LOCUS       BBa_K1638026            1724 bp    DNA     linear       28-Mar-2025
DEFINITION  Yhbj fused to T25 domain of CyaA
ACCESSION   BBa_K1638026
VERSION     BBa_K1638026.1  
FEATURES             Location/Qualifiers
     promoter        88..112
                     /label=Lac promoter
     misc_feature    121..142
                     /label=Lac operator
     misc_feature    55..70
                     /label=CAP binding
     promoter        1..142
                     /label=BBa_K1638000
     misc_feature    151..156
                     /label=Shine-Dalgarno
     RBS             143..161
                     /label=BBa_K1638001
     misc_feature    843..848
                     /label=BamHI restriction site
     CDS             162..848
                     /label=BBa_K1638002
     misc_feature    1..848
                     /label=BBa_K1638003
     misc_feature    849..872
                     /label=BBa_K1638016
     CDS             873..1724
                     /label=BBa_K1638021
ORIGIN
        1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
       61 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg cagcaatcgc
      181 atcaggctgg ttacgcaaac gccgccgacc gggagtctgg catccccgca gccgtactcg
      241 atggcatcaa ggccgtggcg aaggaaaaaa acgccacatt gatgttccgc ctggtcaacc
      301 cccattccac cagcctgatt gccgaagggg tggccaccaa aggattgggc gtgcacgcca
      361 agtcgtccga ttgggggttg caggcgggct acattcccgt caacccgaat ctttccaaac
      421 tgttcggccg tgcgcccgag gtgatcgcgc gggccgacaa cgacgtcaac agcagcctgg
      481 cgcatggcca taccgcggtc gacctgacgc tgtcgaaaga gcggcttgac tatctgcggc
      541 aagcgggcct ggtcaccggc atggccgatg gcgtggtcgc gagcaaccac gcaggctacg
      601 agcagttcga gtttcgcgtg aaggaaacct cggacgggcg ctatgccgtg cagtatcgcc
      661 gcaagggcgg cgacgatttc gaggcggtca aggtgatcgg caatgccgcc ggtattccac
      721 tgacggcgga tatcgacatg ttcgccatta tgccgcatct gtccaacttc cgcgactcgg
      781 cgcgcagttc ggtgaccagc ggcgattcgg tgaccgatta cctggcgcgc acgcggcggg
      841 ctggatccga aaatttgtat tttcaatctg gtatggtcct gatgattgtt agtgggcgca
      901 gcggcagcgg caaaagcgtt gctctgcgcg ctctggagga tatgggtttt tattgcgtcg
      961 acaacctgcc agtggtcctg ctgcccgatc tggcgcgtac cctggcggat cgcgaaattt
     1021 ctgctgcggt gagtatcgac gttcgcaaca tgccggaaag cccagaaatt ttcgaacagg
     1081 caatgagcaa cctgccagac gcatttagcc ctcaactttt attcctggat gcggatcgta
     1141 acaccttgat tcgtcgctat tccgacacgc gtcgcttgca tccgctgtcg agcaaaaact
     1201 tgtccttgga atcagcgatt gacaaagaat ctgatctgct cgaaccgctg cgcagccgcg
     1261 cggacctgat cgtggacacc agtgaaatga gcgtgcacga gttagcggaa atgctgcgta
     1321 cccgtctgct gggaaagcgc gaacgcgagc tgaccatggt ttttgaatct tttggtttca
     1381 agcatggcat ccctattgac gcggattatg tcttcgatgt ccgtttttta ccgaacccac
     1441 attgggaccc gaaactgcgt cccatgaccg gactggataa gccggttgcg gccttcttgg
     1501 atcgccatac cgaggtgcat aactttattt atcagacgcg ctcttatctg gagctgtggt
     1561 taccgatgct ggaaaccaat aaccgttcct acctgactgt tgccattggt tgcactggag
     1621 gaaaacatcg cagtgtctat atcgccgaac agttagcaga ttatttccgc tcgcgcggca
     1681 agaacgttca gagccgtcat cgtacacttg aaaagcgcaa gcca
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