LOCUS       BBa_K1639000             987 bp    DNA     linear       18-May-2024
DEFINITION  Toehold-GFP
ACCESSION   BBa_K1639000
VERSION     BBa_K1639000.1  
FEATURES             Location/Qualifiers
     promoter        1..19
                     /label=T7
     stem_loop       20..107
                     /label=Toehold-RNA
     RBS             61..68
                     /label=RBS
     misc_feature    87..116
                     /label=Conserved region
     misc_feature    87..107
                     /label=21 nt linker
     CDS             108..116
                     /label=GFP-9nt
     misc_feature    75..77
                     /label=ATG
     misc_feature    1..122
                     /label=BBa_K1639024
     CDS             129..842
                     /label=GFP
     misc_feature    843..881
                     /label=ASV tag
     misc_feature    129..131
                     /label=ATG
     misc_feature    882..884
                     /label=Stop Codon
     stem_loop       921..968
                     /label=T7 terminator
     misc_feature    129..987
                     /label=BBa_K1639023
ORIGIN
        1 taatacgact cactataggg tcttatctta tctatctcgt ttatccctgc atacagaaac
       61 agaggagata tgcaatgata aacgagaacc tggcggcagc gcaaaagatg cgtaaaggat
      121 cctactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      181 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      241 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccgtggc
      301 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcaca
      361 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttacgtacag gaaagaacta
      421 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
      481 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
      541 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
      601 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
      661 tagcagacca ttatcaacaa aatactccga ttggcgatgg ccctgtcctt ttaccagaca
      721 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
      781 tggtccttct tgagtttgta accgctgctg ggattacaca tggcatggat gaactataca
      841 aaaggccagc agcaaacgac gaaaactacg ctgcatcagt ttaataatta acctaggctg
      901 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      961 gttttttgct gaaaggagga actatat
//