BBa_K1639023
1
GFP-ASV
GFP with an ASV degradation tag with a half-life of ~110 min
2015-09-17T11:00:00Z
2015-09-21T04:46:20Z
This part is ASV tagged version of jellyfish Aequorea victoria.
GFP is seemed to be very stable once produced. But ASV tagged GFP is susceptible to action of intracellular proteases which reduce it's half-life approximately to 110 min. It's useful for studying of real-time gene expression in individual cells.
false
false
_2056_
24789
24789
9
false
We alreay designed it in tagged form. Anybody can express it with given sequence directly.
false
Nurgeldi Bazarov
annotation2474667
1
T7 terminator
range2474667
1
793
840
annotation2474389
1
Stop Codon
range2474389
1
754
756
annotation2474388
1
ATG
range2474388
1
1
3
annotation2474387
1
ASV tag
range2474387
1
715
753
annotation2474386
1
GFP
range2474386
1
1
714
BBa_K1639023_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcacatgaaacagcatgactttttcaagagtgccatgcccgaaggttacgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccgattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaaccgctgctgggattacacatggcatggatgaactatacaaaaggccagcagcaaacgacgaaaactacgctgcatcagtttaataattaacctaggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z