BBa_K1638002 1 T25 domain T25 domain of CyaA from Bordetella pertussis 2015-05-08T11:00:00Z 2015-05-10T01:14:50Z asd This part codes for the T25 domain of the adenylate cyclase, CyaA, from Bordetella pertussis. When associated with the T18 domain of CyaA, the two domains together become active and catalyzes the conversion of ATP to cAMP false false _2055_ 26157 26157 9 false gw false Jens Sivk??r Pettersen annotation2431871 1 BamHI restriction site range2431871 1 682 687 BBa_K1638003 1 BBa_K1638003 T25 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> (IPTG inducible) 2015-05-08T11:00:00Z 2015-09-18T10:36:33Z asd The T25 domain of CyaA to be used in the bacterial two-hybrid system. The expression of the gene is under control of a lac operator and is induced by IPTG. When protein-coding genes is suffixed to the T25 and T18 domains of the bacterial two-hybrid system, the interaction of these two proteins can be examined. If the protein associates, T25 and T18 associates too, and convert ATP to cAMP. false false _2055_ 26157 26157 9 false asd false Jens Sivk??r Pettersen component2431915 1 BBa_K1638000 component2431917 1 BBa_K1638001 component2431919 1 BBa_K1638002 annotation2431919 1 BBa_K1638002 range2431919 1 162 848 annotation2431915 1 BBa_K1638000 range2431915 1 1 142 annotation2431917 1 BBa_K1638001 range2431917 1 143 161 BBa_K1638000 1 pLac Lac operator 2015-05-06T11:00:00Z 2015-05-08T01:10:55Z Coming bla bla false false _2055_ 0 26157 9 Not in stock false Coming false Jens Sivk??r Pettersen annotation2431867 1 Lac promoter range2431867 1 88 112 annotation2431868 1 Lac operator range2431868 1 121 142 annotation2431869 1 CAP binding range2431869 1 55 70 BBa_K1638001 1 BBa_K1638001 RBS from pKT25 and pUT18 2015-05-06T11:00:00Z 2015-05-10T01:27:55Z coming coming false false _2055_ 26157 26157 9 Not in stock false coming false Jens Sivk??r Pettersen annotation2431870 1 Shine-Dalgarno range2431870 1 9 14 BBa_K1638022 1 BBa_K1638022 Endoribonuclease ybeY 2015-08-12T11:00:00Z 2015-09-10T12:02:30Z GenBank: AAB40861.1 coming true false _2055_ 26157 26157 9 false . false Jens Sivk??r Pettersen BBa_K1638016 1 BBa_K1638016 8 aa linker without BamHI restriction site and TEV recognition site 2015-08-11T11:00:00Z 2015-08-12T02:01:37Z . This part is similar to the 10 aa linker with BamHI restriction site and TEV recognition site, but without the initial BamHI restriction site. false false _2055_ 26157 26157 9 false . false Jens Sivk??r Pettersen BBa_K1638027 1 BBa_K1638027 YbeY fused to T25 domain of CyaA 2015-08-12T11:00:00Z 2015-09-11T04:23:14Z . coming true false _2055_ 26157 26157 9 false . false Jens Sivk??r Pettersen component2435489 1 BBa_K1638003 component2435491 1 BBa_K1638022 component2435490 1 BBa_K1638016 annotation2435491 1 BBa_K1638022 range2435491 1 873 1337 annotation2435490 1 BBa_K1638016 range2435490 1 849 872 annotation2435489 1 BBa_K1638003 range2435489 1 1 848 BBa_K1638001_sequence 1 tttcacacaggaaacagct BBa_K1638000_sequence 1 ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaa BBa_K1638022_sequence 1 