LOCUS       BBa_K1638010            1729 bp    DNA     linear       26-Jun-2024
DEFINITION  T25 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> with 
            GFP reporter
ACCESSION   BBa_K1638010
VERSION     BBa_K1638010.1  
FEATURES             Location/Qualifiers
     promoter        88..112
                     /label=Lac promoter
     misc_feature    121..142
                     /label=Lac operator
     misc_feature    55..70
                     /label=CAP binding
     promoter        1..142
                     /label=BBa_K1638000
     misc_feature    151..156
                     /label=Shine-Dalgarno
     RBS             143..161
                     /label=BBa_K1638001
     misc_feature    843..848
                     /label=BamHI restriction site
     CDS             162..848
                     /label=BBa_K1638002
     misc_feature    1..848
                     /label=BBa_K1638003
     misc_feature    849..1729
                     /label=BBa_K1638008
ORIGIN
        1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
       61 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg cagcaatcgc
      181 atcaggctgg ttacgcaaac gccgccgacc gggagtctgg catccccgca gccgtactcg
      241 atggcatcaa ggccgtggcg aaggaaaaaa acgccacatt gatgttccgc ctggtcaacc
      301 cccattccac cagcctgatt gccgaagggg tggccaccaa aggattgggc gtgcacgcca
      361 agtcgtccga ttgggggttg caggcgggct acattcccgt caacccgaat ctttccaaac
      421 tgttcggccg tgcgcccgag gtgatcgcgc gggccgacaa cgacgtcaac agcagcctgg
      481 cgcatggcca taccgcggtc gacctgacgc tgtcgaaaga gcggcttgac tatctgcggc
      541 aagcgggcct ggtcaccggc atggccgatg gcgtggtcgc gagcaaccac gcaggctacg
      601 agcagttcga gtttcgcgtg aaggaaacct cggacgggcg ctatgccgtg cagtatcgcc
      661 gcaagggcgg cgacgatttc gaggcggtca aggtgatcgg caatgccgcc ggtattccac
      721 tgacggcgga tatcgacatg ttcgccatta tgccgcatct gtccaacttc cgcgactcgg
      781 cgcgcagttc ggtgaccagc ggcgattcgg tgaccgatta cctggcgcgc acgcggcggg
      841 ctggatccga aaatttgtat tttcaatctg gtatgcgtaa aggagaagaa cttttcactg
      901 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
      961 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     1021 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat
     1081 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg
     1141 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
     1201 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
     1261 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
     1321 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
     1381 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
     1441 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
     1501 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
     1561 cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga
     1621 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1681 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//