BBa_K1638030
type: ComponentDefinition
persistent identity: BBa K1638030
display id: BBa_K1638030
version: 1
was generated by: iGEM to SBOL conversion
description: Leucine zipper fused to T25 domain of cyaA with cAMP-induced RFP generator
created: 2015-08-17T11:00:00Z
modified: 2015-09-18T10:41:49Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: coming
discontinued: false
dominant: false
experience: experience/None
group u list: _2055_
m user id: 26157
owner id: 26157
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Jens Sivk??r Pettersen
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1638013
component: BBa_K861173
sequence annotation: BBa_K861173
sequence annotation: BBa_K1638013
sequence: https://synbiohub.org/public/igem/BBa_K1638030_sequence/1

BBa_K1638013
type: Component
persistent identity: component2473125
display id: component2473125
version: 1
top level: BBa_K1638030
definition: BBa_K1638013
access: public

BBa_K861173
type: Component
persistent identity: component2473112
display id: component2473112
version: 1
top level: BBa_K1638030
definition: BBa_K861173
access: public

BBa_K861173
type: SequenceAnnotation
persistent identity: annotation2473112
display id: annotation2473112
version: 1
top level: BBa_K1638030
location: https://synbiohub.org/public/igem/BBa_K1638030/annotation2473112/range2473112/1
component: BBa_K861173

https://synbiohub.org/public/igem/BBa_K1638030/annotation2473112/range2473112/1
type: Range
persistent identity: range2473112
display id: range2473112
version: 1
top level: BBa_K1638030
direction: direction/reverse
start: 1
end: 1000
orientation: inline

BBa_K1638013
type: SequenceAnnotation
persistent identity: annotation2473125
display id: annotation2473125
version: 1
top level: BBa_K1638030
location: https://synbiohub.org/public/igem/BBa_K1638030/annotation2473125/range2473125/1
component: BBa_K1638013

https://synbiohub.org/public/igem/BBa_K1638030/annotation2473125/range2473125/1
type: Range
persistent identity: range2473125
display id: range2473125
version: 1
top level: BBa_K1638030
direction: direction/reverse
start: 1009
end: 1976
orientation: inline

BBa_K861173
type: ComponentDefinition
persistent identity: BBa K861173
display id: BBa_K861173
version: 1
was generated by: iGEM to SBOL conversion
description: mRFP generator controlled by PcstA (glucose-repressible promoter)
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:36Z
mutable provenance: Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lambda cI is controlled by promoter PcstA,which can be activated by CRP-cAMP complex.therefore protein lambda cI will be expressed under low glucose concentration.This generator contains a RFP after the promoter as a reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Kuanwei Sheng, Xian Xia
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_K118011
component: BBa_I13507
sequence annotation: BBa_K118011
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K861173_sequence/1

BBa_K118011
type: Component
persistent identity: component2193732
display id: component2193732
version: 1
top level: BBa_K861173
definition: BBa_K118011
access: public

BBa_I13507
type: Component
persistent identity: component2193744
display id: component2193744
version: 1
top level: BBa_K861173
definition: BBa_I13507
access: public

BBa_K118011
type: SequenceAnnotation
persistent identity: annotation2193732
display id: annotation2193732
version: 1
top level: BBa_K861173
location: https://synbiohub.org/public/igem/BBa_K861173/annotation2193732/range2193732/1
component: BBa_K118011

https://synbiohub.org/public/igem/BBa_K861173/annotation2193732/range2193732/1
type: Range
persistent identity: range2193732
display id: range2193732
version: 1
top level: BBa_K861173
direction: direction/reverse
start: 1
end: 131
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2193744
display id: annotation2193744
version: 1
top level: BBa_K861173
location: https://synbiohub.org/public/igem/BBa_K861173/annotation2193744/range2193744/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K861173/annotation2193744/range2193744/1
type: Range
persistent identity: range2193744
display id: range2193744
version: 1
top level: BBa_K861173
direction: direction/reverse
start: 140
end: 1000
orientation: inline

BBa_K1638013
type: ComponentDefinition
persistent identity: BBa K1638013
display id: BBa_K1638013
version: 1
was generated by: iGEM to SBOL conversion
description: Leucine zipper fused to T25 domain of cyaA from Bordetella pertussis
created: 2015-05-21T11:00:00Z
modified: 2015-09-18T10:41:12Z
mutable provenance: ..
owned by: james
owned by: myers
mutable description: ..
discontinued: false
dominant: false
experience: experience/None
group u list: _2055_
m user id: 26157
owner id: 26157
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: ..
star: false
creator: Jens Sivk??r Pettersen
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1638003
component: BBa_S05273
sequence annotation: BBa_K1638003
sequence annotation: BBa_S05273
sequence: https://synbiohub.org/public/igem/BBa_K1638013_sequence/1

