BBa_K1634005 1 BBa_K1634005 pmyo3 (a promoter in C.elegans) 2015-09-13T11:00:00Z 2015-09-14T12:26:39Z We get Pmyo3 from the C.elegans' genomic DNA by PCR. "myo-3 encodes MHC A, the minor isoform of MHC (myosin heavy chain) that is essential for thick filament formation, and for viability, movement, and embryonic elongation; expressed in body muscle, the somatic sheath cell covering the hermaphrodite gonad, and also expressed in enteric muscle, vulval muscles of the hermaphrodite and the diagonal muscles of the male tail. "(from Wormatlas) Since we want to control the C.elegans' movement,we decided to use pmyo-3 (promoter of myo-3) to construct a plasmid which can drive the expression in the worm???s body muscle. false false _2051_ 0 24399 24399 9 Not in stock false Since we use the restriction enzyme site to do the ligation, we should pay attention to the sequence of Pmyo3, avoiding using the restriction site exits in it. false Xinhong Chen annotation2453687 1 pmyo-3 range2453687 1 1 814 BBa_K1634005_sequence 1 aggaaccgattgatgatttggaaaatccagctttacctgtgtaagaactgaaaagtttcataaccctagggtattcccagttacattccccactggctaacaatagcacccagtttttcatcacctttcttcaaatttctcggcgatttgttaaaaacaaaatttgtgtcccttctctgatatctctatgtctctaaacacaagttcatcggaaaacgaaggagggtaggtgttggttgggctcccgaagtgaaaatagaagagcaagaatagaatattagagagagagtgcagagagggcgggatagctcccgggattccgttttcttcttctttatcttcaacgatgatgtgtgtgcgtgttgtatagattctgttgctcccccacaactcgctccgaaggctcaatacaattcaattgatattggaggagagcctaccggagtgggaggataagaagaaacataagaagaagaagaagaagaagcatgcttctggtttttgatgctatgaaaacggcacaaaaagatgattgaggtcccttttcaataccttctctcatctttcaaatcccattgaaacctaaaacttctcaccacgctttaccattgttctccaaaaacttatagcaatgtctataacttttttatctctgaaaagcagtgttccatttttctttttcctattttatttcaattgtttctcacatttcgtttggattctttgcttgtcaaccagcttcttcttccacttttaccgtctaattttcagggcagggagccatcaaacccacgaccactagatccatgtagaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z