LOCUS       BBa_K1639016             851 bp    DNA     linear       23-Dec-2024
DEFINITION  VP16 Transcriptional Activation Domain with miR223 and miR21 
            Binding Site
ACCESSION   BBa_K1639016
VERSION     BBa_K1639016.1  
FEATURES             Location/Qualifiers
     primer_bind     1..20
                     /label=CMV Forward Primer Binding Site
     primer_bind     832..851
                     /label=TetR Reverse Primer Binding Site
     CDS             28..261
                     /label=VP16 Transcriptional Activation Domain
     misc_feature    277..298
                     /label=miR223 Binding Site
     misc_feature    328..348
                     /label=miR21 Binding Site
     polyA_site      364..831
                     /label=SV40 polyA Signal Domain
     primer_bind     398..417
                     /label=SV40 polyA Reverse Primer Binding Site
     misc_feature    262..264
                     /label=TAG Stop Codon
ORIGIN
        1 gtaggcgtgt acggtgggag gtcgacggcc cccccgaccg atgtcagcct gggggacgag
       61 ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat
      121 ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc
      181 ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt
      241 ggaattgacg agtacggtgg gtagggggcg cgaggatggg gtatttgaca aactgacaac
      301 gcgtagcagc ctgagacccg cggccgctca catcagtctg ataagctaac tagtctgcag
      361 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
      421 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
      481 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
      541 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
      601 tgcaagcctc gtcgtcctgg ccggaccacg ctatctgtgc aaggtccccg gccccggacg
      661 cgcgctccat gagcagagcg cccgccgccg aggcgaagac tcgggcggcg ccctgcccgt
      721 cccaccaggt caacaggcgg taaccggcct cttcatcggg aatgcgcgcg accttcagca
      781 tcgccggcat gtccccctgg cggacgggaa gtatccagct cgaccaagct tgctgcttaa
      841 tgaggtcgga a
//