BBa_K1638003
type: Component
persistent identity: component2473040
display id: component2473040
version: 1
top level: BBa_K1638013
definition: BBa_K1638003
access: public

BBa_S05273
type: Component
persistent identity: component2473043
display id: component2473043
version: 1
top level: BBa_K1638013
definition: BBa_S05273
access: public

BBa_K1638003
type: SequenceAnnotation
persistent identity: annotation2473040
display id: annotation2473040
version: 1
top level: BBa_K1638013
location: https://synbiohub.org/public/igem/BBa_K1638013/annotation2473040/range2473040/1
component: BBa_K1638003

https://synbiohub.org/public/igem/BBa_K1638013/annotation2473040/range2473040/1
type: Range
persistent identity: range2473040
display id: range2473040
version: 1
top level: BBa_K1638013
direction: direction/reverse
start: 1
end: 848
orientation: inline

BBa_S05273
type: SequenceAnnotation
persistent identity: annotation2473043
display id: annotation2473043
version: 1
top level: BBa_K1638013
location: https://synbiohub.org/public/igem/BBa_K1638013/annotation2473043/range2473043/1
component: BBa_S05273

https://synbiohub.org/public/igem/BBa_K1638013/annotation2473043/range2473043/1
type: Range
persistent identity: range2473043
display id: range2473043
version: 1
top level: BBa_K1638013
direction: direction/reverse
start: 849
end: 968
orientation: inline

BBa_I13507
type: ComponentDefinition
persistent identity: BBa I13507
display id: BBa_I13507
version: 1
was generated by: iGEM to SBOL conversion
description: Screening plasmid intermediate
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Built by Josh as an intermediate in screening plasmid construction.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13507_sequence/1

BBa_B0034
type: Component
persistent identity: component1524815
display id: component1524815
version: 1
top level: BBa_I13507
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1524828
display id: component1524828
version: 1
top level: BBa_I13507
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component1524822
display id: component1524822
version: 1
top level: BBa_I13507
definition: mRFP1
access: public

BBa_B0012
type: Component
persistent identity: component1524838
display id: component1524838
version: 1
top level: BBa_I13507
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1524815
display id: annotation1524815
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
type: Range
persistent identity: range1524815
display id: range1524815
version: 1
top level: BBa_I13507
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1524822
display id: annotation1524822
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
type: Range
persistent identity: range1524822
display id: range1524822
version: 1
top level: BBa_I13507
direction: direction/forward
start: 19
end: 699
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1524828
display id: annotation1524828
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
type: Range
persistent identity: range1524828
display id: range1524828
version: 1
top level: BBa_I13507
direction: direction/forward
start: 733
end: 812
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1524838
display id: annotation1524838
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
type: Range
persistent identity: range1524838
display id: range1524838
version: 1
top level: BBa_I13507
direction: direction/forward
start: 821
end: 861
orientation: inline

BBa_K118011
type: ComponentDefinition
persistent identity: BBa K118011
display id: BBa_K118011
version: 1
was generated by: iGEM to SBOL conversion
description: PcstA (glucose-repressible promoter)
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: ''Eschaerichia coli'' JM109 genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.)
discontinued: false
dominant: true
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cAMP receptor protein binding site
sequence annotation: RNA polymerase binding site
sequence: https://synbiohub.org/public/igem/BBa_K118011_sequence/1

cAMP receptor protein binding site
type: SequenceAnnotation
persistent identity: annotation1972435
display id: annotation1972435
version: 1
top level: BBa_K118011
location: https://synbiohub.org/public/igem/BBa_K118011/annotation1972435/range1972435/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K118011/annotation1972435/range1972435/1
type: Range
persistent identity: range1972435
display id: range1972435
version: 1
top level: BBa_K118011
direction: direction/forward
start: 1
end: 42
orientation: inline

