactcctcaggatcagagaattgtgggatatatcgcttgcacccacctgcatgcaccacaggaggacagtgccatcagatgtggccagctggacctgctccaggattggctggctgacttccgcaagagcactagctcctctagtgccgctaatcccgaggaactggtggcctttgatgtggtctgcggcgatttcaactttgacaattgttcaagcgacgataagctggaacagcagcacagcctgttcacacattaccgagacccttgccggctgggaccaggagaggaaaaaccctgggccatcggaactctgctggataccaacggcctgtatgacgaggacgtgtgcacaccagacaacctccagaaggtcctggaaagtgaggaaggcagaagggagtacctggcctttcccacttcaaagtcctctggacagaagggcaggaaagaactgctgaaaggaaatggccgccgaattgattatatgctgcacgcagaggaaggcctgtgccctgactggaaagccgaggtggaggagttcagcttcatcacacagctgtccggcctgactgaccatctgccagtggccatgcgcctgatggtcagttcaggggaggaagaggcttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z