LOCUS       BBa_K1639013            1944 bp    DNA     linear       20-Sep-2024
DEFINITION  pColA-KanR-dTer
ACCESSION   BBa_K1639013
VERSION     BBa_K1639013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=prefix
     misc_feature    117..752
                     /label=ColA ori
     promoter        770..861
                     /label=Amp promoter
     CDS             862..1677
                     /label=KanR
     stem_loop       1711..1839
                     /label=double terminator (B0010-B0012)
     misc_feature    1925..1944
                     /label=suffix
ORIGIN
        1 gaattcgcgg ccgcttctag agcgcggggc atgactaaca tgagaattac aacttatatc
       61 gtatggggct gacttcaggt gctacatttg aagagataaa ttgcactgaa aggtgatggt
      121 gtcgggaatc cgtaaaggat cttcttgaga tccttttacg atcgtcgtaa tctcctgctc
      181 tgtaaacgaa aaaaccgcct ggggaggcgg tttgatcgaa ggttaagtca gttggggaac
      241 tgcttaacct ggtaactggc tttagtggag cgcagatacc aaatactgtc ctttcagtgt
      301 agcctctgtt aggccaccac ttcaagactc tcgatatcta aatccactaa ttctcagtta
      361 ccaatggctg ctgccagtgg cgttttgtcg tgtctttccg ggttggactc aagatgatag
      421 ttaccggata aggcgcagca gtcgggctga acggggggtt cttgcacaca gcccagcttg
      481 gagcgaactg tctacacgga acgggacgtg gtgatttggg taaagcctcc accacaacac
      541 ggacgccgca ggacgggaac aggagagcgc aagagggagc catcaggggg aaacgcctgg
      601 tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat ttcgtgatgt
      661 tcgtcagggg ggcggagcct atggaaaaac ggcttcgctc cggccttatt gtctctctgc
      721 taagtatcct cctggcatct tctaggacgt ttctgcgcta gcatgcctat ttgtttattt
      781 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      841 taatattgaa aaaggaagag tatgagccat attcaacggg aaacgtcttg ctctaggccg
      901 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
      961 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt
     1021 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1081 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1141 gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat
     1201 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     1261 attcctgttt gtaattgtcc ttttaacagc gaccgcgtat ttcgtctcgc tcaggcgcaa
     1321 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     1381 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     1441 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     1501 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     1561 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1621 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaattg
     1681 acctaggctg ctgccactac tgagcaataa ccaggcatca aataaaacga aaggctcagt
     1741 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1801 acactggctc accttcgggt gggcctttct gcgtttatac tgaaaggagg aactataggt
     1861 ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggtt
     1921 tcagactagt agcggccgct gcag
//