LOCUS       BBa_K1639014            1488 bp    DNA     linear       22-Dec-2024
DEFINITION  TnrA-pAlsT-mRFP reporter system
ACCESSION   BBa_K1639014
VERSION     BBa_K1639014.1  
FEATURES             Location/Qualifiers
     RBS             592..614
                     /label=RBS
     CDS             9..338
                     /label=TnrA
     misc_feature    9..11
                     /label=ATG Start Codon
     stem_loop       378..424
                     /label=T7 Terminator
     promoter        458..578
                     /label=pAlsT
     CDS             621..1298
                     /label=mRFP
     misc_feature    621..623
                     /label=ATG Start Codon
     misc_feature    1335..1463
                     /label=Double Terminator (B0015)
     stem_loop       1343..1414
                     /label=rrnB T1 Terminator
     stem_loop       1430..1457
                     /label=T7 Terminator
     misc_feature    380..382
                     /label=TAA Stop Codon
     misc_feature    1337..1339
                     /label=TAA Stop Codon
ORIGIN
        1 gggatccgat gacgacggaa gatcacagct ataaagacaa gaaagtaatt agcattggca
       61 ttgtcagtga gctgaccggc ttgagcgtac gccagatccg ttactacgaa gagcgcaaac
      121 tgatctatcc tcagcgttct agccgcggga cgcgcaaata cagcttcgcg gacgtggagc
      181 gtttgatgga tatcgcgaac aaacgtgagg acggagttca gaccgccgaa attctcaaag
      241 acatgcgcaa aaaagagcag atgctgaaga acgatccgca agtacgcaag aaaatgctgg
      301 aaggtcagct taatgcgcat tttcgctaca aaaaccgtta ataattaacc taggctgctg
      361 ccaccgctga gcaataatag cataacccct tggggcctct aaacgggtct tgaggggttt
      421 tttgctgaaa ggaggaacta tatccggaag gcgccgccaa aagcaattaa tttaaatttt
      481 ttaaaaattt ctcctggatt tgatgttaag aatccttaca tcgtattgac acataatata
      541 acatcaccta taatgaaact aagttaagaa aaggaggaaa ttgaggtcga ctttgtttaa
      601 ctttaagaag gagatatacc atggcgagtt cggaggacgt tattaaagaa tttatgcgct
      661 ttaaagtccg catggaaggg agcgttaacg gccacgagtt tgaaatcgag ggcgaaggtg
      721 aagggcgccc gtacgaaggt acccagacgg ctaaattgaa agtgacgaaa ggcggcccgc
      781 tgcctttcgc atgggatatc ctgtctccgc agtttcagta cgggtcaaaa gcgtacgtga
      841 aacatccggc agatatccca gactatttga aattatcatt tccggaaggt ttcaagtggg
      901 aacgcgtgat gaatttcgaa gacggcggcg tcgttacggt tactcaggat tcatcattac
      961 aagatggtga gttcatctac aaggtgaaac ttcgtggtac caacttcccc agcgatggcc
     1021 cggtcatgca gaaaaaaacg atgggctggg aagcttctac agagcgtatg tatcccgagg
     1081 atggggccct gaaaggtgaa atcaaaatgc gcctgaaact gaaagatgga ggccactatg
     1141 atgccgaagt taagacgact tatatggcga aaaaacctgt tcaacttccg ggggcgtata
     1201 aaacagacat taaattggat attacgtctc ataatgaaga ttataccatc gtcgaacagt
     1261 atgaacgcgc tgaaggccgt cattcgaccg gcgcttaata attgacctag gctgctgcca
     1321 ctactgagca ataaccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1381 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1441 gggtgggcct ttctgcgttt atactgaaag gaggaactat actcgagt
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