BBa_K1639010 1 mLacI mammalian LacI with miR-373 binding site 2015-09-14T11:00:00Z 2015-09-15T12:20:03Z This protein is mammalian codon optimized version of LacI repressor originally derived from E.coli. It also possess nuclear localization signal (NLS) so that it can act on lac operators. In our project second step of cancer module is based on production of LacI so that it will suppress expression of DsRed. Binding site on 3' end of LacI makes it regulatable and limits it's expression only in cells with low miR-373 rate. In gastric epithelial cells level of this mirna is low but in gastric cancer cells level is very high. false false _2056_ 24789 24789 9 false We inserted two Mlu1 cut sites on each side of miR-373 binding so that other iGEM teams could easily replace it with any sequence. false Nurgeldi Bazarov annotation2457338 1 miR-373 binding site range2457338 1 1141 1163 annotation2457312 1 Kozak range2457312 1 1 6 annotation2457339 1 ATG range2457339 1 7 9 annotation2457336 1 SV40 NLS range2457336 1 1102 1122 annotation2457337 1 Stop Codon range2457337 1 1123 1134 annotation2457315 1 mammalian LacI range2457315 1 7 1125 BBa_K1639010_sequence 1 gccaccatgaaaccagttaccctgtacgatgtggccgagtacgccggggtttcttatcagaccgtcagtagagtggtgaatcaggcctcccacgtgtcagcaaagacgcgagagaaagtcgaggccgctatggccgagcttaactatattcctaatcgggtggctcagcagctcgctggtaagcagtccctgctcattggagtggctacctcctccctcgcacttcatgctccaagtcaaatagtagcagccattaaatccagagccgatcagttgggtgcttctgtggtggttagtatggtggagaggtcaggagtggaggcttgtaaagcagctgtccacaaccttctggcccagcgcgttagcggacttataattaattatcccttggacgatcaggacgctattgctgtcgaggccgcatgcacaaatgtgccagctctttttctggacgtgtccgatcagactccaattaactcaattatcttctcacacgaggatggcacccgcctgggagtggagcacctggtggctctgggacatcagcaaatcgccctgctggccggcccactgagctctgtctcagcgagactgagattggccgggtggcataagtatctcacccggaaccagatccagccgatcgctgaacgcgagggagattggtccgctatgagtggcttccagcaaaccatgcagatgttgaatgaagggatcgtgcccaccgccatgctggtagcgaacgaccagatggcacttggtgccatgcgcgcaatcacagagtctggtctccgggtgggcgccgacatctccgtggtcggttacgatgacaccgaggattcttcctgctatatcccaccgcttactacgatcaagcaggatttccggcttctgggccagacatcagtggatcgccttttgcagctgtcccagggtcaggccgtgaaggggaaccaactgttgccagtctcccttgttaaacggaaaacaactctggccccgaacacacagacagcatctccgagagcactcgctgactcactcatgcagctcgcaaggcaggtgtcacgccttgaatcaggccaatcatccctgcggccccctaagaaaaaaagaaaagtgtgatgataataaacgcgtacaccccaaaatcgaagcacttcacgcgtgcggccgcagcagcctgagacccggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z