BBa_K1638002
1
T25 domain
T25 domain of CyaA from Bordetella pertussis
2015-05-08T11:00:00Z
2015-05-10T01:14:50Z
asd
This part codes for the T25 domain of the adenylate cyclase, CyaA, from Bordetella pertussis. When associated with the T18 domain of CyaA, the two domains together become active and catalyzes the conversion of ATP to cAMP
false
false
_2055_
26157
26157
9
false
gw
false
Jens Sivk??r Pettersen
annotation2431871
1
BamHI restriction site
range2431871
1
682
687
BBa_K1638000
1
pLac
Lac operator
2015-05-06T11:00:00Z
2015-05-08T01:10:55Z
Coming
bla bla
false
false
_2055_
0
26157
9
Not in stock
false
Coming
false
Jens Sivk??r Pettersen
annotation2431869
1
CAP binding
range2431869
1
55
70
annotation2431867
1
Lac promoter
range2431867
1
88
112
annotation2431868
1
Lac operator
range2431868
1
121
142
BBa_K1638001
1
BBa_K1638001
RBS from pKT25 and pUT18
2015-05-06T11:00:00Z
2015-05-10T01:27:55Z
coming
coming
false
false
_2055_
26157
26157
9
Not in stock
false
coming
false
Jens Sivk??r Pettersen
annotation2431870
1
Shine-Dalgarno
range2431870
1
9
14
BBa_K1638003
1
BBa_K1638003
T25 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> (IPTG inducible)
2015-05-08T11:00:00Z
2015-09-18T10:36:33Z
asd
The T25 domain of CyaA to be used in the bacterial two-hybrid system. The expression of the gene is under control of a lac operator and is induced by IPTG.
When protein-coding genes is suffixed to the T25 and T18 domains of the bacterial two-hybrid system, the interaction of these two proteins can be examined. If the protein associates, T25 and T18 associates too, and convert ATP to cAMP.
false
false
_2055_
26157
26157
9
false
asd
false
Jens Sivk??r Pettersen
component2431917
1
BBa_K1638001
component2431919
1
BBa_K1638002
component2431915
1
BBa_K1638000
annotation2431917
1
BBa_K1638001
range2431917
1
143
161
annotation2431915
1
BBa_K1638000
range2431915
1
1
142
annotation2431919
1
BBa_K1638002
range2431919
1
162
848
BBa_K1638001_sequence
1
tttcacacaggaaacagct
BBa_K1638000_sequence
1
ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaa
BBa_K1638002_sequence
1
atgaccatgcagcaatcgcatcaggctggttacgcaaacgccgccgaccgggagtctggcatccccgcagccgtactcgatggcatcaaggccgtggcgaaggaaaaaaacgccacattgatgttccgcctggtcaacccccattccaccagcctgattgccgaaggggtggccaccaaaggattgggcgtgcacgccaagtcgtccgattgggggttgcaggcgggctacattcccgtcaacccgaatctttccaaactgttcggccgtgcgcccgaggtgatcgcgcgggccgacaacgacgtcaacagcagcctggcgcatggccataccgcggtcgacctgacgctgtcgaaagagcggcttgactatctgcggcaagcgggcctggtcaccggcatggccgatggcgtggtcgcgagcaaccacgcaggctacgagcagttcgagtttcgcgtgaaggaaacctcggacgggcgctatgccgtgcagtatcgccgcaagggcggcgacgatttcgaggcggtcaaggtgatcggcaatgccgccggtattccactgacggcggatatcgacatgttcgccattatgccgcatctgtccaacttccgcgactcggcgcgcagttcggtgaccagcggcgattcggtgaccgattacctggcgcgcacgcggcgggctggatcc
BBa_K1638003_sequence
1
ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgcagcaatcgcatcaggctggttacgcaaacgccgccgaccgggagtctggcatccccgcagccgtactcgatggcatcaaggccgtggcgaaggaaaaaaacgccacattgatgttccgcctggtcaacccccattccaccagcctgattgccgaaggggtggccaccaaaggattgggcgtgcacgccaagtcgtccgattgggggttgcaggcgggctacattcccgtcaacccgaatctttccaaactgttcggccgtgcgcccgaggtgatcgcgcgggccgacaacgacgtcaacagcagcctggcgcatggccataccgcggtcgacctgacgctgtcgaaagagcggcttgactatctgcggcaagcgggcctggtcaccggcatggccgatggcgtggtcgcgagcaaccacgcaggctacgagcagttcgagtttcgcgtgaaggaaacctcggacgggcgctatgccgtgcagtatcgccgcaagggcggcgacgatttcgaggcggtcaaggtgatcggcaatgccgccggtattccactgacggcggatatcgacatgttcgccattatgccgcatctgtccaacttccgcgactcggcgcgcagttcggtgaccagcggcgattcggtgaccgattacctggcgcgcacgcggcgggctggatcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z