BBa_K1638015 1 BBa_K1638015 hTrx scaffold for peptide aptamers with 3xFLAG-tag and flexible linker 2015-08-11T11:00:00Z 2015-08-12T02:01:58Z GenBank: AY004872.1 coming false false _2055_ 26157 26157 9 false coming false Jens Sivk??r Pettersen component2435204 1 BBa_K1638016 component2435207 1 BBa_K1638014 annotation2435204 1 BBa_K1638016 range2435204 1 1 24 annotation2435207 1 BBa_K1638014 range2435207 1 25 414 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2002 1 -10 range2002 1 43 48 annotation1999 1 lac O1 range1999 1 3 19 annotation2000 1 -35 range2000 1 20 25 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2001 1 lac O1 range2001 1 26 42 BBa_K1638018 1 BBa_K1638018 hTrx-based scaffold fused to T18 through a flexible linker 2015-08-11T11:00:00Z 2015-09-09T08:09:37Z . coming false false _2055_ 26157 26157 9 false . false Jens Sivk??r Pettersen component2447808 1 BBa_R0011 component2447815 1 BBa_K1638004 component2447820 1 BBa_K1638015 component2447813 1 BBa_K1638017 annotation2447820 1 BBa_K1638015 range2447820 1 630 1043 annotation2447808 1 BBa_R0011 range2447808 1 1 54 annotation2447815 1 BBa_K1638004 range2447815 1 75 629 annotation2447813 1 BBa_K1638017 range2447813 1 56 74 BBa_K1638004 1 T18 domain T18 domain of CyaA from Bordetella pertussis 2015-05-09T11:00:00Z 2015-05-10T01:14:12Z .. This part codes for the T18 domain of the adenylate cyclase, CyaA, from Bordetella pertussis. When associated with the T25 domain of CyaA, the two domains together become active and catalyzes the conversion of ATP to cAMP. false false _2055_ 26157 26157 9 false .. false Jens Sivk??r Pettersen annotation2431921 1 BamHI restriction site range2431921 1 550 555 BBa_K1638016 1 BBa_K1638016 8 aa linker without BamHI restriction site and TEV recognition site 2015-08-11T11:00:00Z 2015-08-12T02:01:37Z . This part is similar to the 10 aa linker with BamHI restriction site and TEV recognition site, but without the initial BamHI restriction site. false false _2055_ 26157 26157 9 false . false Jens Sivk??r Pettersen BBa_K1638014 1 BBa_K1638014 hTrx scaffold for peptide aptamers with 3xFLAG-tag 2015-08-11T11:00:00Z 2015-08-12T10:21:37Z GenBank: AY004872.1 coming false false _2055_ 26157 26157 9 false .. false Jens Sivk??r Pettersen annotation2435120 1 3xFLAG range2435120 1 318 390 annotation2435121 1 Xho res. site range2435121 1 100 105 BBa_K1638017 1 BBa_K1638017 RBS from pKT25 and pUT18C with consensus Shine-Dalgarno 2015-08-11T11:00:00Z 2015-08-12T02:19:48Z . coming false false _2055_ 26157 26157 9 false . false Jens Sivk??r Pettersen BBa_K1638018_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatttcacacaggaggcagctatgaccatgattacgccaagcttagccgccagcgaggccacgggcggcctggatcgcgaacgcatcgacttgttgtggaaaatcgctcgcgccggcgcccgttccgcagtgggcaccgaggcgcgtcgccagttccgctacgacggcgacatgaatatcggcgtgatcaccgatttcgagctggaagtgcgcaatgcgctgaacaggcgggcgcacgccgtcggcgcgcaggacgtggtccagcatggcactgagcagaacaatcctttcccggaggcagatgagaagattttcgtcgtatcggccaccggtgaaagccagatgctcacgcgcgggcaactgaaggaatacattggccagcagcgcggcgagggctatgtcttctacgagaaccgtgcatacggcgtggcggggaaaagcctgttcgacgatgggctgggagccgcgcccggcgtgccgagcggacgttcgaagttctcgccggatgtactggaaacggtgccggcgtcacccggattgcggcggccgtcgctgggcgcagtggaacgcggatccgaaaatttgtattttcaatctggtaccatggtgaaacaaattgagtccaagaccgcctttcaagaggcactggacgccgcgggtgacaaactcgtggtcgttgatttcagcgccacctggggcctcgagggcaagatgattaaaccgttttttcattcgctgtctgaaaagtattcaaacgtgattttcctggaggttgatgtggacgacagccaggatgtggccagcgaaagcgaagttaaaagcatgcctacatttcagtttttcaaaaaaggtcagaaagttggcgaatttagtggcgccaataaagaaaaattggaggctaccatcaacgaattggtcatggattacaaagaccacgatggggactataaagaccatgacattgattataaagatgacgacgataagtag BBa_K1638004_sequence 1 atgaccatgattacgccaagcttagccgccagcgaggccacgggcggcctggatcgcgaacgcatcgacttgttgtggaaaatcgctcgcgccggcgcccgttccgcagtgggcaccgaggcgcgtcgccagttccgctacgacggcgacatgaatatcggcgtgatcaccgatttcgagctggaagtgcgcaatgcgctgaacaggcgggcgcacgccgtcggcgcgcaggacgtggtccagcatggcactgagcagaacaatcctttcccggaggcagatgagaagattttcgtcgtatcggccaccggtgaaagccagatgctcacgcgcgggcaactgaaggaatacattggccagcagcgcggcgagggctatgtcttctacgagaaccgtgcatacggcgtggcggggaaaagcctgttcgacgatgggctgggagccgcgcccggcgtgccgagcggacgttcgaagttctcgccggatgtactggaaacggtgccggcgtcacccggattgcggcggccgtcgctgggcgcagtggaacgcggatcc BBa_K1638014_sequence 1 accatggtgaaacaaattgagtccaagaccgcctttcaagaggcactggacgccgcgggtgacaaactcgtggtcgttgatttcagcgccacctggggcctcgagggcaagatgattaaaccgttttttcattcgctgtctgaaaagtattcaaacgtgattttcctggaggttgatgtggacgacagccaggatgtggccagcgaaagcgaagttaaaagcatgcctacatttcagtttttcaaaaaaggtcagaaagttggcgaatttagtggcgccaataaagaaaaattggaggctaccatcaacgaattggtcatggattacaaagaccacgatggggactataaagaccatgacattgattataaagatgacgacgataagtag BBa_K1638016_sequence 1 gaaaatttgtattttcaatctggt BBa_K1638015_sequence 1 gaaaatttgtattttcaatctggtaccatggtgaaacaaattgagtccaagaccgcctttcaagaggcactggacgccgcgggtgacaaactcgtggtcgttgatttcagcgccacctggggcctcgagggcaagatgattaaaccgttttttcattcgctgtctgaaaagtattcaaacgtgattttcctggaggttgatgtggacgacagccaggatgtggccagcgaaagcgaagttaaaagcatgcctacatttcagtttttcaaaaaaggtcagaaagttggcgaatttagtggcgccaataaagaaaaattggaggctaccatcaacgaattggtcatggattacaaagaccacgatggggactataaagaccatgacattgattataaagatgacgacgataagtag BBa_K1638017_sequence 1 tttcacacaggaggcagct BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z