LOCUS BBa_K1638010 1729 bp DNA linear 22-Nov-2024
DEFINITION T25 domain of cyaA from Bordetella pertussis with
GFP reporter
ACCESSION BBa_K1638010
VERSION BBa_K1638010.1
FEATURES Location/Qualifiers
promoter 88..112
/label=Lac promoter
misc_feature 121..142
/label=Lac operator
misc_feature 55..70
/label=CAP binding
promoter 1..142
/label=BBa_K1638000
misc_feature 151..156
/label=Shine-Dalgarno
RBS 143..161
/label=BBa_K1638001
misc_feature 843..848
/label=BamHI restriction site
CDS 162..848
/label=BBa_K1638002
misc_feature 1..848
/label=BBa_K1638003
misc_feature 849..1729
/label=BBa_K1638008
ORIGIN
1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
61 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg cagcaatcgc
181 atcaggctgg ttacgcaaac gccgccgacc gggagtctgg catccccgca gccgtactcg
241 atggcatcaa ggccgtggcg aaggaaaaaa acgccacatt gatgttccgc ctggtcaacc
301 cccattccac cagcctgatt gccgaagggg tggccaccaa aggattgggc gtgcacgcca
361 agtcgtccga ttgggggttg caggcgggct acattcccgt caacccgaat ctttccaaac
421 tgttcggccg tgcgcccgag gtgatcgcgc gggccgacaa cgacgtcaac agcagcctgg
481 cgcatggcca taccgcggtc gacctgacgc tgtcgaaaga gcggcttgac tatctgcggc
541 aagcgggcct ggtcaccggc atggccgatg gcgtggtcgc gagcaaccac gcaggctacg
601 agcagttcga gtttcgcgtg aaggaaacct cggacgggcg ctatgccgtg cagtatcgcc
661 gcaagggcgg cgacgatttc gaggcggtca aggtgatcgg caatgccgcc ggtattccac
721 tgacggcgga tatcgacatg ttcgccatta tgccgcatct gtccaacttc cgcgactcgg
781 cgcgcagttc ggtgaccagc ggcgattcgg tgaccgatta cctggcgcgc acgcggcggg
841 ctggatccga aaatttgtat tttcaatctg gtatgcgtaa aggagaagaa cttttcactg
901 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
961 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
1021 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat
1081 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg
1141 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
1201 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
1261 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
1321 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
1381 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
1441 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
1501 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
1561 cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga
1621 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
1681 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
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