LOCUS       BBa_K1638035            2664 bp    DNA     linear       22-Nov-2024
DEFINITION  hTrx-based scaffold fused to T18 through intein and a flexible 
            linker
ACCESSION   BBa_K1638035
VERSION     BBa_K1638035.1  
FEATURES             Location/Qualifiers
     promoter        88..112
                     /label=Lac promoter
     misc_feature    121..142
                     /label=Lac operator
     misc_feature    55..70
                     /label=CAP binding
     promoter        1..142
                     /label=BBa_K1638000
     misc_feature    151..156
                     /label=Shine-Dalgarno
     RBS             143..161
                     /label=BBa_K1638001
     misc_feature    711..716
                     /label=BamHI restriction site
     CDS             162..716
                     /label=BBa_K1638004
     misc_feature    1..716
                     /label=BBa_K1638005
     misc_feature    717..740
                     /label=BBa_K1638016
     CDS             741..2274
                     /label=BBa_K1638034
     misc_feature    717..2274
                     /label=BBa_S05289
     misc_feature    2592..2664
                     /label=3xFLAG
     misc_feature    2374..2379
                     /label=Xho res. site
     CDS             2275..2664
                     /label=BBa_K1638014
ORIGIN
        1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
       61 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      181 gcttagccgc cagcgaggcc acgggcggcc tggatcgcga acgcatcgac ttgttgtgga
      241 aaatcgctcg cgccggcgcc cgttccgcag tgggcaccga ggcgcgtcgc cagttccgct
      301 acgacggcga catgaatatc ggcgtgatca ccgatttcga gctggaagtg cgcaatgcgc
      361 tgaacaggcg ggcgcacgcc gtcggcgcgc aggacgtggt ccagcatggc actgagcaga
      421 acaatccttt cccggaggca gatgagaaga ttttcgtcgt atcggccacc ggtgaaagcc
      481 agatgctcac gcgcgggcaa ctgaaggaat acattggcca gcagcgcggc gagggctatg
      541 tcttctacga gaaccgtgca tacggcgtgg cggggaaaag cctgttcgac gatgggctgg
      601 gagccgcgcc cggcgtgccg agcggacgtt cgaagttctc gccggatgta ctggaaacgg
      661 tgccggcgtc acccggattg cggcggccgt cgctgggcgc agtggaacgc ggatccgaaa
      721 atttgtattt tcaatctggt atgtgctttg ccaagggtac caatgtttta atggcggatg
      781 ggtctattga atgtattgaa aacattgagg ttggtaataa ggtcatgggt aaagatggca
      841 gacctcgtga ggtaattaaa ttgcccagag gaagagaaac tatgtacagc gtcgtgcaga
      901 aaagtcagca cagagcccac aaaagtgact caagtcgtga agtgccagaa ttactcaagt
      961 ttacgtgtaa tgcgacccat gagttggttg ttagaacacc tcgtagtgtc cgccgtttgt
     1021 ctcgtaccat taagggtgtc gaatattttg aagttattac ttttgagatg ggccaaaaga
     1081 aagcccccga cggtagaatt gttgagcttg tcaaggaagt ttcaaagagc tacccaatat
     1141 ctgaggggcc tgagagagcc aacgaattag tagaatccta tagaaaggct tcaaataaag
     1201 cttattttga gtggactatt gaggccagag atctttctct gttgggttcc catgttcgta
     1261 aagctaccta ccagacttac gctccaattc tttatgagaa tgaccacttt ttcgactaca
     1321 tgcaaaaaag taagtttcat ctcaccattg aaggtccaaa agtacttgct tatttacttg
     1381 gtttatggat tggtgatgga ttgtctgaca gggcaacttt ttcggttgat tccagagata
     1441 cttctttgat ggaacgtgtt actgaatatg ctgaaaagtt gaatttgtgc gccgagtata
     1501 aggacagaaa agaaccacaa gttgccaaaa ctgttaattt gtactctaaa gttgtcagag
     1561 gtgctagcac aaatcctggt gtatccgctt ggcaggtcaa cacagcttat actgcgggac
     1621 aattggtcac atataacggc aagacgtata aatgtttgca gccccacacc tccttggcag
     1681 gatgggaacc atccaacgtt cctgccttgt ggcagcttca aggtggccac ggtggtattc
     1741 gcaataatct taatactgag aatccattat gggacgctat tgttggctta ggattcttga
     1801 aggacggtgt caaaaatatt ccttctttct tgtctacgga caatatcggt actcgtgaaa
     1861 catttcttgc tggtctaatt gattctgatg gctatgttac tgatgagcat ggtattaaag
     1921 caacaataaa gacaattcat acttctgtca gagatggttt ggtttccctt gctcgttctt
     1981 taggcttagt agtctcggtt aacgcagaac ctgctaaggt tgacatgaat gtcaccaaac
     2041 ataaaattag ttatgctatt tatatgtctg gtggagatgt tttgcttaac gttctttcga
     2101 agtgtgccgg ctctaaaaaa ttcaggcctg ctcccgccgc tgcttttgca cgtgagtgcc
     2161 gcggatttta tttcgagtta caagaattga aggaagacga ttattatggg attactttat
     2221 ctgatgattc tgatcatcag tttttgcttg gatctcaggt tgttgtacag aactaccatg
     2281 gtgaaacaaa ttgagtccaa gaccgccttt caagaggcac tggacgccgc gggtgacaaa
     2341 ctcgtggtcg ttgatttcag cgccacctgg ggcctcgagg gcaagatgat taaaccgttt
     2401 tttcattcgc tgtctgaaaa gtattcaaac gtgattttcc tggaggttga tgtggacgac
     2461 agccaggatg tggccagcga aagcgaagtt aaaagcatgc ctacatttca gtttttcaaa
     2521 aaaggtcaga aagttggcga atttagtggc gccaataaag aaaaattgga ggctaccatc
     2581 aacgaattgg tcatggatta caaagaccac gatggggact ataaagacca tgacattgat
     2641 tataaagatg acgacgataa gtag
//