RNA polymerase binding site
type: SequenceAnnotation
persistent identity: annotation1972436
display id: annotation1972436
version: 1
top level: BBa_K118011
location: https://synbiohub.org/public/igem/BBa_K118011/annotation1972436/range1972436/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K118011/annotation1972436/range1972436/1
type: Range
persistent identity: range1972436
display id: range1972436
version: 1
top level: BBa_K118011
direction: direction/forward
start: 101
end: 131
orientation: inline

BBa_K1638003
type: ComponentDefinition
persistent identity: BBa K1638003
display id: BBa_K1638003
version: 1
was generated by: iGEM to SBOL conversion
description: T25 domain of cyaA from Bordetella pertussis (IPTG inducible)
created: 2015-05-08T11:00:00Z
modified: 2015-09-18T10:36:33Z
mutable provenance: asd
owned by: james
owned by: myers
mutable description: The T25 domain of CyaA to be used in the bacterial two-hybrid system. The expression of the gene is under control of a lac operator and is induced by IPTG. When protein-coding genes is suffixed to the T25 and T18 domains of the bacterial two-hybrid system, the interaction of these two proteins can be examined. If the protein associates, T25 and T18 associates too, and convert ATP to cAMP.
discontinued: false
dominant: false
experience: experience/None
group u list: _2055_
m user id: 26157
owner id: 26157
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: asd
star: false
creator: Jens Sivk??r Pettersen
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1638000
component: BBa_K1638002
component: BBa_K1638001
sequence annotation: BBa_K1638000
sequence annotation: BBa_K1638001
sequence annotation: BBa_K1638002
sequence: https://synbiohub.org/public/igem/BBa_K1638003_sequence/1

BBa_K1638000
type: Component
persistent identity: component2431915
display id: component2431915
version: 1
top level: BBa_K1638003
definition: pLac
access: public

BBa_K1638002
type: Component
persistent identity: component2431919
display id: component2431919
version: 1
top level: BBa_K1638003
definition: T25 domain
access: public

BBa_K1638001
type: Component
persistent identity: component2431917
display id: component2431917
version: 1
top level: BBa_K1638003
definition: BBa_K1638001
access: public

BBa_K1638000
type: SequenceAnnotation
persistent identity: annotation2431915
display id: annotation2431915
version: 1
top level: BBa_K1638003
location: https://synbiohub.org/public/igem/BBa_K1638003/annotation2431915/range2431915/1
component: BBa_K1638000

https://synbiohub.org/public/igem/BBa_K1638003/annotation2431915/range2431915/1
type: Range
persistent identity: range2431915
display id: range2431915
version: 1
top level: BBa_K1638003
direction: direction/reverse
start: 1
end: 142
orientation: inline

BBa_K1638001
type: SequenceAnnotation
persistent identity: annotation2431917
display id: annotation2431917
version: 1
top level: BBa_K1638003
location: https://synbiohub.org/public/igem/BBa_K1638003/annotation2431917/range2431917/1
component: BBa_K1638001

https://synbiohub.org/public/igem/BBa_K1638003/annotation2431917/range2431917/1
type: Range
persistent identity: range2431917
display id: range2431917
version: 1
top level: BBa_K1638003
direction: direction/reverse
start: 143
end: 161
orientation: inline

BBa_K1638002
type: SequenceAnnotation
persistent identity: annotation2431919
display id: annotation2431919
version: 1
top level: BBa_K1638003
location: https://synbiohub.org/public/igem/BBa_K1638003/annotation2431919/range2431919/1
component: BBa_K1638002

https://synbiohub.org/public/igem/BBa_K1638003/annotation2431919/range2431919/1
type: Range
persistent identity: range2431919
display id: range2431919
version: 1
top level: BBa_K1638003
direction: direction/reverse
start: 162
end: 848
orientation: inline

BBa_S05273
type: ComponentDefinition
persistent identity: BBa S05273
display id: BBa_S05273
version: 1
was generated by: iGEM to SBOL conversion
description: Leucine Zipper with flexible linker
created: 2015-08-17T11:00:00Z
modified: 2015-08-18T07:46:55Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 26157
owner id: 26157
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Jens Sivk??r Pettersen
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K1638016
component: BBa_K1638012
sequence annotation: BBa_K1638016
sequence annotation: BBa_K1638012
sequence: https://synbiohub.org/public/igem/BBa_S05273_sequence/1

BBa_K1638016
type: Component
persistent identity: component2436162
display id: component2436162
version: 1
top level: BBa_S05273
definition: BBa_K1638016
access: public

