BBa_K1639011 1 mLacI mammalian LacI with miR-223 and miR-21 binding sites 2015-09-14T11:00:00Z 2015-09-15T12:46:07Z This protein is mammalian codon optimized version of LacI repressor originally derived from E.coli. It also possess nuclear localization signal (NLS) so that it can act on lac operators in nucleus. In our project second step of cancer module is based on production of LacI so that it will suppress expression of DsRed. Binding site on 3' end of LacI makes it regulatable and limits it's expression only in cells with low miR-223 and miR-21 levels. miR-21 has high rate of production in most cancer types. In gastric epithelial cells level of this miRNAs are low but in gastric cancer cells their level is very high. false false _2056_ 24789 24789 9 false We suggest also to view similar part we submitted-BBa_K1639010. It contains mLacI with miR binding sites but have restriction enzyme cut sites to remove miR binding site as needed. false Nurgeldi Bazarov annotation2457411 1 mLacI range2457411 1 7 1125 annotation2457422 1 SV40 NLS range2457422 1 1102 1122 annotation2457429 1 miR-21 binding site range2457429 1 1180 1200 annotation2457428 1 miR-223 binding site range2457428 1 1129 1150 annotation2457408 1 Kozak range2457408 1 1 6 annotation2457409 1 ATG range2457409 1 7 9 annotation2457427 1 Stop Codon range2457427 1 1123 1128 BBa_K1639011_sequence 1 gccaccatgaaaccagttaccctgtacgatgtggccgagtacgccggggtttcttatcagaccgtcagtagagtggtgaatcaggcctcccacgtgtcagcaaagacgcgagagaaagtcgaggccgctatggccgagcttaactatattcctaatcgggtggctcagcagctcgctggtaagcagtccctgctcattggagtggctacctcctccctcgcacttcatgctccaagtcaaatagtagcagccattaaatccagagccgatcagttgggtgcttctgtggtggttagtatggtggagaggtcaggagtggaggcttgtaaagcagctgtccacaaccttctggcccagcgcgttagcggacttataattaattatcccttggacgatcaggacgctattgctgtcgaggccgcatgcacaaatgtgccagctctttttctggacgtgtccgatcagactccaattaactcaattatcttctcacacgaggatggcacccgcctgggagtggagcacctggtggctctgggacatcagcaaatcgccctgctggccggcccactgagctctgtctcagcgagactgagattggccgggtggcataagtatctcacccggaaccagatccagccgatcgctgaacgcgagggagattggtccgctatgagtggcttccagcaaaccatgcagatgttgaatgaagggatcgtgcccaccgccatgctggtagcgaacgaccagatggcacttggtgccatgcgcgcaatcacagagtctggtctccgggtgggcgccgacatctccgtggtcggttacgatgacaccgaggattcttcctgctatatcccaccgcttactacgatcaagcaggatttccggcttctgggccagacatcagtggatcgccttttgcagctgtcccagggtcaggccgtgaaggggaaccaactgttgccagtctcccttgttaaacggaaaacaactctggccccgaacacacagacagcatctccgagagcactcgctgactcactcatgcagctcgcaaggcaggtgtcacgccttgaatcaggccaatcatccctgcggccccctaagaaaaaaagaaaagtgtgataatggggtatttgacaaactgacaacgcgtagcagcctgagacccgcggccgctcacatcagtctgataagctaggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z