LOCUS BBa_K1639022 1546 bp DNA linear 25-Nov-2024 DEFINITION pCons-TetR-pTet-TevProtease Suicide Switch ACCESSION BBa_K1639022 VERSION BBa_K1639022.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 CDS 45..661 /label=tetR misc_feature 662..695 /label=SsrA misc_feature 702..726 /label=Help:Barcodes CDS 42..726 /label=BBa_C0040 misc_feature 735..753 /label=TetR 1 misc_feature 735..788 /label=BBa_R0040 promoter 754..759 /label=-35 misc_feature 760..778 /label=TetR 2 promoter 777..782 /label=-10 promoter 735..788 /label=BBa_R0040 misc_feature 805..807 /label=ATG Start Codon CDS 808..1533 /label=Tev Protease misc_feature 1534..1536 /label=Stop Codon misc_feature 1462..1464 /label=S219V CDS 797..1546 /label=BBa_K1639008 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gatgtccaga ttagataaaa 61 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa 121 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa 181 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact 241 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta 301 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag 361 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac 421 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg 481 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc 541 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct 601 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt 661 ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta 721 gatcactact agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata 781 ctgagcacta ctagagggga tccgatgggc gagtcgctgt tcaagggccc gcgtgactac 841 aacccgattt cttctacgat ttgtcatttg acaaatgaaa gtgatggtca taccaccagc 901 ctctacggca ttggattcgg cccgtttatt attacgaaca agcatttatt tcgtcgtaat 961 aacggcaccc tgctcgtgca atcactccat ggcgttttca aagtcaaaaa taccacgacc 1021 cttcaacagc accttattga tggtcgtgac atgatcatta tccgcatgcc gaaagacttt 1081 ccgccatttc cgcagaagct caagtttcgc gaacctcagc gcgaagaacg tatttgctta 1141 gtaactacca attttcagac taaaagtatg agcagcatgg tgtccgatac ttcctgtacc 1201 tttccatcat cagatggcat tttttggaaa cattggattc aaaccaaaga cggtcagtgc 1261 ggtagccctc tggtatctac acgcgatggt tttattgttg gtattcatag cgcttcgaat 1321 tttactaaca ccaacaacta ctttacgtcc gtgccaaaaa acttcatgga actgctgaca 1381 aaccaggaag cgcaacagtg ggtctcaggt tggcgtctga atgcggattc cgttttgtgg 1441 ggtggccaca aggtttttat ggtgaaaccg gaggaacctt tccagccggt caaggaagcc 1501 acccagctga tgaatgaact ggtgtatagc caataataac tcgagt //