##gff-version 3
##sequence-region BBa_K1639022 1 1546
BBa_K1639022	.	promoter	735	788	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1639022	.	promoter	754	759	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1639022	.	non_covalent_binding_site	760	778	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1639022	.	engineered_region	735	788	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1639022	.	non_covalent_binding_site	735	753	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1639022	.	promoter	777	782	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1639022	.	CDS	42	726	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K1639022	.	engineered_tag	702	726	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1639022	.	tag	662	695	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1639022	.	CDS	45	661	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1639022	.	CDS	797	1546	.	+	0	ID=BBa_K1639008;Name=BBa_K1639008
BBa_K1639022	.	start_codon	805	807	.	+	0	ID=annotation2453252;Name=ATG Start Codon;Parent=BBa_K1639008
BBa_K1639022	.	CDS	808	1533	.	+	0	ID=annotation2453253;Name=Tev Protease;Parent=BBa_K1639008
BBa_K1639022	.	stop_codon	1534	1536	.	+	0	ID=annotation2453254;Name=Stop Codon;Parent=BBa_K1639008
BBa_K1639022	.	sequence_alteration	1462	1464	.	+	0	ID=annotation2453264;Name=S219V;Parent=BBa_K1639008
BBa_K1639022	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_K1639022
ttgacggctagctcagtcctaggtacagtgctagctactagatgtccagattagataaaagtaaagtgattaacagcgc
attagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcct
acattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactc
acttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaag
tcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcc
tttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcg
tattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacg
acaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggatta
gaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagt
gctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacta
ctagaggggatccgatgggcgagtcgctgttcaagggcccgcgtgactacaacccgatttcttctacgatttgtcattt
gacaaatgaaagtgatggtcataccaccagcctctacggcattggattcggcccgtttattattacgaacaagcattta
tttcgtcgtaataacggcaccctgctcgtgcaatcactccatggcgttttcaaagtcaaaaataccacgacccttcaac
agcaccttattgatggtcgtgacatgatcattatccgcatgccgaaagactttccgccatttccgcagaagctcaagtt
tcgcgaacctcagcgcgaagaacgtatttgcttagtaactaccaattttcagactaaaagtatgagcagcatggtgtcc
gatacttcctgtacctttccatcatcagatggcattttttggaaacattggattcaaaccaaagacggtcagtgcggta
gccctctggtatctacacgcgatggttttattgttggtattcatagcgcttcgaattttactaacaccaacaactactt
tacgtccgtgccaaaaaacttcatggaactgctgacaaaccaggaagcgcaacagtgggtctcaggttggcgtctgaat
gcggattccgttttgtggggtggccacaaggtttttatggtgaaaccggaggaacctttccagccggtcaaggaagcca
cccagctgatgaatgaactggtgtatagccaataataactcgagt