BBa_K1639006 1 BBa_K1639006 PotB59-pomA 2015-09-11T11:00:00Z 2015-09-21T05:08:59Z asd PotB59, chimeric protein that is formed by the N-Terminal region of PomB and the C-terminal of E. coli MotB It was shown that a chimeric protein, PotB, renamed as PotB59, that has the N-terminal region of PomB (1???50) fused to the C-terminal periplasmic region of E. coli MotB (59???308), is functional with PomA in E. coli as well as in strains of V. alginolyticus that are defective in motX or motY (Asai et al. 2003) Instead of the hydrogen-dependant MotA MotB Stator protein partially adapted from the V. alginolyticus, sodium-dependant Stator proteins were installed. After this adaptation, the flagellar velocity increased by twice fold. false false _2056_ 24789 20835 9 false This part contains an additional BamHI restriction site at beginning of TlpB sequence and a XhoI restriction site at the end. false Mustafa Yılmaz annotation2452966 1 Ribosome Reinitiation Spacer range2452966 1 915 935 annotation2452964 1 PotB59 range2452964 1 9 911 annotation2452967 1 RBS range2452967 1 936 958 annotation2452965 1 PomA range2452965 1 965 1723 BBa_K1639006_sequence 1 gggatccgatggatgatgaggataacaagtgcgactgtcccccgccgggcctgccgttatggatggggacatttgcggatttgatgagcctgctcatgtgctttttcgtcttgctgcttagtttcagcgaaatggatgtcttgaaattcaaacaaattgcggaatattttcgcaccccgctcgcgaccgctgtgacaggtggtgaccgcatttccaactctgaaagcccgattccgggcggtggggacgactatacacaaagtcagggagaggtgaacaaacagccgaacatcgaagaactcaagaaacgtatggaacagagccgtttgcgtaagctgcgtggcgatttagatcagctcattgaatcagacccgaaacttcgtgcgctgcgcccgcatctgaagattgatctggtacaggagggtctgcgtatccagattatcgattcccaaaaccgtcccatgtttcgtacgggctcggccgatgtcgagccgtacatgcgtgatattctgcgcgccattgcacccgtgctgaatggcattccaaaccgtatcagcctgtcaggtcatacagatgacttcccgtatgcgtctggcgaaaaaggatacagcaactgggagctgtcagccgaccgcgcgaatgcgtctcgccgtgaactgatggttgggggcctggatagtggcaaagtgctgcgcgtcgttggcatggcggcgactatgcgcctgtcagatcgtggtcccgatgatgccgtgaaccgtcgtatctccctgctggtgctgaataaacaggccgagcaggccattctgcacgagaacgcagagtcgcaaaatgaaccggtgagcgcgctcgaaaagcccgaggtggccccgcaggttagcgtacccactatgccatcagcggaaccgcgttgataataacactgtataacattaagatttgtttaactttaagaaggagatataccatggatctggcaacgctgctgggcctgattggtggctttgcctttgtgattatggcgatggtgttgggagggagcattggtatgttcgtagatgttacaagcattctgattgttgtggggggcagcattttcgtggtgctcatgaaattcactatggggcagtttttcggtgcgacgaaaattgccgggaaagcatttatgttcaaagcagacgaaccggaggatctgatcgctaaaattgtggaaatggcggatgccgcccgcaaaggcgggtttttagcgcttgaagaaatggaaatcaacaataccttcatgcagaaagggatcgatctgctcgtggatggccacgacgcagatgtggtccgtgcggcactgaaaaaagatatcgccctgacggacgagcgtcatacccagggtaccggagtgttccgtgccttcggtgatgtggcaccggctatgggtatgattggcacccttgttggtttagtggcgatgctgtcgaatatggatgatccgaaagctatcggcccggccatggcagtggcccttctgaccaccctgtacggcgctatcctctcgaacatggtcttcttcccaatcgccgataaactttcactgcgccgcgaccaagaaaccttgaatcgtcgcctgattatggacggtgtactggccatccaggacggacaaaacccacgcgtaattgactcctatctgaagaattacctgaacgaaggcaaacgcgcactggaaattgacgaactcgagtaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z