atgagccaagtgatcctggatctgcagttggcttgcgaggacaattccggcctgccggaagaatctcaatttcagacatggttgaatgcggttattcctcaatttcaagaagaatcggaggtcacgatccgcgtagtggatacagctgaaagtcattccctgaatttaacgtaccgtggcaaggataaaccgaccaatgttctcagtttcccattcgaagtaccgccaggaatggaaatgagcctgctgggtgatttggtcatctgccgtcaggtggttgagaaggaagcgcaggaacagggcaaaccgttagaagcgcactgggcgcatatggtcgttcacgggtccctccatctgctcggctacgatcacattgaagatgatgaagccgaagaaatggaggcgttagagaccgaaatcatgcttgcgctgggctacgaggatccgtatattgcggaaaaagag BBa_K1638016_sequence 1 gaaaatttgtattttcaatctggt BBa_K1638027_sequence 1 ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgcagcaatcgcatcaggctggttacgcaaacgccgccgaccgggagtctggcatccccgcagccgtactcgatggcatcaaggccgtggcgaaggaaaaaaacgccacattgatgttccgcctggtcaacccccattccaccagcctgattgccgaaggggtggccaccaaaggattgggcgtgcacgccaagtcgtccgattgggggttgcaggcgggctacattcccgtcaacccgaatctttccaaactgttcggccgtgcgcccgaggtgatcgcgcgggccgacaacgacgtcaacagcagcctggcgcatggccataccgcggtcgacctgacgctgtcgaaagagcggcttgactatctgcggcaagcgggcctggtcaccggcatggccgatggcgtggtcgcgagcaaccacgcaggctacgagcagttcgagtttcgcgtgaaggaaacctcggacgggcgctatgccgtgcagtatcgccgcaagggcggcgacgatttcgaggcggtcaaggtgatcggcaatgccgccggtattccactgacggcggatatcgacatgttcgccattatgccgcatctgtccaacttccgcgactcggcgcgcagttcggtgaccagcggcgattcggtgaccgattacctggcgcgcacgcggcgggctggatccgaaaatttgtattttcaatctggtatgagccaagtgatcctggatctgcagttggcttgcgaggacaattccggcctgccggaagaatctcaatttcagacatggttgaatgcggttattcctcaatttcaagaagaatcggaggtcacgatccgcgtagtggatacagctgaaagtcattccctgaatttaacgtaccgtggcaaggataaaccgaccaatgttctcagtttcccattcgaagtaccgccaggaatggaaatgagcctgctgggtgatttggtcatctgccgtcaggtggttgagaaggaagcgcaggaacagggcaaaccgttagaagcgcactgggcgcatatggtcgttcacgggtccctccatctgctcggctacgatcacattgaagatgatgaagccgaagaaatggaggcgttagagaccgaaatcatgcttgcgctgggctacgaggatccgtatattgcggaaaaagag BBa_K1638002_sequence 1 atgaccatgcagcaatcgcatcaggctggttacgcaaacgccgccgaccgggagtctggcatccccgcagccgtactcgatggcatcaaggccgtggcgaaggaaaaaaacgccacattgatgttccgcctggtcaacccccattccaccagcctgattgccgaaggggtggccaccaaaggattgggcgtgcacgccaagtcgtccgattgggggttgcaggcgggctacattcccgtcaacccgaatctttccaaactgttcggccgtgcgcccgaggtgatcgcgcgggccgacaacgacgtcaacagcagcctggcgcatggccataccgcggtcgacctgacgctgtcgaaagagcggcttgactatctgcggcaagcgggcctggtcaccggcatggccgatggcgtggtcgcgagcaaccacgcaggctacgagcagttcgagtttcgcgtgaaggaaacctcggacgggcgctatgccgtgcagtatcgccgcaagggcggcgacgatttcgaggcggtcaaggtgatcggcaatgccgccggtattccactgacggcggatatcgacatgttcgccattatgccgcatctgtccaacttccgcgactcggcgcgcagttcggtgaccagcggcgattcggtgaccgattacctggcgcgcacgcggcgggctggatcc BBa_K1638003_sequence 1 ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgcagcaatcgcatcaggctggttacgcaaacgccgccgaccgggagtctggcatccccgcagccgtactcgatggcatcaaggccgtggcgaaggaaaaaaacgccacattgatgttccgcctggtcaacccccattccaccagcctgattgccgaaggggtggccaccaaaggattgggcgtgcacgccaagtcgtccgattgggggttgcaggcgggctacattcccgtcaacccgaatctttccaaactgttcggccgtgcgcccgaggtgatcgcgcgggccgacaacgacgtcaacagcagcctggcgcatggccataccgcggtcgacctgacgctgtcgaaagagcggcttgactatctgcggcaagcgggcctggtcaccggcatggccgatggcgtggtcgcgagcaaccacgcaggctacgagcagttcgagtttcgcgtgaaggaaacctcggacgggcgctatgccgtgcagtatcgccgcaagggcggcgacgatttcgaggcggtcaaggtgatcggcaatgccgccggtattccactgacggcggatatcgacatgttcgccattatgccgcatctgtccaacttccgcgactcggcgcgcagttcggtgaccagcggcgattcggtgaccgattacctggcgcgcacgcggcgggctggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z