LOCUS       BBa_K1638031            1844 bp    DNA     linear       04-May-2024
DEFINITION  Leucine zipper fused to T18 domain of <i>cyaA</i> with 
            cAMP-induced RFP generator
ACCESSION   BBa_K1638031
VERSION     BBa_K1638031.1  
FEATURES             Location/Qualifiers
     misc_feature    1..42
                     /label=cAMP receptor protein binding site
     misc_feature    101..131
                     /label=RNA polymerase binding site
     promoter        1..131
                     /label=BBa_K118011
     misc_feature    144..147
                     /label=conserved
     RBS             140..151
                     /label=BBa_B0034
     CDS             158..832
                     /label=mrfp1
     misc_feature    839..863
                     /label=Help:Barcodes
     CDS             158..838
                     /label=BBa_E1010
     stem_loop       883..926
                     /label=stem_loop
     misc_feature    872..951
                     /label=BBa_B0010
     terminator      872..951
                     /label=BBa_B0010
     stem_loop       967..986
                     /label=T7 TE
     misc_feature    993..993
                     /label=stop
     polyA_site      987..1000
                     /label=polya
     misc_feature    960..1000
                     /label=BBa_B0012
     terminator      960..1000
                     /label=BBa_B0012
     misc_feature    140..1000
                     /label=BBa_I13507
     misc_feature    1..1000
                     /label=BBa_K861173
     promoter        1096..1120
                     /label=Lac promoter
     misc_feature    1129..1150
                     /label=Lac operator
     misc_feature    1063..1078
                     /label=CAP binding
     promoter        1009..1150
                     /label=BBa_K1638000
     misc_feature    1159..1164
                     /label=Shine-Dalgarno
     RBS             1151..1169
                     /label=BBa_K1638001
     misc_feature    1719..1724
                     /label=BamHI restriction site
     CDS             1170..1724
                     /label=BBa_K1638004
     misc_feature    1009..1724
                     /label=BBa_K1638005
     misc_feature    1725..1748
                     /label=BBa_K1638016
     CDS             1749..1844
                     /label=BBa_K1638012
     misc_feature    1725..1844
                     /label=BBa_S05273
     misc_feature    1009..1844
                     /label=BBa_K1638011
ORIGIN
        1 actcggttaa cggagtgatc gagttaacat tgttaagtta aatattggtt tcaactccga
       61 tttacatggt tgctgtgttg ttaaattgta caaagatgtt atagaaacaa aatgtaacat
      121 ctctatggac atactagaga aagaggagaa atactagatg gcttcctccg aagacgttat
      181 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
      241 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
      301 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
      361 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
      421 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
      481 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
      541 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
      601 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
      661 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
      721 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
      781 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
      841 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag
      901 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      961 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagct ggcacgacag
     1021 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1081 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1141 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttagccgcca
     1201 gcgaggccac gggcggcctg gatcgcgaac gcatcgactt gttgtggaaa atcgctcgcg
     1261 ccggcgcccg ttccgcagtg ggcaccgagg cgcgtcgcca gttccgctac gacggcgaca
     1321 tgaatatcgg cgtgatcacc gatttcgagc tggaagtgcg caatgcgctg aacaggcggg
     1381 cgcacgccgt cggcgcgcag gacgtggtcc agcatggcac tgagcagaac aatcctttcc
     1441 cggaggcaga tgagaagatt ttcgtcgtat cggccaccgg tgaaagccag atgctcacgc
     1501 gcgggcaact gaaggaatac attggccagc agcgcggcga gggctatgtc ttctacgaga
     1561 accgtgcata cggcgtggcg gggaaaagcc tgttcgacga tgggctggga gccgcgcccg
     1621 gcgtgccgag cggacgttcg aagttctcgc cggatgtact ggaaacggtg ccggcgtcac
     1681 ccggattgcg gcggccgtcg ctgggcgcag tggaacgcgg atccgaaaat ttgtattttc
     1741 aatctggtat gaaacagctg gaagacaaag ttgaagaact gctgtccaaa aactaccacc
     1801 tggaaaacga agttgctcgt ctgaaaaaac tggttggtga acgt
//