BBa_K1638012
type: Component
persistent identity: component2436163
display id: component2436163
version: 1
top level: BBa_S05273
definition: BBa_K1638012
access: public

BBa_K1638016
type: SequenceAnnotation
persistent identity: annotation2436162
display id: annotation2436162
version: 1
top level: BBa_S05273
location: https://synbiohub.org/public/igem/BBa_S05273/annotation2436162/range2436162/1
component: BBa_K1638016

https://synbiohub.org/public/igem/BBa_S05273/annotation2436162/range2436162/1
type: Range
persistent identity: range2436162
display id: range2436162
version: 1
top level: BBa_S05273
direction: direction/reverse
start: 1
end: 24
orientation: inline

BBa_K1638012
type: SequenceAnnotation
persistent identity: annotation2436163
display id: annotation2436163
version: 1
top level: BBa_S05273
location: https://synbiohub.org/public/igem/BBa_S05273/annotation2436163/range2436163/1
component: BBa_K1638012

https://synbiohub.org/public/igem/BBa_S05273/annotation2436163/range2436163/1
type: Range
persistent identity: range2436163
display id: range2436163
version: 1
top level: BBa_S05273
direction: direction/reverse
start: 25
end: 120
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

pLac
type: ComponentDefinition
persistent identity: BBa K1638000
display id: BBa_K1638000
version: 1
was generated by: iGEM to SBOL conversion
description: Lac operator
created: 2015-05-06T11:00:00Z
modified: 2015-05-08T01:10:55Z
mutable provenance: Coming
owned by: james
owned by: myers
mutable description: bla bla
discontinued: false
dominant: false
experience: experience/None
group u list: _2055_
m user id: 0
owner id: 26157
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Coming
star: false
creator: Jens Sivk??r Pettersen
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: CAP binding
sequence annotation: Lac promoter
sequence annotation: Lac operator
sequence: https://synbiohub.org/public/igem/BBa_K1638000_sequence/1

CAP binding
type: SequenceAnnotation
persistent identity: annotation2431869
display id: annotation2431869
version: 1
top level: pLac
location: https://synbiohub.org/public/igem/BBa_K1638000/annotation2431869/range2431869/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1638000/annotation2431869/range2431869/1
type: Range
persistent identity: range2431869
display id: range2431869
version: 1
top level: pLac
direction: direction/forward
start: 55
end: 70
orientation: inline

Lac promoter
type: SequenceAnnotation
persistent identity: annotation2431867
display id: annotation2431867
version: 1
top level: pLac
location: https://synbiohub.org/public/igem/BBa_K1638000/annotation2431867/range2431867/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1638000/annotation2431867/range2431867/1
type: Range
persistent identity: range2431867
display id: range2431867
version: 1
top level: pLac
direction: direction/forward
start: 88
end: 112
orientation: inline

Lac operator
type: SequenceAnnotation
persistent identity: annotation2431868
display id: annotation2431868
version: 1
top level: pLac
location: https://synbiohub.org/public/igem/BBa_K1638000/annotation2431868/range2431868/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K1638000/annotation2431868/range2431868/1
type: Range
persistent identity: range2431868
display id: range2431868
version: 1
top level: pLac
direction: direction/forward
start: 121
end: 142
orientation: inline

BBa_K1638001
type: ComponentDefinition
persistent identity: BBa K1638001
display id: BBa_K1638001
version: 1
was generated by: iGEM to SBOL conversion
description: RBS from pKT25 and pUT18
created: 2015-05-06T11:00:00Z
modified: 2015-05-10T01:27:55Z
mutable provenance: coming
owned by: james
owned by: myers
mutable description: coming
discontinued: false
dominant: false
experience: experience/None
group u list: _2055_
m user id: 26157
owner id: 26157
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: coming
star: false
creator: Jens Sivk??r Pettersen
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: Shine-Dalgarno
sequence: https://synbiohub.org/public/igem/BBa_K1638001_sequence/1

Shine-Dalgarno
type: SequenceAnnotation
persistent identity: annotation2431870
display id: annotation2431870
version: 1
top level: BBa_K1638001
location: https://synbiohub.org/public/igem/BBa_K1638001/annotation2431870/range2431870/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1638001/annotation2431870/range2431870/1
type: Range
persistent identity: range2431870
display id: range2431870
version: 1
top level: BBa_K1638001
direction: direction/forward
start: 9
end: 14
orientation: inline

