BBa_K1638031
1
BBa_K1638031
Leucine zipper fused to T18 domain of <i>cyaA</i> with cAMP-induced RFP generator
2015-08-17T11:00:00Z
2015-09-18T10:41:32Z
..
coming
false
false
_2055_
26157
26157
9
false
.
false
Jens Sivk??r Pettersen
component2473083
1
BBa_K861173
component2473096
1
BBa_K1638011
annotation2473083
1
BBa_K861173
range2473083
1
1
1000
annotation2473096
1
BBa_K1638011
range2473096
1
1009
1844
BBa_K1638011
1
BBa_K1638011
Leucine zipper fused to T18 domain of <i>cyaA</i> from <i>Bordetella pertussis</i>
2015-05-21T11:00:00Z
2015-09-18T10:40:52Z
..
..
false
false
_2055_
26157
26157
9
false
..
false
Jens Sivk??r Pettersen
component2473064
1
BBa_K1638005
component2473067
1
BBa_S05273
annotation2473064
1
BBa_K1638005
range2473064
1
1
716
annotation2473067
1
BBa_S05273
range2473067
1
717
836
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_I13507
1
BBa_I13507
Screening plasmid intermediate
2005-05-30T11:00:00Z
2015-08-31T04:07:34Z
Released HQ 2013
Built by Josh as an intermediate in screening plasmid construction.
false
false
_11_
0
253
6
In stock
false
true
jkm
component1524838
1
BBa_B0012
component1524822
1
BBa_E1010
component1524828
1
BBa_B0010
component1524815
1
BBa_B0034
annotation1524815
1
BBa_B0034
range1524815
1
1
12
annotation1524828
1
BBa_B0010
range1524828
1
733
812
annotation1524822
1
BBa_E1010
range1524822
1
19
699
annotation1524838
1
BBa_B0012
range1524838
1
821
861
BBa_K1638005
1
BBa_K1638005
T18 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> (IPTG inducible)
2015-05-09T11:00:00Z
2015-09-18T10:37:08Z
..
See BBa_K1638003 for description of this composite part.
false
false
_2055_
26157
26157
9
false
..
false
Jens Sivk??r Pettersen
component2431927
1
BBa_K1638001
component2431929
1
BBa_K1638004
component2431925
1
BBa_K1638000
annotation2431927
1
BBa_K1638001
range2431927
1
143
161
annotation2431925
1
BBa_K1638000
range2431925
1
1
142
annotation2431929
1
BBa_K1638004
range2431929
1
162
716
BBa_K1638012
1
BBa_K1638012
Leucine Zipper
2015-05-21T11:00:00Z
2015-08-18T07:46:42Z
..
...
false
false
_2055_
26157
26157
9
false
..
false
Jens Sivk??r Pettersen
BBa_K118011
1
BBa_K118011
PcstA (glucose-repressible promoter)
2008-08-18T11:00:00Z
2015-05-08T01:09:36Z
''Eschaerichia coli'' JM109 genomic DNA
Released HQ 2013
This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.)
false
true
_192_
0
3282
9
In stock
true
No special considerations
true
Andrew Hall
annotation1972435
1
cAMP receptor protein binding site
range1972435
1
1
42
annotation1972436
1
RNA polymerase binding site
range1972436
1
101
131
BBa_S05273
1
BBa_S05273
Leucine Zipper with flexible linker
2015-08-17T11:00:00Z
2015-08-18T07:46:55Z
false
false
_9_
26157
26157
9
false
false
Jens Sivk??r Pettersen
component2436162
1
BBa_K1638016
component2436163
1
BBa_K1638012
annotation2436162
1
BBa_K1638016
range2436162
1
1
24
annotation2436163
1
BBa_K1638012
range2436163
1
25
120
BBa_K1638000
1
pLac
Lac operator
2015-05-06T11:00:00Z
2015-05-08T01:10:55Z
Coming
bla bla
false
false
_2055_
0
26157
9
Not in stock
false
Coming
false
Jens Sivk??r Pettersen
annotation2431868
1
Lac operator
range2431868
1
121
142
annotation2431867
1
Lac promoter
range2431867
1
88
112
annotation2431869
1
CAP binding
range2431869
1
55
70
BBa_E1010
1
mRFP1
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
2004-07-27T11:00:00Z
2015-08-31T04:07:26Z
Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a>
Released HQ 2013
monomeric RFP:
Red Fluorescent Protein.
Excitation peak: 584 nm
Emission peak: 607 nm
false
false
_11_1_
0
52
7
In stock
false
TAATAA double stop codon added (DE).
Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
true
Drew Endy
annotation1014044
1
mrfp1
range1014044
1
1
675
annotation2214014
1
Help:Barcodes
range2214014
1
682
706
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K861173
1
BBa_K861173
mRFP generator controlled by PcstA (glucose-repressible promoter)
2012-09-07T11:00:00Z
2015-05-08T01:13:36Z
Escherichia coli str. K-12 substr. MG1655
Released HQ 2013
Lambda cI is controlled by promoter PcstA,which can be activated by CRP-cAMP complex.therefore protein lambda cI will be expressed under low glucose concentration.This generator contains a RFP after the promoter as a reporter.
false
false
_1121_
0
12357
9
In stock
false
None
false
Kuanwei Sheng, Xian Xia
component2193732
1
BBa_K118011
component2193744
1
BBa_I13507
annotation2193744
1
BBa_I13507
range2193744
1
140
1000
annotation2193732
1
BBa_K118011
range2193732
1
1
131
BBa_K1638004
1
T18 domain
T18 domain of CyaA from Bordetella pertussis
2015-05-09T11:00:00Z
2015-05-10T01:14:12Z
..
