BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986785
1
-35
range1986785
1
20
25
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986787
1
-10
range1986787
1
43
48
annotation1986786
1
TetR 2
range1986786
1
26
44
BBa_K1639022
1
BBa_K1639022
pCons-TetR-pTet-TevProtease Suicide Switch
2015-09-17T11:00:00Z
2015-09-18T10:18:35Z
none
This is a kill switch part of our engineering E. Coli. pTet is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. In the presence of aTc TetR repression inhibited and Tev Protease produce by Ptet promoter. Tev Protease release Pexiganan from DAMP and E. Coli kill switch works succesfully.
false
false
_2056_
20835
20835
9
false
none
false
Mustafa Yılmaz
component2472055
1
BBa_C0040
component2472056
1
BBa_R0040
component2472051
1
BBa_J23100
component2472065
1
BBa_K1639008
annotation2472055
1
BBa_C0040
range2472055
1
42
726
annotation2472065
1
BBa_K1639008
range2472065
1
797
1546
annotation2472051
1
BBa_J23100
range2472051
1
1
35
annotation2472056
1
BBa_R0040
range2472056
1
735
788
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1639008
1
BBa_K1639008
Tev Protease, single S219V mutation in the internal cleavage site
2015-09-12T11:00:00Z
2015-09-18T07:12:27Z
Tobacco etch virus (TEV).
TEV protease (also called Tobacco Etch Virus nuclear inclusion a endopeptidase) is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV).The consensus for these native cut sites is ENLYFQ\S where ???\??? denotes the cleaved peptide bond. One of the main uses of this protein is for removing affinity tags from purified proteins. The reason for the use of TEV protease as a biochemical tool is its high sequence specificity. This specificity allows for the controlled cleavage of proteins when the preference sequence is inserted into flexible loops. It also makes it relatively non-toxic in vivo as the recognized sequence scarcely occurs in proteins.[1]
false
false
_2056_
24789
20835
9
false
This part contains an additional BamHI restriction site at beginning of Tev Protease sequence and a XhoI restriction site at the end.
false
Mustafa Yılmaz
annotation2453252
1
ATG Start Codon
range2453252
1
9
11
annotation2453253
1
Tev Protease
range2453253
1
12
737
annotation2453264
1
S219V
range2453264
1
666
668
annotation2453254
1
Stop Codon
range2453254
1
738
740
BBa_C0040
1
tetR
tetracycline repressor from transposon Tn10 (+LVA)
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
Released HQ 2013
Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.</P>
false
true
_1_
0
24
7
In stock
false
References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P> References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P>BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
annotation23329
1
tetR
range23329
1
4
620
annotation2213989
1
Help:Barcodes
range2213989
1
661
685
annotation23330
1
SsrA
range23330
1
621
654
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_K1639022_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagaggggatccgatgggcgagtcgctgttcaagggcccgcgtgactacaacccgatttcttctacgatttgtcatttgacaaatgaaagtgatggtcataccaccagcctctacggcattggattcggcccgtttattattacgaacaagcatttatttcgtcgtaataacggcaccctgctcgtgcaatcactccatggcgttttcaaagtcaaaaataccacgacccttcaacagcaccttattgatggtcgtgacatgatcattatccgcatgccgaaagactttccgccatttccgcagaagctcaagtttcgcgaacctcagcgcgaagaacgtatttgcttagtaactaccaattttcagactaaaagtatgagcagcatggtgtccgatacttcctgtacctttccatcatcagatggcattttttggaaacattggattcaaaccaaagacggtcagtgcggtagccctctggtatctacacgcgatggttttattgttggtattcatagcgcttcgaattttactaacaccaacaactactttacgtccgtgccaaaaaacttcatggaactgctgacaaaccaggaagcgcaacagtgggtctcaggttggcgtctgaatgcggattccgttttgtggggtggccacaaggtttttatggtgaaaccggaggaacctttccagccggtcaaggaagccacccagctgatgaatgaactggtgtatagccaataataactcgagt
BBa_K1639008_sequence
1
gggatccgatgggcgagtcgctgttcaagggcccgcgtgactacaacccgatttcttctacgatttgtcatttgacaaatgaaagtgatggtcataccaccagcctctacggcattggattcggcccgtttattattacgaacaagcatttatttcgtcgtaataacggcaccctgctcgtgcaatcactccatggcgttttcaaagtcaaaaataccacgacccttcaacagcaccttattgatggtcgtgacatgatcattatccgcatgccgaaagactttccgccatttccgcagaagctcaagtttcgcgaacctcagcgcgaagaacgtatttgcttagtaactaccaattttcagactaaaagtatgagcagcatggtgtccgatacttcctgtacctttccatcatcagatggcattttttggaaacattggattcaaaccaaagacggtcagtgcggtagccctctggtatctacacgcgatggttttattgttggtattcatagcgcttcgaattttactaacaccaacaactactttacgtccgtgccaaaaaacttcatggaactgctgacaaaccaggaagcgcaacagtgggtctcaggttggcgtctgaatgcggattccgttttgtggggtggccacaaggtttttatggtgaaaccggaggaacctttccagccggtcaaggaagccacccagctgatgaatgaactggtgtatagccaataataactcgagt
BBa_C0040_sequence
1
atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z