T25 domain
type: ComponentDefinition
persistent identity: BBa K1638002
display id: BBa_K1638002
version: 1
was generated by: iGEM to SBOL conversion
description: T25 domain of CyaA from Bordetella pertussis
created: 2015-05-08T11:00:00Z
modified: 2015-05-10T01:14:50Z
mutable provenance: asd
owned by: james
owned by: myers
mutable description: This part codes for the T25 domain of the adenylate cyclase, CyaA, from Bordetella pertussis. When associated with the T18 domain of CyaA, the two domains together become active and catalyzes the conversion of ATP to cAMP
discontinued: false
dominant: false
experience: experience/None
group u list: _2055_
m user id: 26157
owner id: 26157
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: gw
star: false
creator: Jens Sivk??r Pettersen
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BamHI restriction site
sequence: https://synbiohub.org/public/igem/BBa_K1638002_sequence/1

BamHI restriction site
type: SequenceAnnotation
persistent identity: annotation2431871
display id: annotation2431871
version: 1
top level: T25 domain
location: https://synbiohub.org/public/igem/BBa_K1638002/annotation2431871/range2431871/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1638002/annotation2431871/range2431871/1
type: Range
persistent identity: range2431871
display id: range2431871
version: 1
top level: T25 domain
direction: direction/forward
start: 682
end: 687
orientation: inline

BBa_K1638016
type: ComponentDefinition
persistent identity: BBa K1638016
display id: BBa_K1638016
version: 1
was generated by: iGEM to SBOL conversion
description: 8 aa linker without BamHI restriction site and TEV recognition site
created: 2015-08-11T11:00:00Z
modified: 2015-08-12T02:01:37Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: This part is similar to the 10 aa linker with BamHI restriction site and TEV recognition site, but without the initial BamHI restriction site.
discontinued: false
dominant: false
experience: experience/None
group u list: _2055_
m user id: 26157
owner id: 26157
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Jens Sivk??r Pettersen
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K1638016_sequence/1

BBa_K1638012
type: ComponentDefinition
persistent identity: BBa K1638012
display id: BBa_K1638012
version: 1
was generated by: iGEM to SBOL conversion
description: Leucine Zipper
created: 2015-05-21T11:00:00Z
modified: 2015-08-18T07:46:42Z
mutable provenance: ..
owned by: james
owned by: myers
mutable description: ...
discontinued: false
dominant: false
experience: experience/None
group u list: _2055_
m user id: 26157
owner id: 26157
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: ..
star: false
creator: Jens Sivk??r Pettersen
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1638012_sequence/1