This part codes for the T18 domain of the adenylate cyclase, CyaA, from Bordetella pertussis. When associated with the T25 domain of CyaA, the two domains together become active and catalyzes the conversion of ATP to cAMP.
false
false
_2055_
26157
26157
9
false
..
false
Jens Sivk??r Pettersen
annotation2431921
1
BamHI restriction site
range2431921
1
550
555
BBa_K1638016
1
BBa_K1638016
8 aa linker without BamHI restriction site and TEV recognition site
2015-08-11T11:00:00Z
2015-08-12T02:01:37Z
.
This part is similar to the 10 aa linker with BamHI restriction site and TEV recognition site, but without the initial BamHI restriction site.
false
false
_2055_
26157
26157
9
false
.
false
Jens Sivk??r Pettersen
BBa_K1638001
1
BBa_K1638001
RBS from pKT25 and pUT18
2015-05-06T11:00:00Z
2015-05-10T01:27:55Z
coming
coming
false
false
_2055_
26157
26157
9
Not in stock
false
coming
false
Jens Sivk??r Pettersen
annotation2431870
1
Shine-Dalgarno
range2431870
1
9
14
BBa_S05273_sequence
1
gaaaatttgtattttcaatctggtatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgt
BBa_K1638005_sequence
1
ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttagccgccagcgaggccacgggcggcctggatcgcgaacgcatcgacttgttgtggaaaatcgctcgcgccggcgcccgttccgcagtgggcaccgaggcgcgtcgccagttccgctacgacggcgacatgaatatcggcgtgatcaccgatttcgagctggaagtgcgcaatgcgctgaacaggcgggcgcacgccgtcggcgcgcaggacgtggtccagcatggcactgagcagaacaatcctttcccggaggcagatgagaagattttcgtcgtatcggccaccggtgaaagccagatgctcacgcgcgggcaactgaaggaatacattggccagcagcgcggcgagggctatgtcttctacgagaaccgtgcatacggcgtggcggggaaaagcctgttcgacgatgggctgggagccgcgcccggcgtgccgagcggacgttcgaagttctcgccggatgtactggaaacggtgccggcgtcacccggattgcggcggccgtcgctgggcgcagtggaacgcggatcc
BBa_K1638004_sequence
1
atgaccatgattacgccaagcttagccgccagcgaggccacgggcggcctggatcgcgaacgcatcgacttgttgtggaaaatcgctcgcgccggcgcccgttccgcagtgggcaccgaggcgcgtcgccagttccgctacgacggcgacatgaatatcggcgtgatcaccgatttcgagctggaagtgcgcaatgcgctgaacaggcgggcgcacgccgtcggcgcgcaggacgtggtccagcatggcactgagcagaacaatcctttcccggaggcagatgagaagattttcgtcgtatcggccaccggtgaaagccagatgctcacgcgcgggcaactgaaggaatacattggccagcagcgcggcgagggctatgtcttctacgagaaccgtgcatacggcgtggcggggaaaagcctgttcgacgatgggctgggagccgcgcccggcgtgccgagcggacgttcgaagttctcgccggatgtactggaaacggtgccggcgtcacccggattgcggcggccgtcgctgggcgcagtggaacgcggatcc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K118011_sequence
1
actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggaca
BBa_E1010_sequence
1
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
BBa_K1638016_sequence
1
gaaaatttgtattttcaatctggt
BBa_K1638012_sequence
1
atgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgt
BBa_K1638011_sequence
1
ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttagccgccagcgaggccacgggcggcctggatcgcgaacgcatcgacttgttgtggaaaatcgctcgcgccggcgcccgttccgcagtgggcaccgaggcgcgtcgccagttccgctacgacggcgacatgaatatcggcgtgatcaccgatttcgagctggaagtgcgcaatgcgctgaacaggcgggcgcacgccgtcggcgcgcaggacgtggtccagcatggcactgagcagaacaatcctttcccggaggcagatgagaagattttcgtcgtatcggccaccggtgaaagccagatgctcacgcgcgggcaactgaaggaatacattggccagcagcgcggcgagggctatgtcttctacgagaaccgtgcatacggcgtggcggggaaaagcctgttcgacgatgggctgggagccgcgcccggcgtgccgagcggacgttcgaagttctcgccggatgtactggaaacggtgccggcgtcacccggattgcggcggccgtcgctgggcgcagtggaacgcggatccgaaaatttgtattttcaatctggtatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgt
BBa_K861173_sequence
1
actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1638001_sequence
1
tttcacacaggaaacagct
BBa_K1638000_sequence
1
ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaa
BBa_I13507_sequence
1
aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1638031_sequence
1
actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttagccgccagcgaggccacgggcggcctggatcgcgaacgcatcgacttgttgtggaaaatcgctcgcgccggcgcccgttccgcagtgggcaccgaggcgcgtcgccagttccgctacgacggcgacatgaatatcggcgtgatcaccgatttcgagctggaagtgcgcaatgcgctgaacaggcgggcgcacgccgtcggcgcgcaggacgtggtccagcatggcactgagcagaacaatcctttcccggaggcagatgagaagattttcgtcgtatcggccaccggtgaaagccagatgctcacgcgcgggcaactgaaggaatacattggccagcagcgcggcgagggctatgtcttctacgagaaccgtgcatacggcgtggcggggaaaagcctgttcgacgatgggctgggagccgcgcccggcgtgccgagcggacgttcgaagttctcgccggatgtactggaaacggtgccggcgtcacccggattgcggcggccgtcgctgggcgcagtggaacgcggatccgaaaatttgtattttcaatctggtatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z