BBa_K1639024 1 BBa_K1639024 Toehold riboregulator without reporter 2015-09-17T11:00:00Z 2015-09-21T04:46:08Z Labaroty engineered Toehold riboregulator is syntheticaly produced RNA molecule which designed complementary to trigger RNA (BBa_K1639001) This part is composed of approximately 100 nt RNA molecule. It's 30 nt is completely complementary to BBa_K1639001. Together they constitute AND logic gate part of our project. It's a new and flexible approach to AND gate switches because of sequence variablity and ease of modification. false false _2056_ 24789 24789 9 false 30 nt (87-116bp) in 3' end of molecule is very critical to function properly. We noticed it in features. false Nurgeldi Bazarov annotation2474471 1 Toehold-RNA range2474471 1 20 107 annotation2474496 1 GFP-9nt range2474496 1 108 116 annotation2474475 1 21 nt linker range2474475 1 87 107 annotation2474470 1 T7 range2474470 1 1 19 annotation2474473 1 Conserved region range2474473 1 87 116 annotation2474525 1 ATG range2474525 1 75 77 annotation2474472 1 RBS range2474472 1 61 68 BBa_K1639023 1 GFP-ASV GFP with an ASV degradation tag with a half-life of ~110 min 2015-09-17T11:00:00Z 2015-09-21T04:46:20Z This part is ASV tagged version of jellyfish Aequorea victoria. GFP is seemed to be very stable once produced. But ASV tagged GFP is susceptible to action of intracellular proteases which reduce it's half-life approximately to 110 min. It's useful for studying of real-time gene expression in individual cells. false false _2056_ 24789 24789 9 false We alreay designed it in tagged form. Anybody can express it with given sequence directly. false Nurgeldi Bazarov annotation2474667 1 T7 terminator range2474667 1 793 840 annotation2474389 1 Stop Codon range2474389 1 754 756 annotation2474388 1 ATG range2474388 1 1 3 annotation2474387 1 ASV tag range2474387 1 715 753 annotation2474386 1 GFP range2474386 1 1 714 BBa_K1639000 1 BBa_K1639000 Toehold-GFP 2015-09-11T11:00:00Z 2015-09-18T06:46:42Z Wyss Institute for Biologically Inspired Engineering. Sequence is taken from supplementary data of article: 'Toehold Switches: De-Novo-Designed Regulators of Gene Expression'. (PMID: 25417166) Toehold-GFP reporter device. This part produces reporter flourescence protein GFP, only when a specific mRNA present in the cell called Trigger RNA(BBa_K1639001). false false _2056_ 20835 20835 9 true This parts contains additional BamHI restriction site between Toehold and GFP for replacing a new gene to downstream of Toehold sequence. false Mustafa Yılmaz component2474689 1 BBa_K1639023 component2474683 1 BBa_K1639024 annotation2474689 1 BBa_K1639023 range2474689 1 129 987 annotation2474683 1 BBa_K1639024 range2474683 1 1 122 BBa_K1639000_sequence 1 taatacgactcactatagggtcttatcttatctatctcgtttatccctgcatacagaaacagaggagatatgcaatgataaacgagaacctggcggcagcgcaaaagatgcgtaaaggatcctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcacatgaaacagcatgactttttcaagagtgccatgcccgaaggttacgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccgattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaaccgctgctgggattacacatggcatggatgaactatacaaaaggccagcagcaaacgacgaaaactacgctgcatcagtttaataattaacctaggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatat BBa_K1639024_sequence 1 taatacgactcactatagggtcttatcttatctatctcgtttatccctgcatacagaaacagaggagatatgcaatgataaacgagaacctggcggcagcgcaaaagatgcgtaaaggatcc BBa_K1639023_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcacatgaaacagcatgactttttcaagagtgccatgcccgaaggttacgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccgattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaaccgctgctgggattacacatggcatggatgaactatacaaaaggccagcagcaaacgacgaaaactacgctgcatcagtttaataattaacctaggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z