https://synbiohub.org/public/igem/BBa_K1638030_sequence/1
type: Sequence
persistent identity: BBa K1638030 sequence
display id: BBa_K1638030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgcagcaatcgcatcaggctggttacgcaaacgccgccgaccgggagtctggcatccccgcagccgtactcgatggcatcaaggccgtggcgaaggaaaaaaacgccacattgatgttccgcctggtcaacccccattccaccagcctgattgccgaaggggtggccaccaaaggattgggcgtgcacgccaagtcgtccgattgggggttgcaggcgggctacattcccgtcaacccgaatctttccaaactgttcggccgtgcgcccgaggtgatcgcgcgggccgacaacgacgtcaacagcagcctggcgcatggccataccgcggtcgacctgacgctgtcgaaagagcggcttgactatctgcggcaagcgggcctggtcaccggcatggccgatggcgtggtcgcgagcaaccacgcaggctacgagcagttcgagtttcgcgtgaaggaaacctcggacgggcgctatgccgtgcagtatcgccgcaagggcggcgacgatttcgaggcggtcaaggtgatcggcaatgccgccggtattccactgacggcggatatcgacatgttcgccattatgccgcatctgtccaacttccgcgactcggcgcgcagttcggtgaccagcggcgattcggtgaccgattacctggcgcgcacgcggcgggctggatccgaaaatttgtattttcaatctggtatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K861173_sequence/1
type: Sequence
persistent identity: BBa K861173 sequence
display id: BBa_K861173_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1638013_sequence/1
type: Sequence
persistent identity: BBa K1638013 sequence
display id: BBa_K1638013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgcagcaatcgcatcaggctggttacgcaaacgccgccgaccgggagtctggcatccccgcagccgtactcgatggcatcaaggccgtggcgaaggaaaaaaacgccacattgatgttccgcctggtcaacccccattccaccagcctgattgccgaaggggtggccaccaaaggattgggcgtgcacgccaagtcgtccgattgggggttgcaggcgggctacattcccgtcaacccgaatctttccaaactgttcggccgtgcgcccgaggtgatcgcgcgggccgacaacgacgtcaacagcagcctggcgcatggccataccgcggtcgacctgacgctgtcgaaagagcggcttgactatctgcggcaagcgggcctggtcaccggcatggccgatggcgtggtcgcgagcaaccacgcaggctacgagcagttcgagtttcgcgtgaaggaaacctcggacgggcgctatgccgtgcagtatcgccgcaagggcggcgacgatttcgaggcggtcaaggtgatcggcaatgccgccggtattccactgacggcggatatcgacatgttcgccattatgccgcatctgtccaacttccgcgactcggcgcgcagttcggtgaccagcggcgattcggtgaccgattacctggcgcgcacgcggcgggctggatccgaaaatttgtattttcaatctggtatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13507_sequence/1
type: Sequence
persistent identity: BBa I13507 sequence
display id: BBa_I13507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118011_sequence/1
type: Sequence
persistent identity: BBa K118011 sequence
display id: BBa_K118011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1638003_sequence/1
type: Sequence
persistent identity: BBa K1638003 sequence
display id: BBa_K1638003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgcagcaatcgcatcaggctggttacgcaaacgccgccgaccgggagtctggcatccccgcagccgtactcgatggcatcaaggccgtggcgaaggaaaaaaacgccacattgatgttccgcctggtcaacccccattccaccagcctgattgccgaaggggtggccaccaaaggattgggcgtgcacgccaagtcgtccgattgggggttgcaggcgggctacattcccgtcaacccgaatctttccaaactgttcggccgtgcgcccgaggtgatcgcgcgggccgacaacgacgtcaacagcagcctggcgcatggccataccgcggtcgacctgacgctgtcgaaagagcggcttgactatctgcggcaagcgggcctggtcaccggcatggccgatggcgtggtcgcgagcaaccacgcaggctacgagcagttcgagtttcgcgtgaaggaaacctcggacgggcgctatgccgtgcagtatcgccgcaagggcggcgacgatttcgaggcggtcaaggtgatcggcaatgccgccggtattccactgacggcggatatcgacatgttcgccattatgccgcatctgtccaacttccgcgactcggcgcgcagttcggtgaccagcggcgattcggtgaccgattacctggcgcgcacgcggcgggctggatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05273_sequence/1
type: Sequence
persistent identity: BBa S05273 sequence
display id: BBa_S05273_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaaatttgtattttcaatctggtatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1638000_sequence/1
type: Sequence
persistent identity: BBa K1638000 sequence
display id: BBa_K1638000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1638001_sequence/1
type: Sequence
persistent identity: BBa K1638001 sequence
display id: BBa_K1638001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttcacacaggaaacagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1638002_sequence/1
type: Sequence
persistent identity: BBa K1638002 sequence
display id: BBa_K1638002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgcagcaatcgcatcaggctggttacgcaaacgccgccgaccgggagtctggcatccccgcagccgtactcgatggcatcaaggccgtggcgaaggaaaaaaacgccacattgatgttccgcctggtcaacccccattccaccagcctgattgccgaaggggtggccaccaaaggattgggcgtgcacgccaagtcgtccgattgggggttgcaggcgggctacattcccgtcaacccgaatctttccaaactgttcggccgtgcgcccgaggtgatcgcgcgggccgacaacgacgtcaacagcagcctggcgcatggccataccgcggtcgacctgacgctgtcgaaagagcggcttgactatctgcggcaagcgggcctggtcaccggcatggccgatggcgtggtcgcgagcaaccacgcaggctacgagcagttcgagtttcgcgtgaaggaaacctcggacgggcgctatgccgtgcagtatcgccgcaagggcggcgacgatttcgaggcggtcaaggtgatcggcaatgccgccggtattccactgacggcggatatcgacatgttcgccattatgccgcatctgtccaacttccgcgactcggcgcgcagttcggtgaccagcggcgattcggtgaccgattacctggcgcgcacgcggcgggctggatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1638016_sequence/1
type: Sequence
persistent identity: BBa K1638016 sequence
display id: BBa_K1638016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaaatttgtattttcaatctggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1638012_sequence/1
type: Sequence
persistent identity: BBa K1638012 sequence
display id: BBa_K1638012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z