LOCUS       BBa_K1639011            1206 bp    DNA     linear       22-Dec-2024
DEFINITION  mammalian LacI with miR-223 and miR-21 binding sites
ACCESSION   BBa_K1639011
VERSION     BBa_K1639011.1  
FEATURES             Location/Qualifiers
     RBS             1..6
                     /label=Kozak
     misc_feature    7..9
                     /label=ATG
     CDS             7..1125
                     /label=mLacI
     misc_feature    1102..1122
                     /label=SV40 NLS
     misc_feature    1123..1128
                     /label=Stop Codon
     misc_feature    1129..1150
                     /label=miR-223 binding site
     misc_feature    1180..1200
                     /label=miR-21 binding site
ORIGIN
        1 gccaccatga aaccagttac cctgtacgat gtggccgagt acgccggggt ttcttatcag
       61 accgtcagta gagtggtgaa tcaggcctcc cacgtgtcag caaagacgcg agagaaagtc
      121 gaggccgcta tggccgagct taactatatt cctaatcggg tggctcagca gctcgctggt
      181 aagcagtccc tgctcattgg agtggctacc tcctccctcg cacttcatgc tccaagtcaa
      241 atagtagcag ccattaaatc cagagccgat cagttgggtg cttctgtggt ggttagtatg
      301 gtggagaggt caggagtgga ggcttgtaaa gcagctgtcc acaaccttct ggcccagcgc
      361 gttagcggac ttataattaa ttatcccttg gacgatcagg acgctattgc tgtcgaggcc
      421 gcatgcacaa atgtgccagc tctttttctg gacgtgtccg atcagactcc aattaactca
      481 attatcttct cacacgagga tggcacccgc ctgggagtgg agcacctggt ggctctggga
      541 catcagcaaa tcgccctgct ggccggccca ctgagctctg tctcagcgag actgagattg
      601 gccgggtggc ataagtatct cacccggaac cagatccagc cgatcgctga acgcgaggga
      661 gattggtccg ctatgagtgg cttccagcaa accatgcaga tgttgaatga agggatcgtg
      721 cccaccgcca tgctggtagc gaacgaccag atggcacttg gtgccatgcg cgcaatcaca
      781 gagtctggtc tccgggtggg cgccgacatc tccgtggtcg gttacgatga caccgaggat
      841 tcttcctgct atatcccacc gcttactacg atcaagcagg atttccggct tctgggccag
      901 acatcagtgg atcgcctttt gcagctgtcc cagggtcagg ccgtgaaggg gaaccaactg
      961 ttgccagtct cccttgttaa acggaaaaca actctggccc cgaacacaca gacagcatct
     1021 ccgagagcac tcgctgactc actcatgcag ctcgcaaggc aggtgtcacg ccttgaatca
     1081 ggccaatcat ccctgcggcc ccctaagaaa aaaagaaaag tgtgataatg gggtatttga
     1141 caaactgaca acgcgtagca gcctgagacc cgcggccgct cacatcagtc tgataagcta
     1201 ggatcc
//